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1.
用甲基纤维素(MC)将肌红蛋白(Mb)固载于热裂解石墨电极表面,制备了Mb-MC膜修饰电极.修饰膜中的Mb与电极直接传递电子,在磷酸盐缓冲溶液(PBS)中循环伏安扫描可得到一对可逆的Mb辅基血红素Fe(Ⅲ)/Fe(Ⅱ)电对氧化还原峰,式电势为-0.298V(vs SCE).式电势随PBS的pH值增加而负移且成线性关系,说明Mb的电子传递过程伴随有质子的转移,最后探讨了该修饰电极对O2,H2O2和NO的电催化性质。  相似文献   

2.
用琼脂糖(agarose)将血红蛋白(Hb)固定在热裂解石墨电极表面,制备了Hb-agaose膜修饰电极.包埋在琼脂糖中的血红蛋白与电极直接传递电子.在磷酸盐缓冲溶液中得到一对可逆的血红蛋白辅基血红素Fe(Ⅲ)/Fe(Ⅱ)电对氧化还原峰,式电势为-0.299 V(vs SCE),式电势随缓冲溶液pH值增加而负移且成线性关系,直线斜率为-44 mV/pH,说明血红蛋白的电子传递过程伴随有质子的转移.并研究了Hb-agarose膜修饰电极对H2O2的电催化性质.  相似文献   

3.
用紫外-可见和红外光谱及原子力显微法表征了血红素蛋白质-甲基纤维素膜修饰电极。紫外-可见和红外光谱显示,在甲基纤维素(MC)凝胶中,蛋白质保持原始构象;在溶液pH3.0—10.0范围内,蛋白质的构象可逆地变化。原子力显微图像表明蛋白质与MC水凝胶之间存在较强的作用。研究了血红素蛋白质催化还原O2、H2O2和NO的机理。实验表明,还原O2和NO时,血红素蛋白质-MC修饰电极能重复使用,催化活性几乎不变;而催化H2O2时,催化活性下降较快,表明其反应历程有显的差异。稳定的蛋白质-MC修饰电极能定量测定H2O2和NO2^-。  相似文献   

4.
用海藻酸钠(Sodium Alginate,SA)将肌红蛋白(Mb)固定在热裂解石墨电极表面,制备了Mb.SA膜修饰电极.包埋在SA膜中的Mb在磷酸盐缓冲溶液(PBS)和乙醇混合溶液中与电极直接传递电子,得到一对对称的Mb辅基血红素Fe(Ⅲ)/Fe(Ⅱ)电对的氧化还原峰,式电势为-0.339V(vs SCE).式电势随PBSpH值增加而负移且成线性关系,直线斜率为-47.0mV/pH,说明肌红蛋白的电子传递过程伴随有质子的转移.并研究了Mb-SA膜修饰电极在PBS和乙醇混合溶液中催化还原H2O2和催化六氯乙烷脱氯,该修饰电极可用于H2O2和六氯乙烷的定量检测.  相似文献   

5.
介绍了一种简单、新颖的途径,气-液界面组装银纳米粒子成为二维纳米粒子膜.这种纳米粒子膜能有效地固定血红蛋白(Hb)到玻碳电极上.银纳米粒子膜作为电子传递的桥梁,在没有任何媒质的帮助下,能极大促进Hb和修饰的玻碳电极之间的电子传递.在pH值为7.0的磷酸盐缓冲溶液中,Hb显示一对清晰的氧化还原峰,式量电位(E0’)大约为-0.525 V(相对Ag/AgCl,KCl饱和溶液).紫外-可见光谱研究表明,固定后的Hb的生物活性保持不变.固定在银纳米粒子膜修饰的玻碳电极上的Hb可以用作生物传感器检测H2O2.当H2O2浓度从5.0μm升高到1.74 mM时,稳定的电流响应随之线性增加.H2O2的检测限(3σ)是4.3×10-7M.  相似文献   

6.
高半胱氨酸SAM膜电极的制备及其电化学行为研究   总被引:4,自引:0,他引:4  
研究了高半胱氨酸在金电极上形成单分子自组装膜的条件,并利用循环伏安法,交流阻抗谱研究了[Fe(CN)6]^3-/4-在高半胱氨酸SAM膜电极上于不同pH值溶液中的电化学行为。循环伏安结果表明,在pH大于高半胱氨酸等电点的溶液中,[Fe(CN)6]^3-/4-在膜电极上的循环伏安曲线峰电流明显降低,峰分离差增大,说明随pH值的增加,[Fe(CN)6]^3-/4-离子对在SAM膜电极上的可逆性变差;交流阻抗图谱显示,由于SAM膜电极表面带负电荷时,[Fe(CN)6]^3-/4-难以靠近电极表面,使其与电极表面的电子交换反应变得困难,在SAM膜电极上的电化学反应电阻Rct明显增加,并且随电解质溶液的pH增加而增加。  相似文献   

7.
噻吩羧酸在金表面的自组装膜及其电化学性质   总被引:1,自引:0,他引:1  
研究了具有平面环状共轭体系的硫杂环化合物噻吩羧酸在金表面的自组装单分子膜及其修饰金电极的电化学行为.以Fe(CN)6^4-/3-为“探针”离子,用循环伏安法对自组装膜的形成、电极界面电容及其组装机理进行了研究.结果是噻吩羧酸在Au上的覆盖度约为95%,金电极的界面微分充电电容由修饰前的0.294μF/cm^2下降到0.167μF/cm^2,由此可见噻吩羧酸在金表面形成了致密的单分子膜.  相似文献   

8.
三氯醋酸产生于工业废弃物及水的氯化过程,是重要的环境污染物,对于它的检测,常用的方法比较繁琐,应用血红蛋白-蒙脱土膜修饰热解石墨电极,将血红蛋白包埋于修饰在热解石墨电极上的蒙脱土内,进行循环伏安实验,可以得到血红蛋白的直接电化学响应,在pH4.00.1mol/L柠檬酸缓冲液中,于-0.292V处出现-对准可逆的氧化还原峰(相对于饱和甘汞电极,vs.SCE)经紫外-可见光谱和傅利叶红外光谱测定,包埋在蒙脱土膜内的血红蛋白仍保持与其天然状态相似的二级结构,而且将三氯醋酸加入上述体系中,发现该修饰电极对三氯醋酸具有催化功能,表观米氏常数的测定结果显示,该参数可作为这种电极与底物三氯醋酸作用的酶性质的表征,同时血红蛋白-蒙脱土膜修饰热解石墨电极性质稳定,对于三氯醋酸,显示出了酶样的催化作用特性,特异性高,因此水中虽然存在一些浓度高于三氯醋酸三倍的相关干扰物质的同时,该电极仍显示出了良好的选择性,并具有方便、快捷的特点.还从电化学的角度出发,探讨了血红蛋白-蒙脱土膜的修饰电极在环境污染物三氯醋酸检测中应用的可能性。  相似文献   

9.
应用交流阻抗技术研究了12烷基硫醇自组装膜(SAM)修饰金电极在2mmol/L Fe(CN)6^3-/Fe(CN)6^4-溶液中的电化学行为。无“针孔”缺陷的SAM修饰电极对溶液中电对的电子转移具有良好的阻碍作用,在所测定的外加直流电位下,12烷基硫醇自组装膜修饰金电极在Fe(CN)6^3-/Fe(CN)6^4-溶液中的电化学交流阻抗谱均表现为半圆形式。指出了自组装膜修饰电极在Fe(CN)6^3-/Fe(CN)6^4-溶液中的行为实质上主要反映了膜自身的电阻特征,发现表观电阻ln(1/R2)与η^1/2之间具有良好的线性关系,应用有机超薄绝缘膜的Poole-Frenkel效应对此进行了解释。  相似文献   

10.
采用3-巯基丙酸在金电极上自组装得到自组装单分子层(SAM),再利用DCC作酰胺键偶合剂,将超氧化物歧化酶(SOD)模型化合物Fe(IDB)CI3(简称Fe-MSOD,IDB为N,N-二(2’-苯并咪唑甲基)亚胺)共价键合到自组装在金电极表面的3-巯基丙酸单分子层上,构筑了一种超氧化物歧化酶模型化合物分子组装体系膜修饰电极(Fe-MSOD/3-MPA/Au).采用电化学循环伏安法、交流阻抗法对该修饰电极进行了表征.初步探讨了此修饰电极的电化学氧化还原性质.  相似文献   

11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.  相似文献   

12.
何延凌 《科技信息》2008,(4):258-258
Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women's language from these perspectives: pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women's language, more attention should be paid to language use in social context. What's more, the linguistic phenomena in a speaking community can be understood more thoroughly.  相似文献   

13.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

14.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。  相似文献   

15.
王慧 《科技信息》2008,(10):240-240
Wuthering Heights, Emily Bronte's only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.  相似文献   

16.
The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.  相似文献   

17.
There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.  相似文献   

18.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.  相似文献   

19.
In the 19th century the society was controlled by men, and women were just appendants of them, they had not any rights and freedom. But Jane was an exception, she showed some characteristics of early feminist. Jane showed her characteristics of feminism in three aspects: rebellion, equality, and independence. These characteristics were helpful to her success, and feminism is the only way out for women of that time.  相似文献   

20.
The non-orthogonal localized molecular orbital(NOLMO)is the most localized representation of electronic degrees of freedom.As such,NOLMOs are thus potentially the most efficient for linear scaling calculations of electronic structures for large systems.However,direct ab initio calculations with NOLMO have not been fully implemented and widely used,partly because of the slow convergence issue in the optimization of NOLMO.We devel-  相似文献   

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