白细胞介素—4的研究进展

IL_4是由活化的辅助性T细胞和肥大细胞产生的一种具有多种反应性免疫调控因子,且具有显著的抗肿瘤效应,其产生细胞,在人类和小鼠T细胞中似乎只育CD_~ 4细胞亚群。按其活化后分泌因子的不同可将小鼠分为T_(H1)和T_(H2)两个亚群,IL_4是由T_(H2)细胞分泌的。在人类T细胞克隆中尚未发现此类亚群,但有证据表明外周T淋巴细胞中经植物血凝素及抗CD_3单克隆抗体刺激后能产生白细胞介素—4的细胞仅限于辅助—诱导型亚群(CD_~ 4/CD_45R)IL_4编码基因     

JAK3 mediatesc-myc gene expression induced by interleukin-2

C myc gene expression can be rapidly induced by IL 2 through intracellular signal transduction which is triggered by the interaction between IL 2 and its receptor (IL 2R). JAK3 which associates to the intracellular domain of IL_2R γ may play a critical role in this process. To reveal the action of JAK3 in c myc induction, a chimeric receptor gene IL_2R α/γ/Δ NJAK3 is constructed which consists of extracellular domain derived from IL 2R α subunit (IL_2R α), the transmembrane sequence derived from IL_2R γ and the cytoplasmic domain derived from the catalytic domain of JAK3 (Δ NJAK3), and then transfected this chimeric gene into mouse fibroblast cell line L929 β which had been transfected with IL_2R β gene and stably expressed IL_2R β in high level. In the transfectants coexpressing IL_2R β and α/γ/Δ NJAK3, the stimulation of IL_2 could intensively induce c myc gene expression. Because the whole cytoplasmic domain of IL_2R γ which could recruit signaling molecules was replaced by JAK3 and the c myc could be still induced by IL_2 in this situation, the results here gave the direct evidence demonstrating that JAK3 plays an important role in c myc gene expression induced by IL_2.     

SO(8)模型中对一对与四极一四极相互作用

使用对一对与四极一四极相互作用,描述振动到γ—不稳定区域的原子核。这个相互作用有三种极限:su(2) so(5)、so(7)和so(6)极限。同位素~(98-104)Ru位于so(7)—so(6)区域。     

基于液芯柱透镜测量随浓度变化的液相扩散系数：方法综述

液相扩散系数是溶液浓度的函数D(c)，通常采用浓度配对法、Boltzmann-Matano(BM)图解法、Hall解析法和多项式数值逼近等方法测量D(c)关联式.文章在综述以上几种测量方法的基础上，重点介绍了测量D(c)关联式的一种新方法，即BM半解析法. BM半解析法基于液芯柱透镜测量扩散过程中溶液浓度的空时分布函数cexpt(x, t)s，结合Hall解析法以及BM图解法，能够快速、准确、稳定地测量D(c)关联式.用BM半解析法测量了葡萄糖水溶液在室温下的D(c)关联式，采用时域有限差分法(FDTD)求解Fick扩散方程，计算了不同D(c)关联式下扩散浓度的空时分布函数，计算结果与实验浓度分布函数cexpt(x, t)s进行了分析比较.结果表明，采用FDTD计算并比较实验扩散浓度空时分布的方法，为实测D(c)关联式的正确性提供了一种有效的判断手段.     

Azo dye degradation behavior of AlFeMnTiM(M = Cr,Co, Ni) high-entropy alloys

Because of the potential carcinogenic effects and difficult degradation of azo dyes, their degradation has been a longstanding problem. The degradation of azo dye Direct Blue 6(DB6) using ball-milled(BM) high-entropy alloy(HEA) powders was characterized in this work. Newly designed AlFeMnTiM(M = Cr, Co, Ni) HEAs synthesized by mechanical alloying(MA) showed excellent performance in the degradation of azo dye DB6. The degradation efficiency of AlFeMnTiCr is approximately 19 times greater than that of the widely used commercial Fe–Si–B amorphous alloy ribbons and more than 100 times greater than that of the widely used commercial zero-valent iron(ZVI) powders. The galvanic-cell effect and the unique crystal structure are responsible for the good degradation performance of the BM HEAs. This study indicates that BM HEAs are attractive, valuable, and promising environmental catalysts for wastewater contaminated by azo dyes.     

Azo dye degradation behavior of AlFeMnTiM (M=Cr,Co, Ni) high-entropy alloys

Because of the potential carcinogenic effects and difficult degradation of azo dyes, their degradation has been a longstanding problem. The degradation of azo dye Direct Blue 6(DB6) using ball-milled(BM) high-entropy alloy(HEA) powders was characterized in this work. Newly designed AlFeMnTiM(M = Cr, Co, Ni) HEAs synthesized by mechanical alloying(MA) showed excellent performance in the degradation of azo dye DB6. The degradation efficiency of AlFeMnTiCr is approximately 19 times greater than that of the widely used commercial Fe–Si–B amorphous alloy ribbons and more than 100 times greater than that of the widely used commercial zero-valent iron(ZVI) powders. The galvanic-cell effect and the unique crystal structure are responsible for the good degradation performance of the BM HEAs. This study indicates that BM HEAs are attractive, valuable, and promising environmental catalysts for wastewater contaminated by azo dyes.     

红丝线草对哇巴因所致豚鼠心律失常及LD100的影响

本研究主要探讨红丝线草的醇提水转溶物(简称01—w)对哇巴因致豚鼠心律失常和LD_(100)的影响。给药组采用三种不同浓度的01—w并分别与0.0125％的哇巴因同速(0.034ml/min)经豚鼠双侧颈外静脉灌注,对照组单用哇巴因,其浓度及灌注速度同给药组。实验结果表明:0.2g(生药)/ml组能显著提高哇巴因致豚鼠心律失常和LD_(10)的剂量(P<0.01),其它剂量组虽略能提高哇巴因致豚鼠心律失常及LD_(100)的剂量,但无统计学显著性。关于ol—w对抗强心甙毒性作用的机制正在深入研究中,可能与它的心肌保护作用有关。     

青海沙蜥的生长及两性生长差异

为了研究自然生境中沙蜥的生长和两性生长差异情况，于2001～2002两年间对分布在四川西北部若尔盖地区的青海沙蜥（Phrynocephalus vlangalii）进行了6次标志重捕．结果表明：第一年8、9月出生的沙蜥到第二年的6月SVL、TL和BM都有显著的生长（P〈0、05），雌、雄个体的两性生长差异不显著（P〉0．05）；第二年6月至8、9月间，雌、雄个体的SV、TL和BM都有快速的生长（P〈0．01），从7月份开始出现了两性生长差异（雌性的生长速度小于雄性）（P〈0．01）；第三年的6月至8月间，雌性的SVL、TL和BM仍在快速生长（P〈0．01），而雄性的生长则不显著（P〉0．05），雌性的三个生长指标的生长量远大于雄性（P〈0．01），并且SVL和BM的生长量与第二年的同期生长量非常接近（P〉0．05）；四年以后，雌、雄个体的SVL和TL的生长及两性生长差异都不再显著（P〉0．05），但是在体形大小上还存在极显著的两性异形（P〈0．01）．在青海沙蜥的生长发育过程中，雄性的生长曲线近似“S”形，而雌性则是非“S”形．雌、雄个体的生长受季节和年龄的影响，造成雌、雄两性成长差异的主要原因是雌、雄个体对繁殖的能量投入不平衡．     

Up-regulation of endothelial nitric oxide synthase by cytochrome P450 arachidonic acid epoxygenase BM3F87V

Cytochrome P450 (CYP)-dependent metabolites of arachidonic acid, epoxyeicosatrienoic acids (EETs), have been suggested to be an endothelium-derived hyperpolarizing factor (EDHF). However, the interaction or relation between EDHF and endothelial nitric oxide synthase (eNOS) is still to be elucidated. In the present study, the regulation of eNOS by endogenous EDHF is examined. The cytochrome P450 epoxygenase BM3F87V is cloned into the mammalian expression vector pCB6. Cultured bovine aortic endothelial cells (BAECs) less than 4 passages are used and transfected with BM3F87V. The effects of endogenous EETs result from BM3F87V transfection on eNOS are assessed in the endothelial cells by Western blot and Northern blot, and eNOS activity is also measured by the conversion of L-arginine to L-citrulline. Compared to transfection with the empty pCB6 vector, transfection of BAECs with BM3F87V significantly elevates the levels of eNOS protein expression, which is markedly inhibited by treatment with CYP inhibitor 17-ODYA. BM3F87V transfection also elevates the eNOS mRNA level and increases the eNOS activity. This study suggests that EDHF up-regulates eNOS gene expression.     

植物生长调节剂诱导龙眼果实无核化效应

对在福建主栽的大核龙眼品种‘乌龙岭'进行了植物生长调节剂赤霉素(GA3)、6-苄氨基嘌呤(6-BA)、2,4,5-三氯苯氧丙酸(2,4,5-TP)、多效唑(PP333)和青鲜素(MH)等诱导果实无核化的试验.结果表明:5种植物生长调节剂均在不同程度上诱导龙眼果实形成焦核和小核果;6-BA和MH是效果较好的龙眼果实无核化诱导剂;效果最好的两个处理分别为6-BA(100 mg/L) CPPU(30 mg/L)和MH(800 mg/L) CPPU(30 mg/L),其果实核重分别比对照减少38.5%和38.0%,单果重分别为9.77 g和8.42 g,可溶性固形物分别为18.57%和18.23%,小核率分别达62.2%和83.3%.     

一株产普鲁兰酶细菌的分离鉴定及其发酵条件优化

从淀粉加工厂附近土壤中筛选得到一株普鲁兰酶高产菌株,编号为Z-13,其初始酶活达到5.7 U/mL.通过对此菌株的16S rDNA比对,以及生理生化鉴定,鉴定此菌株为克雷伯氏菌(Klebsiella variicola),通过发酵条件优化,确定最佳发酵产酶条件为:玉米淀粉1.5%,蛋白胨2.0%,KH2PO40.05%,MgSO4·7H2O 0.01%.装液量为70 L/250 L,培养基最初pH为6.0,发酵温度30℃,摇床转速200 r/min,发酵时间48 h,优化后菌株产普鲁兰酶的酶活高达67.8 U/mL,是优化前菌株产普鲁兰酶活性的11.89倍.     

关于镀锌添加剂的研究

在过去一百多年的历史中;镀锌绝大部分采用氰化工艺。本文根据我国无氰电镀的现状,结合现有碱性镀锌实际和环境保护的要求,作者于实验室,用不同物质,在多种实验条件下作了大量小槽实验,终于找到了Z-1镀锌添加剂。该添加剂可改善锌镀液的性能,并使镀层质量得到提高。一年多的生产实验表明:Z-1是一个良好的无氰镀锌添加剂品种。具有明显的社会和经济效益。     

Electrophysiology and behavior feedback of diamondback moth,Plutella xylostella, to volatile secondary metabolites emitted by Chinese cabbage

The volatiles, absorbed by Porapak Q and eluted by hexane, from Chinese cabbage, Brassica campestris chinesis (L.) Makino. var. communis Tsen et Lee, have been identified as allyl isothiocyanate, Z-3-hexenyl acetate, 3,7-dimethyl-1,3,6-octatriene, 2,5-hexanediol, Z-3-hexen-1-ol, nonanol, b-myrcene, a-pinene, E-2-hexen-1-ol, D-limonene, Z-3-hexenyl propanoate, linalool, geraniol, E-4-hexen-1-ol, Z-3-hexenyl isovalerate, a-terpinene, b-caryophyllene, 3-carene and a-caryophyllene, by their mass spectra and retention times in comparison with authentic samples. The first five chemicals are the main components. All components can elicite electroantennogram (EAG) responses of diamondback moth (DBM), Plutella xylostella, in which allyl isothiocyanate and C₆ alcohols and esters, i.e. Z-3-hexen-1-ol, E-2-hexen-1-ol, E-4-hexen-1-ol, Z-3-hexenyl isovalerate and 2,5-hexanediol elicite stronger EAG responses than other components, and EAG responses of female moths to them are slightly larger than those of male moths. However, EAG responses elicited by terpenes are smaller, and EAG responses from females to terpenes are smaller than those from males, but b-myrcene can evoke stronger EAG responses from both females and males. Volatiles from intact Chinese cabbage, allyl isothiocyanate, 2,5-hexanediol and Z-3-hexenyl isovalerate intensely cause DBM directional flying and landing in wind tunnel, in which volatiles from intact Chinese cabbage have the strongest activity, and the next is allyl isothiocyanate. Allyl isothiocyanate is more attractive to females than to males a bit. As for a-terpinene and other subordinate components, EAG and wind tunnel test make sure they have weak activity.     

An analysis of structure fitting and bioactivity between sex pheromone of cotton bollworm, Helicoverpa armigera (Hübner) and its fluorinated analogs

A study on the structure-activty relationship between （Z）-hexadec-9-enal （Z-9-16：Ald） and its analog was conducted by comparing the structures of the sex pheromone of cotton bollworm, Helicoverpa armigera （Huebner） with its fluorinated analog using computer molecular fitting. It is demonstrated that the structure of analog substituting for hydrogen atom on the terminal carbon atom is similar to Z-9-16：Ald. The EAG result showed that there is no significant difference in activities between Z-9-16：Ald and its fluorinated analog synthesized.     

ＲAG2 /IL2ＲG 双基因缺陷小鼠杂交群体的建立

摘要: 目的建立ＲAG2 /IL2ＲG 双基因缺陷的CＲG 小鼠杂交群体。方法将ＲAG2 基因缺陷小鼠与IL2ＲG 基因缺陷小鼠分别进行繁殖,选取ＲAG2 基因缺陷雄鼠ＲAG2( － / － ) 与IL2ＲG 基因缺陷雌鼠IL2ＲG( － / － ) 进行配对,培育杂交后代,通过PCＲ 扩增基因组ＲAG2 和IL2ＲG 基因进行鉴定; 应用流式细胞仪分析检测ＲAG2 /IL2ＲG 双基因缺陷小鼠外周血T 细胞( CD3 + ) 、B 细胞( CD19 + ) 和NK 细胞( CD49b + ) 含量; 并测定8—12 周龄ＲAG2 /IL2ＲG 双基因缺陷小鼠血液生理生化及主要脏器重量指标。结果成功繁育ＲAG2 基因缺陷小鼠和IL2ＲG 基因缺陷小鼠,筛选获得稳定的ＲAG2 /IL2ＲG 双基因缺陷小鼠并成功保种和扩群; 该双基因缺陷小鼠外周血淋巴细胞中T 细胞、B 细胞,NK 细胞比例显著降低( P ＜ 0. 05) ; 与相同周龄野生型C57BL/6 相比9 项血清生化指标无明显变化( P ＞ 0. 05) ; 18 项血液生理指标中红细胞( ＲBC) 和血红蛋白( HGB) 含量显著降低( P ＜ 0. 05) ; 白细胞( WBC) 、淋巴细胞数( LYMPH) 、淋巴细胞比率( LYM) 、单核细胞、中性细胞数( NEUT) 降低极显著( P ＜ 0. 01) ; 脾脏重量显著均低于野生型C57BL/6 小鼠( P ＜ 0. 05) ; 人肝肿瘤传代细胞移植于ＲAG2 /IL2ＲG 双基因缺陷小鼠后,肿瘤移植成功率和生长速率均明显高于亲本单基因缺陷小鼠( P ＜ 0. 05) ,成功获得了临床肝癌病人肿瘤组织的异种移植模型。结论 成功构建筛选出ＲAG2 /IL2ＲG 双基因缺陷小鼠杂交群体。     

依坐标H=N-Z分布的稳定核素四边形区

在坐标为S=2Z-N、H=N-Z核素图中,讨论了坐标参量的物理意义及依坐标H常数列分布的8个稳定核素四边形区.它们分别是在坐标H16～26的一个有6×6种核素的正方形区,坐标H≤16的3个斜平行四边形,坐标H26～44的3个矩形区与最上边的小梯形.这些核素分布包含了稳定区23列偶坐标H列的大部分偶偶核素.最后研究了四边形内部核素个数的分布规律.     

雄黄对MRL／lpr小鼠脾细胞分泌干扰素-γ、白细胞介素-6的影响

目的：探讨雄黄（As4S4）对MRL／lpr狼疮鼠脾脏细胞分泌干扰素-γ（IFN-γ）、自细胞介素-6（IL-6）的影响。方法：将20周龄MRL／lpr狼疮小鼠和正常C57BL／6J小鼠无菌条件下取出脾脏,制成脾脏淋巴细胞悬液,体外经植物凝集素P（PHA-P,终浓度20mg／L）、白细胞介素-2（IL-2,终浓度10^6IU／L）常规刺激48h后,随机分组进行如下实验：（1）不同浓度As4S4溶液（6．5、12．5、25、50mg／L）作用24h;（2）As4S4对MRL／lpr小鼠和C57BL／6J小鼠作用：①PBS组：空白对照;②As4S4组：25mg／L;以上各组继续培养24h,酶联免疫吸附法（ELISA）测定各组培养液IFN-γ、IL-6表达量。结果：（1）MRL／fpr狼疮鼠PBS组IFN-γ的分泌高于C57BL／6J小鼠PBS组（P〈0．01）。（2）在MRL／加狼疮鼠中,与PBS组相比,As4S4组（25mg／L）IFN-γIL-6的分泌水平下降。（3）在C57BL／6J小鼠中,PBS组和As4S4组之间IFN-γ、IL-6浓度水平无明显差异。结论：As4S4下调MRL／lpr狼疮鼠IFN-γ、IL-6的分泌,而对C57BL／6J小鼠脾细胞IFN-γ、IL-6的分泌无明显影响。     

一种新型调剖堵水剂的研制

In waterflood development, in order to prevent the high permeability layer and crack from absorbing too much water which could result in the middle and low permeability layer cannot reach the requirement of water flooding;people have taken many kinds of methods to improve the profile of water injection.On the basis of synthetically analyzing recently differ kinds of good water plugging agent at home and abroad,we have synthesized a new type grain profile control and water plugging agent Z-1 by using AM as major raw material,organic and inorganic crosslinking agent and other additives.The Z-1 has strong plugging capacify to cores and high plugging efficiency. Also,the Z-1 has low costs,good adaptability,which is suitable for inhomogeneous oil reservoir and affusion well.So Z-1 is a type of excellent water plugging agent.     

珊瑚来源群体淬灭活性产生菌的筛选与抑菌活性研究

珊瑚病原菌的群体感应（Quorum Sensing，QS）是介导珊瑚疾病发生的重要因素，抑制病原菌的群体感应可以有效抵抗病原菌的侵染作用。为解决耐药性难题并维持珊瑚礁生态系统健康，本研究以紫色杆菌（Chromobacterium violaceum）为指示菌，从三亚市鹿回头半岛三亚湾（109°28''E，18°13''N）采集的珊瑚样品中，分离筛选具有较高群体淬灭酶活性的珊瑚共附生细菌，经16S rDNA测序初步鉴定其大部分为弧菌属。进一步研究群体淬灭活性菌株对两株珊瑚致病菌溶珊瑚弧菌V545（Vibrio coralliilyticus）和溶藻弧菌Z-14（V.alginolyticus）的生长、运动及生物被膜生成的影响。结果显示，5株群体淬灭活性菌株明显抑制溶珊瑚弧菌V545及溶藻弧菌Z-14的生长，8个菌株对溶藻弧菌Z-14的运动有明显抑制作用；9个菌株可显著抑制溶珊瑚弧菌V545生物被膜形成，13个菌株可显著抑制溶藻弧菌Z-14的生物被膜形成，其中11个菌株对溶藻弧菌Z-14生物被膜生长抑制率均超过85%；部分群体淬灭活性菌株对溶珊瑚弧菌V545和溶藻弧菌Z-14的生长、运动及生物被膜形成均有显著抑制作用。本研究筛选获得具有群体淬灭活性的细菌，并揭示其群体淬灭活性对珊瑚致病菌生长、运动、生物膜生成的影响，为解决珊瑚微生物抗病机制提供理论依据与原创材料。     

可编程和可记忆的炉温实时控制系统

本系统通过Z-80CPU中央微处理器及A/D、CTC等外围接口电路,利用热电偶进行温度检测,由毫伏放大环节及A/D转换器输入采样信号。微处理器将采样信号与给定温升曲线相比较,经过运算对炉温进行跟随控制,并随时显示现场炉温。 一、硬件设计 该系统灵活地利用具有定时和计数功能的CTC芯片来控制晶闸管导通角及采样周期,以直接产生晶闸管的触发脉冲信号,此外,对热电偶的微弱信号处理不是采用传统的温度变送器,而是利用精密运算放大器构成放大环节,以硬件处理方式将8位A/D转换精度