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1.
黄原胶适宜培养条件的研究   总被引:1,自引:0,他引:1  
王浩  杨明慧 《山东科学》1997,10(1):49-52,57
分别研究了野油菜黄单胞菌SD-2液体种子培养和发酵产胶的适宜条件。液体种子培养的适宜条件为:起始pH7.0,CaCo3i浓度0,培养温度29℃,500mL三角瓶装量100mL,发酵产胶的适宜条件为:起始pH7.,CaCo3浓度0.2%,培养温度31℃,500mL。采用适宜条件发酵黄原胶,最终发酵液粘度为8720mPa.s,黄原胶浓度为2.78%,分别比对照提高了25.3%和15.4%。  相似文献   

2.
以甘蔗糖蜜为碳源生产黄原胶,摇瓶发酵72h,其产胶量可达3.26%,碳源转化率为56.2%,成品粘度1760mPa.s,剪切值6.53。实验结果表明,甘蔗糖蜜完全可以代替蔗糖、玉米淀粉或葡萄糖为碳源发酵生产黄原胶,从而为完善我国黄原胶生产技术提供了一条新的途径。  相似文献   

3.
黄单胞菌(Xanthomonas Campestris.X.C.)的利福平及杆菌肽抗性突变与产黄原胶(xanthan gum)的能力正相关。从出发菌株X.C.NK-01中筛选自发突变的利福平抗性(rifampicin—resistent.Rif)和抗菌肽抗性(bacitracin—resistent,Baci)菌株,然后通过28℃,200r/min的250ml摇瓶发酵试验,筛选到两株Rifr及两株Bacir菌株。其产量分别比出发菌株提高了20%和30%,黄原胶粘度分别提高了20%-30%。分析了利福平和杆菌肽抗性突变菌株产胶能力提高的可能生化因素。  相似文献   

4.
黄原胶是由野油菜黄单胞菌发酵生产的重要的商业微生物多糖产品,实验通过微波诱变处理保藏菌株,筛选到一株能以甘蔗糖蜜为碳源发酵生产黄原胶的优良正突变株X12,实验结果表明:在初步确定的实验条件下,该菌株发酵生产黄原胶的产量达22.14 g/L,是未诱变菌株20183的1.53倍,传代实验表明,突变菌株X12的黄原胶产量及性状无大变化,遗传性状稳定。  相似文献   

5.
缩酮丙酮酸转移酶基因在野油菜单胞菌中的表达   总被引:4,自引:0,他引:4  
将野油菜黄单胞菌(Xanthomonas campestris)NRRL-B1459菌株的缩酮丙酮酸转移酶基因,在pRK2073辅助质粒的协助下,经三亲本接合法,导入野油菜黄单胞菌NK01小菌落的抗性突变株(NK01·S·1)中,测定各接合子的产胶率、黄原胶粘度及丙酮的含量,得到一系列优于原始菌株的接合子,其中四株的产胶率、丙酮酸含量及黄原胶粘度分别较出发菌株提高7.28 ̄10.60%、40.24  相似文献   

6.
白蚁伞多糖的发酵生产   总被引:3,自引:0,他引:3  
对白蚁伞在摇瓶和发酵罐中发酵产生白蚁伞多糖的动力学做了较为详细的研究。比较了几种不同培养基产生白蚁伞多糖的差异,筛选出在28℃,200r/min条件下,摇瓶产生白蚁伞多糖的最适培养基为黄豆粉葡萄糖培养基(以g/L计):黄豆粉5,葡萄糖30,酵母浸膏粉2,KH2PO41,MgSO47H2O0.5,自来水配制,自然pH值(约5.5)。发酵120h,达到最大的多糖产量0.573g/L。在NewBrunswickScientific(NBS)Bioflo-Ⅱ6.6L的发酵罐上设定28℃,600r/min,5~7L/min的通气量发酵27h,达到最大的多糖产量0.565g/L。发酵过程的pH和氨基氮变化与其它食用菌的不同。对苯酚-硫酸法测定糖含量的方法作了改进,使测定糖含量的标准曲线线性和准确性得到改善  相似文献   

7.
本文研究了外界因素对于以海藻酸钠为载体的固定化弱氧化醋单胞菌AS1.188发酵特性的影响。以产酸速率为选定依据,固定化弱氧化醋单胞菌的最佳发酵条件是:温度30℃,麦茶汁初始pH值6.5,麦茶汁浓度10°BX,凝胶量9%,在此条件下,其产挥发酸速率:0.063g/L·h,产总酸速率:0.067g/L·h。  相似文献   

8.
灵芝深层培养工艺的研究   总被引:3,自引:0,他引:3  
为了提高发酵终了时灵芝菌丝浓度以获取高等真菌多糖,对灵芝AS.5.65的深层培养工艺进行了研究。确定了最适发酵条件为:5%玉米淀粉、0.3%玉米浆、1%豆饼粉、0.6%糖蜜、0.15%KH2PO4、0.075%MgSO4·7H2O,pH5.5~6.0,温度28℃,摇床转速150r/min,摇瓶种子培养3d接种。接种量5%、装液量50mL,培养5~6d可终止发酵。最终菌体干重可达27.5g/L、含粗多糖2.6g/L。  相似文献   

9.
将野油菜黄单胞菌(Xanthomonascampestris)NRRL-B-1459菌株的缩酮丙酮酸转移酶基因,在pRK2073辅助质粒的协助下,经三亲本接合法,导入野油菜黄单胞菌NK01小菌落的抗性突变株(NK01·S·1)中,测定各接合子的产胶率、黄原胶粘度及丙酮酸含量,得到一系列优于原始菌株的接合子,其中四株的产胶率、丙酮酸含量及黄原胶粘度分别较出发菌株提高7.28~10.60%、40.24~41.42%和10.79~22.30%.  相似文献   

10.
从菊芋根际土壤中分离出一株菊粉酶(InulinaseE.C.3.2.1.7)产生菌,经物理和化学诱变得高酶活黑曲霉突变株AJ1958.该菌株在培养基(0.07g/mL菊芋提取液1000mL,豆饼粉5g,麸皮5g,KCI0.7g,FeSO4·7H2O0.01g,MgSO4·7H2O0.5g,PH5.0)中,于30℃振荡培养3d,酶活力可达64μmol·min-1.酶水解反应的最适PH为3.0~4.0,最适温度为60℃,在PH2.5~5.5的范围内和75℃以下较稳定.适宜条件下,10h内0.05g/mL的菊粉溶液中之底物几乎100%被酶水解.产物糖中果糖质量分数为93%,葡萄糖质量分数为5.6%.该突变株产生的菊粉酶具有较满意的水解性和热稳定性.  相似文献   

11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.  相似文献   

12.
何延凌 《科技信息》2008,(4):258-258
Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women's language from these perspectives: pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women's language, more attention should be paid to language use in social context. What's more, the linguistic phenomena in a speaking community can be understood more thoroughly.  相似文献   

13.
There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.  相似文献   

14.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。  相似文献   

15.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

16.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.  相似文献   

17.
王慧 《科技信息》2008,(10):240-240
Wuthering Heights, Emily Bronte's only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.  相似文献   

18.
The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.  相似文献   

19.
In the 19th century the society was controlled by men, and women were just appendants of them, they had not any rights and freedom. But Jane was an exception, she showed some characteristics of early feminist. Jane showed her characteristics of feminism in three aspects: rebellion, equality, and independence. These characteristics were helpful to her success, and feminism is the only way out for women of that time.  相似文献   

20.
The non-orthogonal localized molecular orbital(NOLMO)is the most localized representation of electronic degrees of freedom.As such,NOLMOs are thus potentially the most efficient for linear scaling calculations of electronic structures for large systems.However,direct ab initio calculations with NOLMO have not been fully implemented and widely used,partly because of the slow convergence issue in the optimization of NOLMO.We devel-  相似文献   

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