水稻矮缩病毒基因组分析与部分基因片段的表达及功能研究

根据本实验室对水稻矮缩病毒中国福建分离株基因组全序列的测定。分析了其编码蛋白的功能,并将ＲＤＶＳ６,Ｓ９,Ｓ１０,Ｓ１１编码区ｃＤＮＡ分别克隆到表达载体ｐＴｒｃＨｉｓＡ,ｐＢＶ２２１,ｐＴｒｃＨｉｓＢ和ｐＢＶ２２１中,构建成表在挝 ｐＴＨ－Ｓ６,ｐＢＶＰ９,ｐＴＨ－２１０,ｐＢ－ＶＰ１１。     

胸苷5′位修饰物的合成、表征及抗HIV活性

合成了两个胸苷５’位修饰物,即５’－叠氮－胸苷和５’－氨基－胸苷,以及３’－－脱氧－２’－３’－二脱氢胸苷,对照物通过ＩＲ,ＵＶ／ｖｉｓ,＾１ＨＮＭＲ和ＭＳ等进行了表征。     

三种脱氧核苷5＇-O-对甲苯磺酸酯的合成

首次合成了５’－０－对四一磺酰基－２’，３’－脱氢－２’，３’－二脱氧腺苷、５’０Ｏ）对甲苯磺酰基－２’，３’－二脱尿苷、５’－Ｏ－对甲苯磺酰基－２’，３’０脱氧尿苷等三个对甲苯磺酰化的脱氧核苷修饰物，并通过＾１ＨＮＭＲ、ＵＶ．Ｖｉｓ、ＭＳ等方法进行了表征。     

小鼠免疫脾细胞与SP2／0细胞融合的杂交瘤细胞的超微结构研究

对ＳＰ２／０骨髓瘤细胞、免疫脾细胞和由二者融合而分泌抗水貂肠炎病毒（ＭｉｎｋＥｎｔｅｒｔｉｔｉｓＶｉｒｕｓ，ＭＥＶ）单克隆抗体的杂交瘤细胞超微结构进行了研究．经ＭＥＶ免疫的动物的脾细胞糙面内质网发达，细胞质与细胞核的比例增大．贴壁生长的ＳＰ２／０细胞的表面中央为微绒毛集中区，其余部分则遍布皱褶，细胞质中内质网不发达．由ＳＰ２／０细胞和免疫牌细胞融合形成的杂交瘤细胞，表面均匀分布有皱褶，无微绒毛，细胞在贴壁基部周围伸出裙状伪足，扩大了贴壁面积，使细胞贴壁较牢固．本实验还对ＳＰ２／０细胞和杂交瘤细胞的染色体做了计数，二者的平均数分别为６７和９７，在传代培养中染色体有丢失现象．     

钢结构构件设计的CAD技术

介绍了在钢结构构件设计中的一些ＣＡＤ技术,主要是通过利用Ｖｉｓｕａｌ Ｂａｓｉｃ来编制钢结构,并与建筑结构设计软件接口,使钢结构的构件计算与截面选择均由为自动完成,从而大大减少了在钢结构设计中的大量繁琐计算,进而提高了钢结构的设计效率,为了便于计算机直接选择钢结构构件截面,该过程还将利用ＡＣＣＥＳＳ９７来编制钢结构材料库。     

用VisualC＋＋创建DLL实现PC机与单片机间双向数据通讯的 …

用ＶｉｓｕａｌＣ＋＋创建动态链接库ＤＬＬ,拓展了ＶｉｓｕａｌＢａｓｉｃｆｏｒＷｉｎｄｏｗｓ的功能,使ＶＢ具有访问低层端口的能力,利用标准的ＲＳ２３２Ｃ接口芯片ＩＮＳ８２５０,实现了ＰＣ机与单片机的双向数据传输。     

Sn－SnO_x薄膜的湿敏特性研究

研究了利用ＶＥＴＯ技术获得的一种新的Ｓｎ－ＳｎＯｘ电阻式薄膜湿度传感器；湿敏薄膜包含β－Ｓｎ、ＳｎＯ和ＳｎＯ２态，ＳＥＭ观察表明，孔尺寸在０．１～０．５μｍ之间的表面结构对应着高性能的湿度传感器，环境湿度从１１％～９７％ＲＨ的变化过程中，传感器阻抗变化约三个量级，传感器具有小子３０ｓ的恢复时间。     

单纯疱疹病毒胸苷激酶基因的脑肿瘤基因治疗研究

单纯疱疹病毒胸苷激酶基因（ｈｅｒｐｅｓＳｉｍｐｌｅｘＶｉｒｕｓ－ＴｈｙｍｉｄｉｎｅＫｉｎａｓｅ，ＨＳＶ－ｔｋ），配合核苷酸类似物（ＮＡＳ）ＧＣＶ或ＡＣＶ的基因治疗是目前肿瘤基因治疗领域中的一种较有然望的治疗方法。     

“97”后香港特区发展高科技产业的意义和策略

“９７”后香港特区发展高科技产业的意义和策略ＳｉｇｎｉｆｉｃａｎｃｅａｎｄＳｔｒａｔｅｇｙｏｆＤｅｖｅｌｏｐｍｅｎｔｏｆＨｉ－ＴｅｃｈＩｎｄｕｓｔｒｉｅｓｉｎＨｏｎｇｋｏｎｇＳｐｅｃｉａｌＡｄｍｉｎｉｓｔｒａｔｉｖｅＡｒｅａａｆｔｅｒ１９９７￥／／廖...     

具全期启动子盒的杆状病毒高效表达载体的组建与应用

通过ＰＣＲ扩增，得到苜蓿丫纹夜蛾核型多角体病毒（ＡｕｔｏｇｒａｐｈａｃａｌｉｆｏｒｎｉｃａＮｕｃｌｅａｒＰｏｌｙｈｅｄｒｏｓｉｓＶｉｒｕｓ，ＡｃＮＰＶ）具早晚期启动子元件的ｐ３５基因启动子，将其插入到杆状病毒转移载体质粒ｐＳＸＩＶＶＩ＋Ｘ３多克隆位点上游，使之与ｐＳＸＩＶＶＩ＋Ｘ３质粒中的人工合成后期启动子（ＰＳｙｎ）、多角体ＸＩＶ启动子（ＰＸＩＶ）串联构成早期、晚期、极晚期能持续启动外源基因表达的转移载体质粒ｐＳＸ３５．将ｐＳＸ３５用于组建含ＨＢｓＡｇ基因并形成多角体的重组ＴｎＮＰＶ，ＨＢ－ｓＡｇ基因的表达量显著提高，表达时间亦明显提前，从而实现了外源基因在杆状病毒表达系统的全期、高效表达．ｍＲＮＡ引物延伸试验结果显示，Ｐｐ３５在重组病毒中可产生２套转录本，分别于病毒感染的早期和晚期起始ＨＢｓＡｇ基因的表达．     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

Wuhan University Journal of Natural Sciences TOTAL CONTENTS

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Advances in broadband wireless communications under high-mobility scenarios

Future mobile communication systems aim at providing very high data transmission rates, even in high-mobility scenarios such as high-speed wheel-track trains, maglev trains, highway vehicles, airplanes, guided missiles or spacecraft. A particularly important commercial application is the strong and increasing worldwide demand for high- speed broadband wireless communications （up to 574.8 km/ h test speeds or 380 km/h commercial speeds） in railways, providing data, voice and video services for applications such as onboard entertainment services to passengers, train control, train dispatch, train sensor status handling and sur- veillance. In such high-mobility scenarios, there are a number of communication challenges, including fast hand- over, location updating, high-speed channel modeling, estimation and equalization, anti-Doppler spreading tech- niques, fast power control, and dedicated network architec- ture. Because signal transmission in very high-speed scenarios will inevitably experience serious deterioration, it is imperative to develop key broadband mobile communi- cation techniques for such high-speed vehicles.     

Perspherical Approaches for Electrons in Atoms and Charged Excitons in Quantum Wells

Instead of following Fock’s expansion,we solve the Schrodinger equation for some quantum mechanical manybody systems such as electrons in atoms and charged excitons in quantum wells in a similar way in hyperspherical coordinates by expanding the wave functions into orthonormal complete basis sets of the hyperspherical hannonics(HHs)of hyperangles and generalized Laguerre polynomials(GLPs)of the hyperradius.This leads the equation to     

Scale-based spatial data model for GIS

Being the primary media of geographical information and the elementary objects manipulated, almost all of maps adopt the layer-based model to represent geographic information in the existent GIS. However, it is difficult to extend the map represented in layer-based model. Furthermore, in Web-Based GIS, It is slow to transmit the spatial data for map viewing. In this paper, for solving the questions above, we have proposed a new method for representing the spatial data. That is scale-based model. In this model we represent maps in three levels: scale-view, block, and spatial object, and organize the maps in a set of map layers, named Scale-View, which associates some given scales.Lastly, a prototype Web-Based GIS using the proposed spatial data representation is described briefly.     

英文诗歌中的喻类修辞

为了有助于提高英文爱好者的个人情操、文化素养和学习兴趣,以及专业人员对英语学习和研究进行多途径的探索,本文通过一些精选诗歌的引证和分析,着重论述了喻类修辞法在英文诗歌中的运用其及效果。     

Man Is Unable to Change the Miserable Life——Analysis of the Arrangement of Four Characters in The Glass Menagerie

Tennessee Williams is considered as one of the most important American playwrights since World War II.The Glass Menagerie is his first successful drama,which describes a tragic situation of family and means to say that Man is unable to change the miserable life,no matter whatever means he try,This essay focuses on the analysis of the arrangement of the four main characters:Laura.Amanda,Jim and Tom to reveal the theme.Laura is fragile.Amanda is brave.Jim is vital.Tom is sensible.And all of them develop and try the different means to struggle against life,but fail tragically.With the evidence,the paper comes to conclusion naturally that Man is unable to change the miserable life,and he dooms to fail.     

Characterization of root-nodulating bacteria on Retama raetam in arid Tunisian soils

The aim of this study is to investigate the diversity of Retama raetam root-nodule bacteria isolated from arid regions of Tunisia. Twelve isolates, chosen as representative for different 16S rRNA gene patterns, were characterized by 16S rRNA gene sequencing and phenotypic analysis. Isolates were assigned to Sinorhizobium, Rhizobium and Agrobacterium. Symbiotic properties of Sinorhizobium and Rhizobium isolates showed a large diversity in their capacity to infect their host plant and fix atmospheric nitrogen. Strain RK 22 identified as Rhizobium was the most effective isolate.     

An Affordable Accurate Evaluation of the Binding Conformations Predicted by Molecular Docking Studies in Bio-System

正Recently,docking has been widely used to predict the binding-modes of protein-inhibitors,when the crystal complexes structure was absent.Most docking algorithms are able to generate a large number of probable conformations,it,however,is difficult to effectively evaluate these docking poses and identify the most reasonable bindingmode.In the present study,on the basis of the crystallographic data of human 3-hydroxy-3-methylglutaryl coenzyme     

WIMAX与WI-FI技术分析及联合组网

介绍了WiMAX与Wi-Fi两种无线宽带接入技术,并对两者之间的关系及相互之间的影响做了对比及分析,并对WiMAX的关键技术进行了详细说明,最后对两者的联合组网方式做了简单的探讨。