犬细小病毒单克隆抗体在治疗上的应用研究

本试验采用犬细小病毒单克隆抗体（ＣａｎｉｎｅＰａｒｖｏｖｉｒｕｓＭｏｎｏｃｌｏｎａｌＡｎｔｉｂｏｄｙ，ＣＰＶＭｃＡｂ）对２８７例患细小病毒性肠炎的病犬，经股内侧肌肉注射进行治疗，取得令人满意的结果。１．在收治的２８７例病犬中，治愈２７９例，治愈率高达９７．２１％，尤其是小于３月龄的幼犬，治愈率高达９７．９２％，显著高于其他治疗方法的治疗效果。２．在收冶的２８７例病犬中，６月龄以下幼犬占９３．７３％。冬季发病犬占９２．６８％，说明本病主要发生于６月龄以下幼犬，冬季发病较多，其次为初春或深秋，夏季发病较少。３．在临床治疗中发现，发病一天的病犬仅注射ＣＰＶＭｃＡｂ，而不采取其他对症措施，病犬即可在２天内完全康复。发病３天以内的病犬２４１只，在注射ＣＰＶＭ＿－ｃＡｂ同时，结合对症措施，治愈２４Ｏ只，仅死亡１只。随病程延长，治愈率下降。另外，８８．５３％的治愈犬在注射ＣＰＶＭｃＡｂ４天内完全康复，说明采用ＣＰＶＭｃＡｂ治疗还可缩短病程。４，注射ＣＰＶＭｃＡｂ后７ｈ以内死亡的病犬，主要是由于发病后期，全身性衰竭、肠道广泛性出血、坏死和严重脱水，注射的ＣＰＶＭｃＡｂ未能起作用。３天后死亡的病犬，可能是由于ＣＰＶＭｃＡｂ虽     

应用聚合酶链式反应检测犬细小病毒

将具有犬细小病毒病典型症状的病犬肠溶物经ＳＤＳ－酚裂解法提取总ＤＮＡ,并以此ＤＮＡ为模板,通过人工合成的两条２０ｍｅｒ引物进行ＰＣＲ扩增,该对引物扩增的片段为犬细小病毒结构蛋白ＶＰ２基因中间１／３区段,ＰＣＲ产物经琼脂糖凝胶电泳,试验结果表明：用ＰＣＲ扩增检测犬细小病毒具有良好的特异性,应用该方法检测的２７份病犬肠溶物样品块获得了预计长度的ＤＮＡ片段（５３１ｂｐ）,而健康犬的肠溶物样品ＰＣＲ结果为     

用PCR扩增和克隆鸡贫血病毒衣壳蛋白VP2基因

接种鸡贫血病毒（ＣＡＶ）Ｃｕｘ－１毒株于ＭＤＣＣ－ＲＰ１细胞中,并用经狄高辛标记的ＣＡＶ衣壳蛋白ＶＰ１基因克隆ＤＮＡ作为探针在斑点杂交中检测和比较了各代细胞中ＣＡＶ ＤＮＡ水平。通过聚合酶链式反应（ＰＣＲ）,从ＣＡＶ ＤＮＡ水平较高的ＭＤＣＣ－ＲＰ１细胞基因组ＤＮＡ中扩增出ＣＡＶ衣壳蛋白ＶＰ２基因片段约６５０ｂｐ的编码序列,将该ＰＣＲ扩增的产物于ＥｃｏＲＩ和ＫｐｎＩ位点克隆到ｐＵＣ１９质粒载体中,     

以充气背心为主体的新型双泵复苏术血流动力学研究(2)

以充气背心为主体的新型双泵复苏术（ＶＤＶＥＳＴ－ＣＰＲ）能改善冠脉有效灌注压（ＣＰＰ），观测其复苏效果随时间变化．以ＩＢＭＰＣ３８６为控制主机的多功能复苏装置，在８条犬心室颤动３０ｓ后随机地实施充气背心复苏术（ＶＥＳＴ－ＣＰＲ）及ＶＤＶＥＳＴ－ＣＰＲ３０ｍｉｎ，每隔５ｍｉｎ观测一次，自身对照设计．结果：整个实验中，ＶＤＶＥＳＴ－ＣＰＲ较ＶＥＳＴ－ＣＰＲ显著升高主动脉和右房的舒张压（Ｐ＜０．０１）．主动脉舒张压升高程度较右房舒张压大，ＣＰＰ显著性升高（Ｐ＜０．０１）．虽然随复苏时间延长，复苏效果有所下降，但是ＶＤＶＥＳＴ－ＣＰＲ始终优于ＶＥＳＴ－ＣＰＲ．ＶＤＶＥＳＴ－ＣＰＲ３０ｍｉｎ的复苏中，血流动力学效果优于ＶＥＳＴ－ＣＰＲ．     

应用PCR－RFLP法作脊灰炎病毒型内分析的研究

用ＰＣＲ－ＲＦＬＰ法对近年来从麻痹病人分离到的２６株脊灰炎病毒（ＰＶ）作了型内分析研究。１６株ＰＶ１的ＲＦＬＰ图式呈现多样性，毒株的图式可与Ｓａｂｉｎ１型疫苗株完全相同或不同，某些毒株亦可无电泳条带出现。７株ＰＶ２与３株ＰＶ３的ＲＦＬＰ图式则分别与疫苗株Ｓａｂｉｎ２型和３型完全相同。全面分析这些ＲＦＬＰ后，我们认为１６株ＰＶ１为野毒株，而７株ＰＶ２及３株ＰＶ３则可能是疫苗相似株。鉴于近年来广西的麻痹病例仍主要由ＰＶ１所致。故此，加强突击普服ＯＰＶ运动及作好分离毒株的型内分析已成为消灭脊灰炎的关键。     

中蜂囊状幼虫病病毒部分结构蛋白基因的克隆及序列分析

根据小ＲＮＡ 病毒科（ Ｐｉｃｏｒｎａｖｉｒｉｄａｅ） 中病毒ＲＮＡ 所具有的结构特征, 采用ｍＲＮＡｃａｐｔｕｒｅ ｋｉｔ 提取纯化中蜂囊状幼虫病病毒（Ｃｈｉｎｅｓｅｓｃａｂｒｏｏｄ ｖｉｒｕｓ ＣＳＢＶ） 的ＲＮＡ, 并以之为ｃＤＮＡ合成的模板． 依据小ＲＮＡ病毒科中的脊髓灰质炎病毒结构蛋白基因序列设计了一对引物ＶＰ５和ＶＰ３ , 通过ＰＣＲ 扩增获得预期大小约为１ １００ ｂｐ的ＤＮＡ 片段, 将此片段克隆到ｐＧＥＭＴｅａｓｙ载体上并直接测序． 序列分析表明, 该片段为中蜂囊状幼虫病病毒部分结构蛋白基因, 与意蜂幼虫囊状病病毒结构蛋白基因序列的同源性为８６－８ ％ , 与之对应氨基酸序列的同源性高达９３－４ ％ ． 该病毒株为一种新型的蜜蜂囊状幼虫病病毒株     

不同月龄Beagle犬的心电图研究

对不同月龄的６６只健康Ｂｅａｇｌｅ犬采用３％戊巴比妥钠麻醉，进行了９个导联的心电图描记和分析。结果表明：健康Ｂｅａｇｌｅ犬为窦性心律：３０月龄以下育成犬心律平均为１７３．８次／分，种用犬（５０月龄以上）心率平均为１３１．７次／分；二者经统计学处理，有极显著差异。Ｐ波间期平均为０．０５１±０．０１３Ｓｅｃ。Ｐ波振幅平均０．０６１ｍｖ，Ｐ波直立在导联中占９２．６％，在ａＶＲ导联中Ｐ波倒置率９８．７５％；ＱＲＳ波间期均值为：０．０２７±０．００５Ｓｅｃ。在Ⅱ导联中范围为０．０２－０．０４Ｓ－ｅｃ。在各导联中，Ⅰ导联以ｒ波为主（５１．８５％），Ⅱ导联以Ｒｓ波多见（５０％），Ｑｒ波和ｑｒ波在ａＶＲ导联中出现较多（３８．８９％，３１．４８％）。Ｔ波在Ⅰ、Ⅱ、Ⅲ、ａｖＦ、Ⅴ１、Ⅴ２、Ⅴ３导联中直立为主，在ａｖＬ导联中倒置多见（８９．２％，７３．１％）。Ｔ波方向与ＱＲＳ波一致。Ｔ波振幅平沟０．１３７ｍｖ。Ｐ－Ｒ间期范围在此０。０７５－０．１４０Ｓｅｃ。在９个导联中，无明显Ｓ－Ｐ段偏移。Ｐ－Ｐ间隔＜０．０１Ｓｅｃ。     

比格犬柯萨奇病毒感染的报道

柯萨奇Ｂ组病毒（ＣｏｘｓａｃｋｉｅＢＶｉｒｕｓｅｓＣＢＶ）是一种人兽共患病毒。ＣＢＶ引起神经系统病变时人与比格犬所表现的症状基本相似。ＣＢＶ可引起全身性疾病,损伤多系统。应注意早期的诊断和治疗。ＣＢＶ并非终生免疫,应注意再次感染     

大肠杆菌β－半乳糖苷酶基因在小菜蛾细胞中的表达

用携带大肠杆菌β－半乳糖苷酶（β－ｇａｌ）基因的杆状病毒转移载体．ｐＡｃ３６０－β－ｇａｌ与苜蓿银纹夜蛾核型多角体病毒（ＡｃＮＰＶ）ＤＮＡ经钙磷沉淀法共转染小菜蛾细胞系（ＢＣＩＲＬ－ＰＸ２－ＨＮＵ２，Ｐｘ），利用Ｘ－ｇａｌ空斑检测分析，β－ｇａｌ基因成功地插入ＡｃＮＰＶ基因组中，得到重组病毒Ａｃ－β－ｇａｌ，重组病毒在Ｐｘ细胞中表达出受ＡｃＮＰＶ多角体蛋白基因启动子控制的具有生物活性的外源基因表达产物──β－ｇａｌ．     

腺病毒同源重组子的鉴定

携带入ＩＦＮ－γｃＤＮＡ序列的Ｅ１区缺失的腺病毒载体ｐＡｄｌ２／ＲＳＶ－ＩＦＮ－ｂｐＡ与ｐＪＭ１７质粒，通过磷酸钙沉淀法共转染２９３细胞，经同源重组，共获得６个病毒噬斑。病毒噬斑感染２９３细胞，至出现完全细胞病变效应（ＣＰＥ）后，抽提细胞内的病毒ＤＮＡ，经ＸｈｏⅠ酶切后走凝胶电泳，出现重组腺病毒圾的３．５ｋｂ大小的ＤＮＡ条带，＾３２Ｐ标记的探针ｐＡｄｌ２／ＲＳＶ－ＩＦＮ－ｂｐＡ与ＸｈｏⅠ酶切过的病     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

Wuhan University Journal of Natural Sciences TOTAL CONTENTS

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Advances in broadband wireless communications under high-mobility scenarios

Future mobile communication systems aim at providing very high data transmission rates, even in high-mobility scenarios such as high-speed wheel-track trains, maglev trains, highway vehicles, airplanes, guided missiles or spacecraft. A particularly important commercial application is the strong and increasing worldwide demand for high- speed broadband wireless communications （up to 574.8 km/ h test speeds or 380 km/h commercial speeds） in railways, providing data, voice and video services for applications such as onboard entertainment services to passengers, train control, train dispatch, train sensor status handling and sur- veillance. In such high-mobility scenarios, there are a number of communication challenges, including fast hand- over, location updating, high-speed channel modeling, estimation and equalization, anti-Doppler spreading tech- niques, fast power control, and dedicated network architec- ture. Because signal transmission in very high-speed scenarios will inevitably experience serious deterioration, it is imperative to develop key broadband mobile communi- cation techniques for such high-speed vehicles.     

Perspherical Approaches for Electrons in Atoms and Charged Excitons in Quantum Wells

Instead of following Fock’s expansion,we solve the Schrodinger equation for some quantum mechanical manybody systems such as electrons in atoms and charged excitons in quantum wells in a similar way in hyperspherical coordinates by expanding the wave functions into orthonormal complete basis sets of the hyperspherical hannonics(HHs)of hyperangles and generalized Laguerre polynomials(GLPs)of the hyperradius.This leads the equation to     

Scale-based spatial data model for GIS

Being the primary media of geographical information and the elementary objects manipulated, almost all of maps adopt the layer-based model to represent geographic information in the existent GIS. However, it is difficult to extend the map represented in layer-based model. Furthermore, in Web-Based GIS, It is slow to transmit the spatial data for map viewing. In this paper, for solving the questions above, we have proposed a new method for representing the spatial data. That is scale-based model. In this model we represent maps in three levels: scale-view, block, and spatial object, and organize the maps in a set of map layers, named Scale-View, which associates some given scales.Lastly, a prototype Web-Based GIS using the proposed spatial data representation is described briefly.     

英文诗歌中的喻类修辞

为了有助于提高英文爱好者的个人情操、文化素养和学习兴趣,以及专业人员对英语学习和研究进行多途径的探索,本文通过一些精选诗歌的引证和分析,着重论述了喻类修辞法在英文诗歌中的运用其及效果。     

Man Is Unable to Change the Miserable Life——Analysis of the Arrangement of Four Characters in The Glass Menagerie

Tennessee Williams is considered as one of the most important American playwrights since World War II.The Glass Menagerie is his first successful drama,which describes a tragic situation of family and means to say that Man is unable to change the miserable life,no matter whatever means he try,This essay focuses on the analysis of the arrangement of the four main characters:Laura.Amanda,Jim and Tom to reveal the theme.Laura is fragile.Amanda is brave.Jim is vital.Tom is sensible.And all of them develop and try the different means to struggle against life,but fail tragically.With the evidence,the paper comes to conclusion naturally that Man is unable to change the miserable life,and he dooms to fail.     

Characterization of root-nodulating bacteria on Retama raetam in arid Tunisian soils

The aim of this study is to investigate the diversity of Retama raetam root-nodule bacteria isolated from arid regions of Tunisia. Twelve isolates, chosen as representative for different 16S rRNA gene patterns, were characterized by 16S rRNA gene sequencing and phenotypic analysis. Isolates were assigned to Sinorhizobium, Rhizobium and Agrobacterium. Symbiotic properties of Sinorhizobium and Rhizobium isolates showed a large diversity in their capacity to infect their host plant and fix atmospheric nitrogen. Strain RK 22 identified as Rhizobium was the most effective isolate.     

Features of Women''s language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

An Affordable Accurate Evaluation of the Binding Conformations Predicted by Molecular Docking Studies in Bio-System

正Recently,docking has been widely used to predict the binding-modes of protein-inhibitors,when the crystal complexes structure was absent.Most docking algorithms are able to generate a large number of probable conformations,it,however,is difficult to effectively evaluate these docking poses and identify the most reasonable bindingmode.In the present study,on the basis of the crystallographic data of human 3-hydroxy-3-methylglutaryl coenzyme