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1.
湖北侧褶蛙与黑斑侧褶蛙同工酶的比较研究   总被引:2,自引:0,他引:2  
采用垂直板聚丙烯、酰胺凝胶电泳方法,对湖北侧褶蛙(Pelophylax hubeiensisi)和黑斑侧褶蛙(Pelophylax mgromaculata)的消化道、心脏、肌肉、脾脏、肾脏、脑、肝脏、生殖腺、晶体和肺等10种组织中的酯酶(EST)、苹果酸脱氢酶(MDH)、乙醇脱氢酶(ADH)、乳酸脱氢酶(LDH)、过氧化物酶(POX)等5种同工酶进行了比较研究。分析并讨论了5种同工酶在不同组织中的分布及遗传基础。结果显示,两种蛙的POX、MDH、ADH同工酶表现明显的遗传相似性;EST、LDH同工酶存在较大的种间差异。  相似文献   

2.
采用聚丙烯酰胺垂直板梯度凝胶电泳技术,对细角螺7种组织(外套膜、肌肉、肝脏、心脏、生殖腺和缠卵腺)的7种同工酶(EST,SOD,ME,MDH,LDH,ADH,GDH)进行了分析,结果表明:所测组织中未检出ADH和 GDH,LDH除了在肝脏中呈现较浓的条带外,在其他组织均呈现微弱条带,无组织差异性;ME,EST,SOD,MDH在组织间具有显著的差异.  相似文献   

3.
采用垂直板状聚丙烯酰胺凝胶电泳,对鸭绿江流域松江鲈鱼天然种群的8种同工酶(EST,MDH,LDH,ME,α-AMY,GDH,ADH,G6PD)的20个基因座位进行分析,结果表明,该野生种群的多态座位比例(P)为10%,平均杂合度(H)为0.030,均低于与其相似生活习性的其他鱼类的平均值,以此讨论了松江鲈鱼的种质资源状况及生态环境的关系。  相似文献   

4.
采用垂直板聚丙烯酰胺凝胶电泳方法,对沙塘鳢(Odontobutis abscura)的消化道、心脏、肌肉、脾脏、肾脏、脑、肝脏、生殖腺和晶体9种组织的4种同工酶(EST、LDH、ADH、MDH)进行了研究,讨论了这几种同工酶在沙塘鳢不同组织中的分布以及遗传基础。EST有5个基因位点,单体酶;LDH只有1个基因位点,为四聚体酶;MDH为由3个基因位点控制的二聚体酶;ADH亦为二聚体酶,但仅在肝脏中得到表达。结果表明,同工酶的表达具有组织特异性。  相似文献   

5.
不同年龄池蝶蚌(贝)与三角帆蚌同工酶的比较   总被引:6,自引:0,他引:6  
对池蝶蚌(贝)和三角帆蚌之间的4个年龄段和7种组织(心、肝、肾、鳃、闭壳肌、外套膜、性腺)中的10种同工酶(ALP、LDH、ADH、ACP、CAT、MDH、G-6-PDH、POD、EST、SOD)进行分析。结果表明,两种贝类的同工酶具有明显的年龄、组织特异性;池蝶蚌的同工酶系统较三角帆蚌的复杂,在一定程度上揭示了所检测的池蝶蚌种群的生活力和代谢能力强于三角帆蚌种群。  相似文献   

6.
江苏水域7种重要养殖鱼类的同工酶分析   总被引:1,自引:0,他引:1  
采用垂直板不连续聚丙烯酰胺凝胶电泳,研究了江苏水域7种重要养殖鱼类肌肉的数种同工酶(LDH、EST、MDH、ME、ADH、GDH和POX),对这些酶在各鱼种表达的位点与酶带分别作了分析.根据所统计的位点和酶带数目计算出6种鲤科鱼间遗传距离和相似性系数.聚类分析显示,青鱼、草鱼与鲢、鳙先分别汇聚,然后再汇聚成一个单元,团头鲂与翘嘴红鲌汇聚成一个单元,最后这两个单元汇聚在一起,这与分类学结论一致.本文还讨论了各种鱼同工酶酶谱差异、江苏水域重要养殖鱼类资源状况以及鳜和翘嘴红鲌不同种群间酶谱异同.  相似文献   

7.
采用不连续聚丙烯酰胺垂直板电泳技术,分析了花[鱼骨]鱼鳃、眼睛、肌肉3种组织中6种同工酶差异表达,并对部分同工酶位点表达及酶谱表型进行了初步分析.实验结果显示:SDH在3种组织中均未见表达;ADH仅在肌肉组织和眼睛中出现较弱的1条酶带;其它4种酶在3种组织均有较好地表达,其中EST、SOD在组织间表达不存在明显差异;而LDH、MDH同工酶在酶带数及酶活性方面有明显的组织特异性,这可能是受不同基因位点或同一基因位点不同等位基因控制的结果.在6种同工酶中共检出7个基因座位.  相似文献   

8.
赤点石斑鱼4种同工酶的组织分布及基因位点分析   总被引:12,自引:0,他引:12  
运用聚丙烯酰胺垂直梯度凝胶电泳法对赤点石斑鱼7组织(肌肉、肝脏、肾脏、心肌、脑、脾脏、性腺)的4种同工酶(EST、LDH、MDH、ME)进行了初步研究,并对4种酶的同工酶位点及酶谱表型进行了分析.结果表明:赤点石斑鱼的4种同工酶系统具有明显的组织特异性;EST由4个基因位点编码;LDH酶带多于典型的5条酶带;MDH具有线粒体型(m-MDH)和上清液型(s-MDH)两种类型;7种组织只检测到一种类型的ME.  相似文献   

9.
采用不连续聚丙烯酰胺垂直板电泳技术,分析了花鱼鳃、眼睛、肌肉3种组织中6种同工酶差异表达,并对部分同工酶位点表达及酶谱表型进行了初步分析.实验结果显示:SDH在3种组织中均未见表达;ADH仅在肌肉组织和眼睛中出现较弱的1条酶带;其它4种酶在3种组织均有较好地表达,其中EST、SOD在组织间表达不存在明显差异;而LDH、MDH同工酶在酶带数及酶活性方面有明显的组织特异性,这可能是受不同基因位点或同一基因位点不同等位基因控制的结果.在6种同工酶中共检出7个基因座位.  相似文献   

10.
山东日照和福建厦门沿海花鲈同工酶的遗传变异分析   总被引:4,自引:0,他引:4  
利用聚丙烯酰胺凝胶电泳技术,对分别来自山东日照和福建厦门两自然群体的沿海花鲈,分析了酶表达活性强的肝脏、肌肉两组织中LDH、MDH、ME、α—GPDH、ADH、SDH、SOD、G—6PDH、EST、GDH等10种同工酶,检出19个基因座位。数据处理结果:花鲈两群体的遗传相似度和遗传距离分别为0.9902和0.0098,平均杂合度分别为0.1112及0.0939。Me—1、G—6—pdh—2、Est—1、Est—5的4个基因座位具有多态性,多态座位比例均为21.1%,座位平均有效基因数均为1.2630由此可表明日照花鲈群体的遗传多样性水平比厦门花鲈群体高。  相似文献   

11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.  相似文献   

12.
何延凌 《科技信息》2008,(4):258-258
Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women's language from these perspectives: pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women's language, more attention should be paid to language use in social context. What's more, the linguistic phenomena in a speaking community can be understood more thoroughly.  相似文献   

13.
There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.  相似文献   

14.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。  相似文献   

15.
王慧 《科技信息》2008,(10):240-240
Wuthering Heights, Emily Bronte's only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.  相似文献   

16.
The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.  相似文献   

17.
In the 19th century the society was controlled by men, and women were just appendants of them, they had not any rights and freedom. But Jane was an exception, she showed some characteristics of early feminist. Jane showed her characteristics of feminism in three aspects: rebellion, equality, and independence. These characteristics were helpful to her success, and feminism is the only way out for women of that time.  相似文献   

18.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.  相似文献   

19.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

20.
1 Rise of studies on climate change's effects on biodiversity
Until the 1980s, climate change and biodiversity were studied as two independent disciplines for more than a century. In 1992, the Ecological Society of America's annual report named climate change, biodiversity, and the sustainable ecological system as the three major global environmental issues of the twenty-first century [1].  相似文献   

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