细胞小片规格对三角帆蚌内脏团培育圆形有核珍珠质量的影响

报导了在三角帆蚌内脏团培育圆形有核珍珠中细胞小片规格与珠核规格之间的匹配试验结果。对有核珍珠从成珠率、尾巴珠率、光洁度、光泽度、正圆率和珍珠等级的影响比较表明,珠核直径在8.00～8.25 mm时,以2.0 mm×2.0 mm的小片规格为宜,有利于优质有核珍珠的培育。     

正圆形珠核规格与育珠蚌体长的匹配试验

报导了在三角帆蚌内脏团植入正圆形珠核与蚌体长之间的匹配实验结果。比较了不同规格的蚌体对有核珍珠留核率、成珠率、尾巴珠率、光洁度、光泽度、正圆度及珍珠等级的影响。实验结果表明,对于8.00～8.25 mm的珠核而言,植入12.0～12.2 cm的蚌体的内脏团效果比较理想。     

蚕豆根尖微核试验研究云南松松塔球提取物抑突变性

目的:探讨云南松松塔球提取物的抑突变作用。方法:分别将大理邓川和湾桥产的蚕豆随机分为4组,经实验处理后,采用蚕豆根尖微核技术对蚕豆根尖细胞微核数进行检测,考察松塔球提取物对蚕豆根尖细胞微核率的影响情况。结果:松塔球提取物I对邓川产蚕豆根尖细胞微核的产生有抑制作用,抑制率达50%,且对环磷酰胺诱导的蚕豆根尖微核有明显的抑制作用,抑制率为62.5%。松塔球提取物I对湾桥产蚕豆根尖细胞微核的产生有抑制作用,抑制率达50%,且对环磷酰胺诱导的蚕豆根尖细胞微核有明显的抑制作用,抑制率为50%。结论:松塔球提取物可有效抑制环磷酰胺诱导的蚕豆根尖微核的产生,具有抑突变性,从而说明松塔球提取物对肿瘤的防治可能具有积极的作用。     

大豆开花期缺水和遮光处理叶片解剖结构的观察

大豆珍珠塔3号具有双子叶植物叶片结构特征,但在海绵组织最上层有一层特殊细胞——平脉叶肉细胞,此层细胞具有水平运输和气体交换的特殊作用。大豆开花期光照对叶片结构发育起主导作用;水分亦是影响叶片结构发育的重要因素;光弱块水条件下,大豆表现出对光的竞争比对水的竞争更明显。     

马氏珠母贝珍珠囊体外培养及插囊育珠

为建立马氏珠母贝珍珠囊体外培养的插囊育珠技术,一用０．３％胰蛋白酶消化液在ｐＨ值７．２￣７．４和温度２５℃条件下对马氏珠母贝外套膜组织进行解离,收集消化１５ｍｉｎ的细胞悬液于珠核上培养珍珠囊;二直接用外套膜组织块在珠核上形成珍珠囊。方法一所产生的珍珠囊未培育出珍珠;方法二所产生的珍珠囊培育出少量的珍珠。为促进细胞在珠核上的粘附和珍珠囊的形成,研究了各种贴壁因子的作用,其中多聚赖氨酸和ＳＭ物质能明显     

脱脂棉替代尼龙毛分离T细胞用于免疫学实验教学的探讨

探讨脱脂棉和尼龙毛分离纯化外周血T淋巴细胞的方法和特点,建立脱脂棉分离T细胞的方法.采用密度梯度离心法分离人外周血单个核细胞,分别以脱脂棉和尼龙毛分离出T细胞,使用流式细胞仪鉴定细胞纯度和回收率,台盼蓝检测细胞活力.脱脂棉分离T细胞的纯度、回收率和存活率虽然都略低于尼龙毛分离法,但两者比较均无显著性差异(p＞0.05).结果显示脱脂棉分离T细胞是一种简易、快速、经济、纯度较高和基本上不损伤细胞活性的方法,除了可以代替尼龙毛用于免疫学实验教学中,也可作为开放实验培养学生的创新能力.     

日本的珍珠增产技术

近几年来日本在提高珍珠产量与质量的研究,取得了一些引人注目的成果。其中效果较为显著的有珍珠贝养殖磁性笼及“ELEX”养殖水槽的开发与应用,插核用外套膜细胞小片赋活剂、处理液和组织培养液等的应用。1.磁性养殖笼将铁酸盐(纯粒铁)、磁铁粉末等永久磁铁粉和     

淮安市不同区域河流水质诱发蝌蚪红细胞微核及核异常的研究

采用微核试验法,对贯穿淮安城市中心、边缘、外围的3条主要河流里运河、废黄河、盐河水质诱发蝌蚪红细胞微核及核异常进行了比较研究.每条河水样又分为100%原样、50%,33.3%3个浓度组,以暴气后的自来水为对照.实验结果显示,毒染1周后,里运河、废黄河水质诱发蝌蚪红细胞微核率及核异常率均大于对照组(P<0.01),里运河水组诱发微核细胞率明显高于废黄河水组和盐河水组(P<0.01).而盐河水组诱发微核率及核异常率(33.3%浓度组)与对照组之间差异不显著(P>0.05).实验结果提示,淮安城区中心地段河流水质诱发蝌蚪红细胞微核率的影响明显高于外围,其水体中存在致突变物质.     

利用蚕豆根尖细胞微核监测滇池水质污染的初步研究

本文在利用蚕豆根尖细胞微核监测滇池水质方面作了一些初步研究。以蒸馏水处理作阴性对照试验,以不同浓度的Cd,Hg处理作阳性对照试验,用蚕豆根尖细胞微核对滇池11个样点的水样进行了监测评价。结果表明:①水体Hg,Cd污染均会明显地增加蚕豆根尖细胞微核率,而且Hg对蚕豆根尖细胞微核率的影响较大。②滇池11个样点的水样均使蚕豆根尖细胞微核率明显地增加,而且,各样点水样对蚕豆根尖细胞微核率的影响不相同。③以蚕豆根尖细胞微核率为指标,监测滇池水质污染的结果与水质测定结果是一致的。④本试验可将11个样点的水质污染分为三种程度,即重污染、中污染和轻污染。     

载荷bFGF-PLGA微球的胶原海绵的制备及其体外缓释性能研究

在支架材料上引入具有控释行为的生长因子旨在结构与功能上模拟细胞外基质能.制备了一种载荷bFGF-PLGA微球的胶原海绵缓释体系,探讨其在体外对bFGF的释放性能.采用复乳溶剂挥发法制作微球,将微球与胶原海绵复合;通过扫描电镜观察微球和载荷微球胶原海绵的表面形态结构;利用ELISA法测试微球中药物的载药量和包封率,并对微球和载荷微球胶原海绵中bFGF的体外释放行为进行研究.结果表明:微球表面圆滑,载药量为0.059 9%±0.001 9%,包封率为79.9%±2.8%;载荷微球海绵的突释率为14.6%,低于微球本身20.0%的水平;而且载荷微球海绵的体外释放bFGF时间比微球本身更长.载荷bFGF-PLGA微球的胶原海绵缓释体系在体外能够稳定地缓释bFGF,具有较小的突释率,有望发展成为一种理想的组织工程支架材料.     

育珠蚌外套膜组织培养适宜条件的研究

对育珠蚌外套膜细胞在组织培养过程中分泌珠质的适宜条件进行了研究。经过几年的实验和比较,筛选出了适宜于河蚌外套膜细胞生长、分泌的培养基及一套完善的操作方法,能使育珠蚌外套膜的细胞在体外人工培养条件下较旺盛地生长、繁殖、分泌,并产生大量的珍珠质.     

Cloning and characterization of 37 kD laminin receptor precursor in pearl oyster, Pinctada fucata

A 1063 bp cDNA clone encoding a putative 37 kDa laminin receptor precursor （37 kDa LRP） is isolated from the mantle tissue of pearl oyster, Pinctadafucata. The amino acid sequence predicted from the cDNA sequence is 301 residues long, with a calculated molecular mass of 33.5 kDa. RT-PCR analysis shows that 37 kDa LRP mRNA is especially highly expressed in the mantle while widely expressed in several tissues. In situ hybridization analysis reveals that 37 kDa LRP is expressed in the outer epithelial ceils of the mantle edge, suggesting its involvement in cell proliferation and secretion in P. fucata. The identification and characterization of 37 kDa LRP in the pearl oyster will help us to further understand the signal transduction in the processes of mantle epithelial cell proliferation and tissue formation.     

Cloning and characterization of 37 kDa laminin receptor precursor in pearl oyster, Pinctada fucata

A 1063 bp cDNA clone encoding a putative 37 kDa laminin receptor precursor (37 kDa LRP) is isolated from the mantle tissue of pearl oyster, Pinctadafucata. The amino acid sequence predicted from the cDNA sequence is 301 residues long, with a calculated molecular mass of 33.5 kDa. RT-PCR analysis shows that 37 kDa LRP mRNA is especially highly expressed in the mantle while widely expressed in several tissues. In situ hybridization analysis reveals that 37 kDa LRP is expressed in the outer epithelial cells of the mantle edge, suggesting its involvement in cell proliferation and secretion in P. Fucata. The identification and characterization of 37 kDa LRP in the pearl oyster will help us to further understand the signal transduction in the processes of mantle epithelial cell proliferation and tissue formation.     

Cloning and characterization of 37 kD laminin receptor precursor in pearl oyster, Pinctada fucata

A 1063bp cDNA clone encoding a putative 37 kD laminin receptor precursor (37 kD LRP) is isolated from the mantle tissue of pearl oyster, Pinctada fucata. The amino acid sequence predicted from the cDNA sequence is 301 residues long, with a calculated molecular mass of 33.5 kD. RT-PCR analysis shows that 37 kD LRP mRNA is especially highly expressed in the mantle while widely expressed in several tissues. In situ hybridization analysis reveales that 37 kD LRP is expressed in the outer epithelial cells of the mantle edge, suggesting its involvement in cell proliferation and secretion in P. fucata. The identification and characterization of 37 kD LRP in the pearl oyster will help us to further understand the signal transduction in the processes of mantle epithelial cell proliferation and tissue formation.     

育珠河蚌分泌珍珠质的外表皮细胞的扫描电镜观察

用石蜡切片和酶消化的育珠河蚌外套膜的外表皮细胞,作了扫描电镜观察,发现有4类表面结构不同的细胞。细胞间有细丝相连结。研究表明,外表皮细胞除了有分泌珍珠质的功能外,还有物质吸收、保护的功能。     

背角无齿蚌组织培养细胞分泌的珍珠质的药理作用研究

利用背角无齿蚌的外套膜,进行离体组织培养,所得培养液及组织水解液,作用于大鼠离体子宫、兔离体小肠,其药理作用与珍珠质有效成分之一的牛磺酸相同,结果证明,背角无齿蚌外套膜培养细胞能分泌珍珠质,具有天然珍珠的药用有效成分及相同的活性.     

Effects on the early development of the pearl sac by cytochrome c, yolk lecithin and polyvinyplyrro

Mantle pieces ofHyriopisis cumingii was treated by three kinds of solution, These mantle pieces were inserted together with paraffin nucleuses into the connective tissue of three groups ofHyriopisis cumingii. These operated animals were cultured in a pool for a month. Several pearl sacs were put out and immersed by Bouin's solution after every five days. Sections of each animals were made by histological method. Those without treatment were in a control group. Observations of these sections showed that cytochrome c and yolk lecithin have an accelerated roles on pearl sac development and pearl layer secretion. No effects on pearl sac development by polyvinyplyrrolidone (PVP). Zheng Junying:born in May, 1966, Lecturer     

海水珍珠分泌细胞在白云岩表面的沉积序列

采用“平板珍珠”研究方法,将白云岩平板插入和固定在马氏珠母贝Pinctadamartcnsij（Dunkcr）的内表面,获得不同阶段的平板珍珠、采用扫描电子显徽镜观察在平板珍珠形成过程中沉积序列,旨在阐明海水珍珠的形成机理。为开发新型珠核材料提供理论指导。     

冶锌工业废渣中镉的回收利用

从冶锌工业废渣中回收金属镉，有利于提高经济效益、改善生态环境．对锌粉、锌片和锌粒置换金属镉进行了对比实验，提出了锌片置换的新方法，并对提取工艺及其条件进行了研究，制得了纯度90％以上的海绵镉．