施锌对薄壳山核桃幼苗生长及体内锌分配的影响

以薄壳山核桃1年生实生苗为材料,研究了施锌对薄壳山核桃幼苗生长的影响及锌元素在植株各器官中的分配规律。结果表明:叶面喷施低质量分数ZnSO4促进了薄壳山核桃幼苗的生长,ZnSO4质量分数过高时幼苗的生长反而受到抑制;用ZnSO4浸种也表现出"低促高抑"的效应;两种施锌方式下,随着ZnSO4质量分数的升高,叶片叶绿素含量均呈现出"下降—增加—迅速下降"的趋势;经叶面喷施后锌在薄壳山核桃各器官中的比例大幅增加,其中以叶片富集锌的量最多,茎次之,根系最少;而ZnSO4浸种后,以茎中锌积累的量最多,叶片次之,根系最少。综合试验结果认为,以0.4%ZnSO4叶面喷施对促进薄壳山核桃幼苗营养生长及光合作用效果最佳。     

青龙衣多糖对S180小鼠红细胞膜组分的影响

考察青龙衣多糖对S180小鼠红细胞膜组分及流动性的影响.青龙衣多糖对S180小鼠腹腔注射给药7 d,应用聚丙烯酰胺凝胶电泳测定S180小鼠红细胞膜带3蛋白含量,比色法结合试剂盒测定S180小鼠红细胞膜磷脂、胆固醇和唾液酸含量,荧光偏振法结合探针1,6-二苯-1、3、5己三烯测定S180小鼠红细胞膜流动性.青龙衣多糖能增加S180小鼠红细胞膜表面带3蛋白含量(P<0.05);降低S180小鼠红细胞膜Ch/PL值(P<0.05);增加S180小鼠红细胞膜表面唾液酸含量 (P<0.05);提高S180小鼠红细胞膜流动性.青龙衣多糖可以改善S180小鼠红细胞膜组分的异常变化,从而保持细胞膜的流动性,在一定程度上恢复红细胞的结构和功能.     

金催化合成硫化锌纳米线及其发光性质的研究

采用金催化和直接蒸发ZnS粉末的方法,合成出大量具有纤锌矿结构的单晶ZnS纳米线。该纳米线的线径均匀,线形规则,直径在80~120 nm,长度约几十微米。研究发现纳米线的形貌对合成的温度很敏感,合成温度的升高会导致纳米线直径的迅速增加。单根纳米线EDS分析表明,ZnS纳米线线体中均匀分布着Au元素,Au元素的掺入是纳米线生长形成后由端部颗粒通过固态扩散进入纳米线中。室温光致发光谱显示:ZnS纳米线有两个发光峰,分别位于446 nm和520 nm处。446 nm的发光峰是由缺陷所致,而520 nm左右的发光峰是由Au元素掺杂所致。     

废旧锌锰电池的回收利用

分析了我国废旧电池的回收利用现状。阐述了废旧电池对生态环境造成的危害和回收利用废旧电池的重大意义。介绍了回收利用废旧锌锰电池的工艺流程和实验原理。在现有的实验条件下,初步研究了利用废旧锌锰电池制备高锰酸钾、氯化锌、氯化铵等物质的实验方法;分析讨论了制备高锰酸钾的各种影响因素,如反应物的量、反应温度等。     

银杏叶多糖对人脐静脉内皮细胞与HL-60细胞粘附的影响

根据人脐静脉血管内皮细胞(ECV-304)的显微和超微结构,研究了银杏叶多糖(GBLP)对ECV-304细胞和HL-60细胞粘附作用的影响.结果表明,正常生长的ECV-304细胞呈不规则形,细胞质丰富,细胞核呈圆形或椭圆形.电镜下,可见细胞表面有绒毛,细胞内具有Weibel-Palade小体.当ECV-304细胞受凝血酶(thrombin)刺激后,与HL-60细胞的粘附能力明显提高;而当用200 μg/ mL和500 μg/ mL剂量的GBLP处理ECV-304细胞后,与HL-60细胞的粘附率明显下降.这表明200 μg/ mL和500 μg/ mL剂量的GBLP对人脐静脉内皮细胞与HL-60细胞粘附有抑制作用.     

Requirement for ras proto-oncogene function during serum-stimulated growth of NIH 3T3 cells

Human tumours often contain DNA sequences not found in normal tissues which are able to transform cultured NIH 3T3 cells. In some tumours the gene responsible for this transformation belongs to the cellular ras gene family. A specific type of mutation is responsible for converting the cellular proto-oncogene into a ras oncogene capable of inducing transformation. In a study of the function of a cellular ras gene, its protein product (produced in a bacterial cell) was microinjected into NIH 3T3 cells; the recipient cells became morphologically transformed and were induced to initiate DNA synthesis in the absence of added serum, but only when cellular ras protein was injected at much higher concentrations than required with protein of the transforming ras gene. To further analyse the function of the cellular ras gene, we have now injected monoclonal antibodies against ras proteins into NIH 3T3 cells. We report here that NIH 3T3 cells induced to divide by adding serum to the culture medium are unable to enter the S phase of the cell cycle after microinjection of anti-ras antibody, showing that the protein product of the ras proto-oncogene is required for initiation of the S-phase in NIH 3T3 cells.     

盐和过氧化氢胁迫下交替氧化酶调节根生长和细胞死亡

用不同浓度的NaCl或外源过氧化氢(H_2O_2)溶液处理野生型(WT)拟南芥和交替氧化酶(alternative oxidase,AOX)基因反义抑制突变体(AS-12)拟南芥,发现随NaCl和过氧化氢浓度的增加,两种拟南芥幼根生长速率降低,且出现了明显的细胞死亡。在50和100 mM NaCl或在50 mM H_2O_2处理下,与WT植株相比,AS-12植株幼根的生长速率更低,且组织的细胞死亡程度也更加明显。150 mM NaCl或100及200 mM H_2O_2处理导致AS-12与WT幼根的生长停止和严重的细胞死亡,但两种植株幼根在生长速率和细胞死亡程度上无明显差别。以上实验结果说明交替氧化酶在一定水平的盐胁迫和氧化压力下能够影响幼根的生长和细胞死亡的发生。     

Raf-1 protein kinase is required for growth of induced NIH/3T3 cells

Many growth factors regulate the cytoplasmic Raf-1 protein kinase, consistent with its having a central role in transduction of growth signals. The kinase is ubiquitously expressed and can promote proliferation, presumably in a manner dependent on growth-factor receptors and membrane-associated oncogenes. We have now examined the dependence of serum- and TPA (12-O-tetradecanoylphorbol-13-acetate)-regulated NIH/3T3 cell growth on RAF-1 kinase to determine whether Raf-1 is essential for receptor signalling. We inhibited Raf-1 function by expressing c-raf-1 antisense RNA or kinase-defective c-raf-1 mutants. Antisense RNA for c-raf-1 interferes with proliferation of normal NIH/3T3 cells and reverts raf-transformed cells. In revertant cells, DNA replication induced by serum or TPA was eliminated or reduced proportionately to the reduction in Raf protein levels. Expression of a kinase-defective Raf-1 mutant (craf301) or a regulatory domain fragment (HCR) inhibited serum-induced NIH/3T3-cell proliferation and raf transformation even more efficiently. Inhibition by antisense RNA or craf301 blocked proliferation and transformation by Ki- and Ha-ras oncogenes. We conclude that raf functions as an essential signal transducer downstream of serum growth factor receptors, protein kinase C and ras.     

质子交换膜燃料电池(PEMFC)的原理及应用

简述了质子交换膜燃料电池的工作原理;质子交换膜燃料电池是最具商业前景的电动汽车用绿色能源,在航天领域、潜艇、电动车、电站等领域具有广泛的应用前途.     

Production of the haemopoietic growth factors GM-CSF and interleukin-3 by mast cells in response to IgE receptor-mediated activation

Mast cells have a central role in allergic diseases mediated by specific immunoglobulin E antibody responses to allergens. The binding of IgE to the high-affinity receptor for IgE (Fc epsilon R) on mast cells and basophils enables these cells to react specifically to allergens. Such contact leads to the activation of mast cells and the release of histamine and other pharmacological mediators, causing an immediate hypersensitivity and acute inflammatory reactions, accompanied by the development of allergic symptoms. Here we show that Fc epsilon R-mediated activation of murine mast cells results in the production of the haemopoietic growth factors granulocyte/macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3). IL-3 and GM-CSF, in addition to their role in bone marrow haemopoiesis, also influence inflammation as they have the capacity to recruit, prime and activate inflammatory cells such as neutrophils, macrophages and eosinophils. Secretion of these factors by mast cells in response to allergens may therefore have an important role in local tissue defense.     

Polymorphism in the alpha 3 domain of HLA-A molecules affects binding to CD8

Cytotoxic T lymphocytes (CTL) expressing the CD8 glycoprotein recognize peptide antigens presented by class I major histocompatibility complex (MHC) molecules. This correlation and the absence of CD8 polymorphism led to the hypothesis that CD8 binds to a conserved site of class I MHC molecules. Using a cell-cell binding assay we previously demonstrated specific interaction between human class I MHC (HLA-A,B,C) molecules and CD8. Subsequent analysis of the products of 17 HLA-A,B alleles revealed a natural polymorphism for CD8 binding in the human population. Two molecules, HLA-Aw68.1 and HLA-Aw68.2, which do not bind CD8, have a valine residue at position 245 whereas all other HLA-A,B,C molecules have alanine. Site-directed mutagenesis shows that this single substitution in the alpha 3 domain is responsible for the CD8 binding phenotype and also affects recognition by alloreactive and influenza-specific CTL. Our results indicate that CD8 binds to the alpha 3 domain of class I MHC molecules.     

蜂窝状无线网集成语音数据业务的资源分配模型

本文提出了一个全新的呼叫接入方案,以有效使用蜂窝技术和无线网络。这个方案为两种存取网络采取了不同的资源分配策略,根据不同的呼叫层级服务质量(QoS)需求,限制新的水平或垂直的用于信号切换的语音数据的呼叫到达。数值结果显示,与没有集成的网络模型相比,本方案更加有效,对语音数据用户而言满足所有达到最大值的QoS需求。     

Upregulation of annexin A5 affects the biological behaviors of lung squamous carcinoma cells in vitro

Annexin A5 is a Ca2＋-dependent phospholipid- binding protein and protein kinase C inhibitory protein. It has a potential role in cellular signal transduction, inflam- mation, growth and differentiation. In this study, we evaluated the expression of this protein in lung tumor tis- sues and subsequently established a NCI-H520 cell line that stably expresses the wild-type ANXA5 gene to deter- mine the effects of annexin A5 upregulation on the cell morphology, proliferation and metastasis potential in vitro. The effects of annexin A5 on NCI-H520 cells were tested by crystal violet staining, CCK-8 assay, scratch wound assay, and Transwell assay. The expressions of Akt, PCNA, vimentin, and E-cadherin were examined by Western blot assay. In this study, we demonstrated that annexin A5 is expressed at lower levels in tumor tissues compared with normal tissues. Additionally, the upregu- lation of this protein may inhibit the proliferation, migra- tion, and invasion abilities of NCI-H520 cells in vitro. Thetransfected cells were arrested in the G1/S phase of the cell cycle, and the expression levels of Akt, PCNA and Vimentin were downregulated, while E-cadherin was upregulated.