薜荔叶化学成分及其活性研究

采用硅胶柱层析、ODS 反相柱层析、Sephadex LH-20 凝胶柱层析、半制备高效液相 HPLC等色谱分离方法对薜荔（Ficus pumila）叶80%乙醇提取物进行分离纯化,得到7个化合物。 根据化合物的理化性质和核磁共振波谱数据比对文献,分别鉴定为：3-hydroxy-damascone （1）,月 桂酸乙酯（2）,马桑酸（3）,13-hydroxy-（9Z,11E,15E）-octadecatrienoic acid （4）,乙基-β-D-吡喃 葡萄糖苷（5）,苄醇-β-D-葡萄糖苷（6）和2-［4-（3-hydroxypropyl）-2-methoxyphenoxy］-propane- 1,3-diol （7）。其中化合物7为首次从该属植物中分离得到,化合物1为首次从该植物中分离得到。 抗肿瘤活性测试结果表明,化合物 1 对 A549 细胞显示出一定的抑制活性,IC50值为 32.21 µg/mL。 抗菌活性测试结果表明,所有化合物对测试的4株人体致病菌和2株弧菌均无明显抗菌活性。     

苯乙酰异谷氨酰胺类化合物合成及抗肿瘤活性

苯乙酰异谷酰胺作为抗癌酮（Antineoplaston A10）在体内的一种降解产物，具有一定程度的抗肿瘤活性。为此设计并合成了一些新的苯乙酰异谷氨酰胺类化合物以测试其抗肿瘤活性。合成中采用在酸性条件下，先选择性地对L－谷氨酸γ位羧基进行酯化，再进一步合成目标化合物，反应条件温和，收率好。所合成化合物结构经IR、^1HNMR及元素分析鉴定，对所合成的目标化合物的抗肿瘤活性采用MTT法对体外培养的乳腺癌细胞成活率进行了测试，初步结果显示有一定抑制肿瘤细胞存活的作用。     

棒柄花中反式-4-(1-丙烯基)-苯酚-β-D-吡喃葡萄糖苷的化学结构分离鉴定

从大戟科植物棒柄花（Cleidion brevipetiolatum Pax et Hoffm．）中分离得一化合物,经理化常数、UV、IR、^1HNMR ^13CNMR、MS等波谱测试和解析,鉴定为反式4-（1-丙烯基）-苯酚-β-D-吡喃葡萄糖苷。该化合物是首次从该植物中分离鉴定。     

鬼箭锦鸡儿细胞毒活性成分研究

 运用中压柱色谱和高效制备液相色谱方法对藏药鬼箭锦鸡儿的化学成分进行分离纯化,利用现代波谱技术鉴定所分离化合物的结构,并对所得到的化合物进行体外抗肝癌活性测试.从鬼箭锦鸡儿乙醇提取物中分离得到8个单体化合物,分别鉴定为6,7,3′-三羟基-4′-甲氧基异黄酮（1）、3,7-二羟基-4′-甲氧基黄酮（2）、3-甲氧基-4,9-二羟基紫檀素（3）、7,3′-二羟基-5′-甲氧基异黄酮（4）、6,7-二羟基-4′-甲氧基异黄酮（5）、3,9-二甲氧基-8-羟基紫檀素（6）、3,9-二甲氧基-4-羟基紫檀素（7）和3-甲氧基-4-羟基高丽槐素（8）,所有的化合物为首次从该植物中分离得到.采用MTT法对化合物1～8进行体外抗肝癌活性测试,结果显示所有的化合物对2个肝癌细胞株HepG2和Hep3B均有抑制作用,它们的IC50值范围为22.5～104.7μg/mL.     

5-去氢枞基-1,3,4-噁二唑-2-硫醇的微波合成及抗菌活性

以去氢枞酸为原料,经酰化后与水合肼反应合成去氢枞酸酰肼.在微波辐照下,去氢枞酸酰肼与二硫化碳在碱性条件下环合制得5-去氢枞基-1,3,4-噁二唑-2-硫醇.通过元素分析、IR、MS、1H NMR和13C NMR对所合成化合物进行了结构表征.体外抗菌活性测试结果表明,该化合物对革兰氏阳性菌-金黄色葡萄球菌、革兰氏阴性菌-大肠杆菌和枯草杆菌均有较强的抗菌活性,其最低抑菌浓度(MIC)分别为4,8和4/μg/mL.     

N-(4,6-二取代嘧啶-2-基)-2,4-二氯苯氧乙酰胺的合成及其生物活性测定

由2，4-二氯苯氧乙酸经酰化后得酰氯，再与2-氨基-4，6-二取代嘧啶反应制得9个未见文献报道的N-（4，6-二取代嘧啶-2-基）-2，4-二氯苯氧乙酰胺．目标化合物结构经元素分析，IR和^1H NMR确证．进行了除草活性筛选，其中2个目标化合物对单子叶和双子叶的供试杂草均显示高效的除草活性．     

叠氮法合成谷氨酰胺类化合物及其抗肿瘤活性

研究发现抗癌酮（Antineoplaston A10）降解物中谷氨酰胺与异谷氨酰胺类衍生物显示出抗肿瘤少性。为此，设计并合成了一系列苯乙酰谷氨酰胺类化合物供抗肿瘤活性筛选，采用合成酰基叠氮化合物，再与胺类于低湿下反应生成酰胺类目标化合物，该方法反应条件温和，收率好，而且手性中心不发生消旋化，所合成化合物结构经IR、^1HNMR、MS及元素分析方法鉴定。对所合成目标化合物做抗肿瘤活性评价，采用MTT法对L1210细胞进行了测试，初步结果显示有一定的抑制肿瘤细胞存活的作用。     

红榄李来源内生真菌Penicillium oxalicum HLLG-13 次级代谢产物与生物活性研究

对一株红树植物红榄李（Lumnitzera littorea（Jacq.） Voigt）内生真菌Penicillium oxalicum HLLG-13次级代谢产物进行系统研究;综合运用多种色谱分离方法结合波谱、光谱技术从中分离 鉴定了21个单体化合物,分别为2-（4-羟苯基）乙基-（2S）-羟基丙酸（1）,2-（4-羟苯基）乙酸乙酯 （2）,（4-羟 苯 基）乙 酸 甲 酯（3）,4-羟 基 苯 乙 醇（4）,2,4,5-三 甲 基 间 苯 二 酚（5）,1-（2,6- dihydroxyphenyl） butan-1-one（6）,rosmarinosin C（7）,penicyclone A（8）,penicyclone C（9）,2-（N-乙 酰氨基）苯酚（10）,N-乙酰酪胺（11）,2-［［（2S）-羟基-1-氧丙基］氨基］苯甲酰胺（12）,peniamidone A（13）,meleagrin（14）,7-羟基-2-（2ʹS-羟丙基）-5-甲基色酮（15）,3-甲基-6,8-二羟基异香豆素 （16）,（-）-6-hydroxymellein（17）,（3R）-甲基-5-氯-6,8-二羟基-二氢异香豆素（18）,（3R）-甲基-6, 8-二羟基-7-甲氧基-二氢异香豆素（19）,（-）-citreoisocoumarinol（20）和 de-O-methyldiaporthin （21）。抗菌活性测试结果表明,所有化合物对测试的5株人体致病菌和3株弧菌均无明显抗菌活 性;神经保护活性测试结果表明,化合物16具有中等的神经保护活性,在200 µmol/L浓度下,可以 将H2O2氧化损伤的HT22细胞存活率由43.36%提升至60.15%。     

双Schiff碱类化合物的合成与表征

以对羟基苯甲酸乙酯和3．5二硝基苯甲酸为原料，经多步反应合成了1种新的化合物4-（3，5-二氨基苯甲酰氧基）苯甲酸乙酯．该化合物与对羟基苯甲醛进一步反应得到1种新型的双Schiff碱类化合物，并通过IR、^1H NMR对相关化合物的结构进行了表征．     

玫瑰花中两种抗氧化成分的分离鉴定与活性测定

通过体外抗氧化活性检测寻找玫瑰花（rosarugosa）中的抗氧化活性成分．方法采用大孔树脂，硅胶柱层析分离技术对玫瑰花抗氧化活性部位进行分离纯化；并通过理化数据及色谱分析，鉴定化合物的结构．结果分离鉴定出2种抗氧化活性成分Ⅰ，Ⅱ．分别是3，5，7，3′5′-五羟基黄酮（槲皮素）和3，4，5-三羟基苯甲酸（没食子酸）．化合物Ⅰ（槲皮素）为首次从玫瑰花中分离得到．化合物Ⅰ和化合物Ⅱ的抑制小鼠红细胞溶血活性和抗小鼠组织匀浆脂质过氧化活性均高于玫瑰花粗品，     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

Wuhan University Journal of Natural Sciences TOTAL CONTENTS

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Advances in broadband wireless communications under high-mobility scenarios

Future mobile communication systems aim at providing very high data transmission rates, even in high-mobility scenarios such as high-speed wheel-track trains, maglev trains, highway vehicles, airplanes, guided missiles or spacecraft. A particularly important commercial application is the strong and increasing worldwide demand for high- speed broadband wireless communications （up to 574.8 km/ h test speeds or 380 km/h commercial speeds） in railways, providing data, voice and video services for applications such as onboard entertainment services to passengers, train control, train dispatch, train sensor status handling and sur- veillance. In such high-mobility scenarios, there are a number of communication challenges, including fast hand- over, location updating, high-speed channel modeling, estimation and equalization, anti-Doppler spreading tech- niques, fast power control, and dedicated network architec- ture. Because signal transmission in very high-speed scenarios will inevitably experience serious deterioration, it is imperative to develop key broadband mobile communi- cation techniques for such high-speed vehicles.     

Perspherical Approaches for Electrons in Atoms and Charged Excitons in Quantum Wells

Instead of following Fock’s expansion,we solve the Schrodinger equation for some quantum mechanical manybody systems such as electrons in atoms and charged excitons in quantum wells in a similar way in hyperspherical coordinates by expanding the wave functions into orthonormal complete basis sets of the hyperspherical hannonics(HHs)of hyperangles and generalized Laguerre polynomials(GLPs)of the hyperradius.This leads the equation to     

Scale-based spatial data model for GIS

Being the primary media of geographical information and the elementary objects manipulated, almost all of maps adopt the layer-based model to represent geographic information in the existent GIS. However, it is difficult to extend the map represented in layer-based model. Furthermore, in Web-Based GIS, It is slow to transmit the spatial data for map viewing. In this paper, for solving the questions above, we have proposed a new method for representing the spatial data. That is scale-based model. In this model we represent maps in three levels: scale-view, block, and spatial object, and organize the maps in a set of map layers, named Scale-View, which associates some given scales.Lastly, a prototype Web-Based GIS using the proposed spatial data representation is described briefly.     

英文诗歌中的喻类修辞

为了有助于提高英文爱好者的个人情操、文化素养和学习兴趣,以及专业人员对英语学习和研究进行多途径的探索,本文通过一些精选诗歌的引证和分析,着重论述了喻类修辞法在英文诗歌中的运用其及效果。     

Man Is Unable to Change the Miserable Life——Analysis of the Arrangement of Four Characters in The Glass Menagerie

Tennessee Williams is considered as one of the most important American playwrights since World War II.The Glass Menagerie is his first successful drama,which describes a tragic situation of family and means to say that Man is unable to change the miserable life,no matter whatever means he try,This essay focuses on the analysis of the arrangement of the four main characters:Laura.Amanda,Jim and Tom to reveal the theme.Laura is fragile.Amanda is brave.Jim is vital.Tom is sensible.And all of them develop and try the different means to struggle against life,but fail tragically.With the evidence,the paper comes to conclusion naturally that Man is unable to change the miserable life,and he dooms to fail.     

Characterization of root-nodulating bacteria on Retama raetam in arid Tunisian soils

The aim of this study is to investigate the diversity of Retama raetam root-nodule bacteria isolated from arid regions of Tunisia. Twelve isolates, chosen as representative for different 16S rRNA gene patterns, were characterized by 16S rRNA gene sequencing and phenotypic analysis. Isolates were assigned to Sinorhizobium, Rhizobium and Agrobacterium. Symbiotic properties of Sinorhizobium and Rhizobium isolates showed a large diversity in their capacity to infect their host plant and fix atmospheric nitrogen. Strain RK 22 identified as Rhizobium was the most effective isolate.     

An Affordable Accurate Evaluation of the Binding Conformations Predicted by Molecular Docking Studies in Bio-System

正Recently,docking has been widely used to predict the binding-modes of protein-inhibitors,when the crystal complexes structure was absent.Most docking algorithms are able to generate a large number of probable conformations,it,however,is difficult to effectively evaluate these docking poses and identify the most reasonable bindingmode.In the present study,on the basis of the crystallographic data of human 3-hydroxy-3-methylglutaryl coenzyme     

WIMAX与WI-FI技术分析及联合组网

介绍了WiMAX与Wi-Fi两种无线宽带接入技术,并对两者之间的关系及相互之间的影响做了对比及分析,并对WiMAX的关键技术进行了详细说明,最后对两者的联合组网方式做了简单的探讨。