共查询到10条相似文献,搜索用时 187 毫秒
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Shuai Chen Nan Qu Shouyun Cao Bauwe Hermann Shouyi Chen Wenzhong Tian Chengcai Chu 《科学通报(英文版)》2001,46(19):1635-1638
ThegdcsP promoter isolated from C3-C4 intermediate plantFlaveria anomala was fused to the β-glucuronidase (GUS) gene. The chimeric gene was inserted into the binary vector pBin19 and introduced
into the rice (Oryza sativa L.) cv. 8706 byAgrobacteriummediated gene transfer. GUS activity can be detected in leaf, leaf sheath, stem and root tissues via fluorometric GUS assay. However,
no GUS activity was found in mature endosperm. Histochemical localization revealed that GUS expression was exclusively restricted
to vascular tissues in transgenic plants. This promoter also showed spatial-temporal expression patterns that GUS expression
declined significantly with the maturity of plants. These expression patterns make thegdcsP promoter extremely valuable in the applied biotechnology that needs target gene expression restricted to vascular tissues. 相似文献
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Xiuhua Chen Qiaoquan Liu Zongyang Wang Xingwen Wang Xiuling Cai Jingliu Zhang Minghong Gu 《科学通报(英文版)》2002,47(14):1192-1197
Amylose content in rice endosperm is one of the key determinants of rice eating and cooking quality, and the poor quality
ofindica hybrid rice is closely related to the high amylose level in rice grains. In order to improve the grain quality of theindica hybrid rice by genetic engineering, an antisense fragment of ricewaxy gene, driven by the 5′-franking sequences of the ricewaxy gene, was successfully introduced into three major parent lines ofindica hybrid rice, all contain a high amylose level in the grains, viaAgrobacterium, and more than 100 hygromycinresistant plants were regenerated. The analysis of PCR amplification and Southern blots indicated
that the T-DNA containing the antisensewaxy gene had been integrated into the genome of transgenic rice plants. Most of the primary transgenic rice plants grew normally,
and the mature seeds from these transgenic plants were performed for analysis of the amylose content. The results showed that
the amylose content in the endosperm of some grains was reduced and the lowest reached 7.02% in one homozygous transgenic
line, 72.4% lower than that of the wild type. The influence of the altered amylose content on the gelatinization temperature
and gel consistency was also observed in several homozygous transgenic rice plants.
The two authors contributed equally to this work. 相似文献
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Based on the published sequence of profilin2 promoter ofArabidopsis thaliana, a full-length promoter (1667 bp) was amplified by PCR. The 5′-end deletion fragments with length of 1380, 1153, 969 and
597 bp were then fused withgus (uidA) gene respectively. Constructed plant expression vectors were individually transferred intoKalanchoe laciniata and transgenic plants regenerated. GUS histochemical assay confirmed that the full-length promoter Pfn1.7 was vascular-specific.
Deletion assays showed that profilin2 promoter could be divided into three parts. Deletion of fragment 1 (−1667—−1380 bp)
resulted in constitutive expression, suggesting that element(s) responsible for vascular-specific expression might exist in
this region. Fragment 2 located at −1153—−597 bp strongly inhibitedgus gene expression. Fragment 3 (−597—−1 bp) is considered as a basic domain of profilin2. 相似文献
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Resistance to rice blast (Pyricularia oryzae) caused by the expression of trichosanthin gene in transgenic rice plants transferred through agrobacterium method 总被引:11,自引:0,他引:11
Fig.. Growth of transgenic plantswith trichosanthin GUS gene weeks after inoculation of Pyricularia oryzae. Transgenic rice plants with pCambia; transgenic rice plants with pC-HY. 《科学通报(英文版)》2000,45(19):1774-1778
The gene of trichosanthin has been transferred into rice plants through agrobacterium method. The single copy insertion and
the expression of foreign gene have been proved in regenerated plants. In antifungal assay the degrees of rice blast (Pyricularia oryzae) infection of the transgenic plants expressing trichosanthin and expressingGUS gene as control have been evaluated. The differences such as the time of disease symptom observed, the number of infected
plants and damaged leaves, the growth of infected plants of the two transgenic plants after being inoculated by rice blast
(Pyricularia oryzae) are significant. The transgenic plants with trichosanthin gene grew faster than the plants withGUS gene, even when humidity environment was removed. The results show that the transgenic plants that expressed trichosanthin
are able to delay the infection of rice blast compared with the plants as control. In addition, no damage caused by the expression
of trichosanthin gene in transgenic plants has been observed. 相似文献
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HengXiu Yu Xin Wang ZhiYun Gong Ding Tang MingHong Gu ZhuKuan Cheng 《科学通报(英文版)》2008,53(19):2981-2988
In order to investigate rice functional centromeres, OsCENH3-GFP chimeric gene was constructed and transformed into the indica rice variety, Zhongxian 3037, mediated by Agrobacturium. The integration of the exogenous genes in the transgenic plants was confirmed by PCR and Southern blotting. The transgenic plants grow normally during their whole life time, just like Zhongxian 3037. No significant defects were detected in either mitosis or meiosis of the transgenic plants. The overlapping of GFP signals and anti-CENH3 foci in both mitotic and meiotic cells from T0 and T1 generation plants indicated that GFP had been successfully fused with CENH3, so the GFP signals can well represent the CENH3 locations on each chromosome. To evaluate the applicability of the transgenic plants to other genetic studies, fluorescence in situ hybridization (FISH) using rice centromeric tandem repetitive sequence CentO as the probe was conducted on the zygotene chromosomes of pollen mother cells (PMCs). It has been revealed that the GFP signals are overlapping with CentO FISH signals, showing that CentO is one of the key elements constituting rice functional centromeres. Immunofluorescent staining using anti-o-tublin antibody and anti-PAIR2 antibody on the chromosomes during mitosis and meiosis stages of the transgenic plants further reveals that OsCENH3-GFP transgenic plants can be widely used for studying rice molecular biology, especially for tagging functional centromeres in both living cells and tissues. 相似文献
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Weidong Yong Kang Chong Tiebing Liang Zhihong Xu Kehui Tan Zhiqing Zhu 《科学通报(英文版)》1999,44(14):1289-1294
Based on the cDNA fragment sequence of vernalization-related geneverc203 cloned by differential screening in our lab, the 5′ primer has been designed. The cDNA 3′ end ofver203 gene (1 197 bp) has been cloned by the RACE method. And it is identified by Northern blotting that its expression is special
in vernalization treatment. After comparing the sequence in the nucleotide sequence databases of Genbank, EMBL and DDBJ, the
gene has homology withHordeum vulgare jesmonate-induced protein gene. It is suggested that this gene might be related to the signal transduction mediated by jamonate. 相似文献
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Overexpression of the yeastHAL2 gene increases salt tolerance of yeast and plant. RiceHAL2-like (RHL) gene was introduced into ajaponica rice cultivar HJ19 withAgrobacterium tumefaciens-mediated transformation. Transgenic plants in R0 generation were selected on the principle of GUS-positive,RHL gene PCR-positive and normal growth. Hygromycin-resistant plants of some transgenic lines in R1 generation increased salt tolerance during the seedling and booting stage, being less damaged in the cytomembrane and stronger
in leaf tissue viability under salt stress during booting period. Southern analysis of transgenic lines tolerant to salt in
R1 generation showed that theRHL gene expression cassette had been successfully integrated into rice genome. Moreover, gene engineering breeding methodology
and really salt-tolerant rice cultivar were discussed. 相似文献