短枝木麻黄地理种源遗传多样性的RAPD分析

利用RAPD分子标记对35个短枝木麻黄（Casuarina equise fifolia）种源的遗传多样性和亲缘关系进行了分析．15个随机引物共扩增了出201条DNA带．其中148条为多态性带，占73．63％．平均每个引物扩增出9．9条多态性带．种源的Shannon’s多样性指数为0．4354，Nei’s基因多样度为0．2884，表明短枝木麻黄种源的遗传多样性水平较高．35个种源彼此的遗传距离在0．0884～0．7539之间，平均0．4045．经过UPGMA聚类分析将35个种源划分为A、B、C、D、E、F6个类群。其中A群5个种源，B群17个种源，C群4个种源．D群2个种源，E群1个种源．F群6个种源．     

山西三个地区短额负蝗的RAPD分析

采用随机扩增多态性DNA(RAPD)技术对山西短额负蝗3个种群(繁峙、原平和太原)45个个体进行扩增，10条随机引物扩增共产生了125条带，多态性片段为121条，种间共有片段为5条．Nei’s基因多样性指数对RAPD数据的分析表明：种群间的遗传分化系数为0．2206，22．06％的遗传变异存在于种群间，用NJ法和UPGMA法对这三个种群的Nei’s遗传距离作聚类分析，结果显示：原平种群和繁峙种群遗传分化较小，太原种群与它们的遗传分化较大，由Nei’s遗传一致度和遗传距离可以看出，繁峙种群和原平种群的遗传距离最小为0．0852，它们与太原种群的遗传距离较远，结果表明短额负蝗不同种群的遗传分化程度与遗传距离和地理距离呈正相关。     

苔藓植物RAPD应体系的建立及遗传多样性分析

以多枝青藓为材料优化RAPD反应条件,在此基础上对11种苔藓植物进行遗传多样性分析.结果表明:苔藓植物RAPD反应(25μl体系)的最佳条件为,Taq酶,1.0U;Mg2+浓度,2.0 mmol/L;dNTP浓度,0.2 mmol/L;模板DNA,60 ng;引物浓度,10 pmol.用40条引物进行扩增筛选,有6条引物扩增条带清晰,重复性好,共扩增出77条带.通过SPSS11.5分析软件对扩增结果进行聚类分析,结果与形态学分类基本一致,说明RAPD技术可用于苔藓植物的遗传多样性研究.     

滩羊体大品系遗传标记的研究

采用RAPD(Rondom Amplified Polymorphic DNA)技术,利用混合基因池(DNA pool)法,对滩羊体大品系、普通品系进行了DNA多态性分析,从100种具有10个碱基的随机引物中,筛选出84种引物在滩羊群体基因组中共扩增出358条带,其中22种引物的扩增产物表现为多态(占22％),且扩增出32条有差异的条带,占总带数的8.94％;62种引物的扩增产物表现为单态(占62％)。滩羊体大品系的特异性条带有5条,而普通品系的特异性条带有7条,这些特异标记可以用来鉴定滩羊的两个品系;滩羊体大品系与普通品系间的遗传距离为0.136±0.087,表明两品系之间的亲缘关系很近。     

飞蝗五个自然种群的遗传分化研究

采用随机扩增多态性DNA（RAPD）技术检测飞蝗Locusta migratoria（Linnaeus）2个亚种5个种群的遗传多样性，11个随机引物扩增共产生了163条带，其中多态性片段为156条．Shannon信息指数和Nei’S指数对RAPD数据的分析表明：东亚飞蝗Locusta migratoria manilensis（Meyen）不同种群存在较高的遗传多样性；同时，东亚飞蝗种群间出现一定程度的遗传分化，遗传分化系数分别为36．09％和33．85％．用UPGMA对Nei’S遗传距离作聚类分析，结果表明：东亚飞蝗不同种群的亲缘关系较近，而它们与亚洲飞蝗Locusta migratoria migratoria（Linnaeus）关系较远．由Nei’S遗传一致度和遗传距离可以看出，东亚飞蝗不同种群间的遗传距离均小于东亚飞蝗与亚洲飞蝗之间的遗传距离．由Mantel软件检验得出地理距离和遗传距离的相关性系数r〈0．7，表明这5个种群亲缘关系的远近与地理距离无相关性．同时，还结合等位酶种群遗传结构研究结果综合分析了上述东亚飞蝗4种群的遗传多样性及其分化，表明RAPD可检测出更高水平的遗传多样性．     

果蝇突变体的RAPD分析

采用RAPD技术对遗传学模式生物——果蝇的野生型及7种突变体的遗传差异进行比较研究．结果表明，所选用的37种RAPD引物中，有25种引物扩增谱带清晰，扩增总片断284条，单个引物的扩增片段数为6～15条，平均为11．36条．不同果蝇品种间的遗传差异较小，遗传距离（D）为0．0334～0．2089．根据D值，由UPGMA聚类分析绘制了它们的分子进化树。     

葡萄的随机扩增多态性DNA研究

采用随机扩增多态性DNA(RAPD)对11个样品进行了研究．这11个品种包含1个欧美杂交种，一个中国野生种(Vitis．piasezkii Var．pagnucii)，9个欧亚种(Vitis．vinifera L)．选择了多态性好的7个10碱基寡聚糖核苷酸作为随机扩增的引物，在琼脂糖凝胶电泳上产生了76条带，其中63条具有多态性．各引物产生的条带从6到13不等，平均为10．86条／模板，其中9条具有多态性，表明葡萄基因的多态性十分丰富．通过遗传相似性分析表明11个葡萄基因的Nei氏相似性系数分布为0．1027-1．1787，平均相似系数为0．459．通过非加权算术平均数聚类法，获得了11个葡萄品种之间的遗传关系树图，欧亚种群，东亚种群和欧美杂种在遗传关系树图上被成功地聚为3组．本实验发现欧亚种和欧美杂种亲缘关系较近，而和中国野生种亲缘关系远．在欧亚种中，520A和S04的遗传距离最近，其次是井川1010和美人指，最大的遗传距离发生在桑无核和其他欧亚种之间．     

大蒜种质资源遗传多样性的分子标记研究

本研究利用RAPD和ISSR两种分子标记技术．对中国10个不同地区的大蒜品种进行了种质资源遗传多样性研究．从30个RAPD引物当中．筛选出11个具有多态性的引物．共扩增出多态性带224条．多态性条带比率为41．18％．从12个ISSR引物中筛选出5个具多态性的ISSR引物．共扩增出多态性带121条．多态性条带比率为50．21％．对RAPD和ISSR两种标记分别运用Nei指数法．计算出10个大蒜品种的平均遗传距离为0．28和0．32．根据这两种标记的结果．采用UPGMA进行聚类分析，得到与生物学分类地位基本一致的结果．结果表明，在实验稳定性上．ISSR优于RAPD．且ISSR比RAPD能检测到更多的遗传变异．RAPD和ISSR两种标记可用于大蒜种质遗传多样性的研究．     

优质红锥种源遗传多样性的RAPD分析

采用从100个随机引物中筛选出的30个有效引物，利用RAPD技术对不同地区的10个不同生态型优质红锥种源进行遗传多样性研究．结果表明：（1）30个有效引物共扩增出290条清晰谱带，平均每个引物扩增出9．67条带．多态性条带为254条，比率为87．59％；（2）10个不同生态型红锥之间的遗传相似系数介于0．5946～0．7148之间，但不同地区红锥也存在着一定的遗传差异（遗传差异在0．2852～0.4054）；（3）用引物S3扩增出的带谱可在一次实验中区别10种不同生态地区的优质红锥，同时S3也可作为10种优质红锥材料的指纹图谱，鉴定优质红锥的种源；（4）聚类分析表明，10种优质红锥种源可聚成3个独立的类群．本文作者对它们的亲缘关系进行了推测．     

中国4个两性生殖卤虫品系的ISSR指纹分析

根据微卫星DNA序列设计了10种ISSR引物，对中国4个两性生殖卤虫品系的亲缘关系进行了分析．用10种ISSR引物对卤虫基因组DNA进行扩增，共获得188条清晰稳定的条带，75个基因座位，其中有99条多态性片段，53个多态性座位，片段长度在2000-150bp之间．聚类分析结果表明：3个内蒙品系卤虫亲缘关系较近，当遗传相似系数为0．5333时，明显把来自内蒙古和西藏的卤虫分为2个类群．     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.     

Tracking data management using topology of moving object database system for moving object constrained trajectory in LBS application

Advances in wireless technologies and positioning technologies and spread of wireless devices, an interest in LBS (Location Based Service) is arising. To provide location based service, tracking data should have been stored in moving object database management system (called MODBMS) with proper policies and managed efficiently. So the methods which acquire the location information at regular time intervals then, store and manage have been studied. In this paper, we suggest tracking data management techniques using topology that is corresponding to the moving path of moving object. In our techniques, we update the MODBMS when moving object arrived at a street intersection or a curved road which is represented as the node in topology and predict the location at past and future with attribute of topology and linear function. In this technique, location data that are corresponding to the node in topology are stored, thus reduce the number of update and amount of data. Also in case predicting the location,because topology are used as well as existing location information, accuracy for prediction is increased than applying linear function or spline function.