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1.
黑曲霉(Aspergillusniger728)的粗酶液,经硫酸铵沉淀,SephadexG-25柱凝胶过滤脱盐,DEAE-Toyopearl离子交换柱层析和SephacryS-100凝胶层析纯化,经PAGE鉴定为一条带SDS凝胶电泳测定分子量为70000.金属离子Fe3+对该酶有一定的抑制作用,该酶的等电点为3.7,最适pH为4,最适温度为60℃.E2801%为15.72,Km值为0.112×10-3m.  相似文献   

2.
花椰菜凝集素由花蕊组织经生理盐水抽提、硫酸铵沉淀和SephadexG-100、Sepharose-4B凝胶层析纯化获得.PAGE鉴定和高碘酸-Schiff试剂反应均显单一条带,说明该凝集素为纯化的糖蛋白.经SephadexG-200柱过滤分析,其相对分子质量约为94000.SDS-PAGE分析表明该凝集素含有4个亚基,其相对分子质量分别约为28000、25000、22000和20000.花椰菜凝集素经50℃处理5分钟仍保持高的凝集活性,该凝集素对酸处理较碱处理稳定.  相似文献   

3.
玉米螟谷胱甘肽转硫酶的纯化及性质研究   总被引:1,自引:0,他引:1  
以亚洲玉米螟为材料,以经Sephadex-G75、DEAE-SepharoseFast Flow、还原型谷胱甘肽(GSH)为配基的亲和层析,分离纯化得到玉米螟谷膛甘肽转流酶(GST),以2,4-二硝基氯苯(CDNB)为底物测得其比活为1070U/mg蛋白,收率为22.9%,提纯1138倍,经SDS-PAGE和PAGE鉴定,得到的GST为一条带,亚基的分子量为25kD,经凝胶过滤测得其全酶分子量为5  相似文献   

4.
将小麦麦胚和麸皮的浸取液,经加热和利用pH沉淀,获得疏基蛋白酶抑制剂粗品;再经DEAE-Sepharose,Sephadex G-100分子筛层析,在SDS-PAGE和HPLC柱上得到均为单一蛋白带的小麦CPI纯品。其纯化度为原料浸液的42倍。由SDS-PAGE测得CPI分子为单一肽链  相似文献   

5.
用DEAE-Sephadex-A-5-分离竹叶青粗毒,再经CM-Sepharose-CL-6B、QAE-Sephadex-A-50和SephadexG-100纯化,得到一个5-核苷酸酶,对血小板聚织要具有强抑制作用,在SDS-聚丙烯酰谘电泳图谱上为一单一蛋白带,分子量为82500,是一个糖蛋白,含6.7%中性糖和0.7%唾液酸。  相似文献   

6.
应用葡聚糖凝胶柱层纯化了水牛梭形住内孢子虫缓殖子抗原,并应用SDS-PAGE分析了缓殖子可溶性粗抗原及纯化抗原的蛋白组分,结果表明:缓殖子可溶性粗抗原含有14种蛋白成分,分子量范围为45.9-71.2KD,经过SephadexG-100柱层析纯化扣的抗原在SDS-PAGE图谱上显示5条蛋白带,分子量分别为58KD,61KD,64KD,66KD,70KD。  相似文献   

7.
猪凝血酶的分离纯化与鉴定   总被引:6,自引:0,他引:6  
本文报导以猪血为材料,制备凝血酶,血浆在低离子强度下经等电沉淀得优球蛋白,从该沉淀中采用稀盐溶液提取,氢氧化镁吸附和二氧化碳解吸等步骤获得凝血酶原粗品。后者经脑提取液激活,再经乙醇分级、DEAE-纤维素与磷酸纤维素离子交换层析和SephadexG-100凝胶过滤,得凝血酶制品,经SDS-PAGE分析,制品呈单一条带,分子量约为39kD,比活达1610NIHU/mg蛋白,以活化的粗品活性为100%,活性回收率为48%,纯化倍数约为10。  相似文献   

8.
孕发尿液蛋白丙酮粉粗提液经Bio-gelP-10凝胶层析获得五个组分,经免疫点印渍分析后,证明其中组分Ⅲ的活性最高,该组分再经SephadexG-15凝胶层析,又得到A、B、C和D四个组,hEGF活性主要分布在组分A中。本文建立了免疫点印渍方法并应用于hEFG的活性检测。  相似文献   

9.
牛奶黄嘌呤氧化酶的纯化性质研究   总被引:1,自引:0,他引:1  
本文建立从牛奶中分离纯化黄嘌呤氧化的酶的工艺,该工艺采用正丁醇-硫酸铵分级提取、磷酸钙凝胶吸附、SephadexG-150层析纯化获得纯品。从磷酸钙凝胶上解吸到的XOD粗酶化活性达5.096I.U/mg-pr,提纯340倍,产率达74.3%。每单位粗酶的吸附剂用量以6mg为最佳。纯酶的最大紫外吸收在285nm处,酶活性测定的最适pH在8.60~10.15之间,最适温度在25℃~35℃间。1mol/  相似文献   

10.
黑曲霉单宁酶的纯化及部分性质研究   总被引:7,自引:0,他引:7  
黑曲霉在五倍子单宁的诱导下产生单宁酶。通过(NH4)2SO4分形沉淀,DEAE-Sepharose离子交换层析,Sephadex G-150凝胶过滤,从黑曲霉的发酵液和菌丝中纯化得到凝胶电泳均一的单宁酶。酶作用的最适温度为30℃,最适pH值为5.5,在温度10-30℃和pH4-6之间保持稳定,金属离子对单宁酶没有的激活作用,Fe^2+和Mn^2+则对酶有抑制作用。  相似文献   

11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.  相似文献   

12.
何延凌 《科技信息》2008,(4):258-258
Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women's language from these perspectives: pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women's language, more attention should be paid to language use in social context. What's more, the linguistic phenomena in a speaking community can be understood more thoroughly.  相似文献   

13.
There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.  相似文献   

14.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。  相似文献   

15.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

16.
王慧 《科技信息》2008,(10):240-240
Wuthering Heights, Emily Bronte's only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.  相似文献   

17.
The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.  相似文献   

18.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.  相似文献   

19.
In the 19th century the society was controlled by men, and women were just appendants of them, they had not any rights and freedom. But Jane was an exception, she showed some characteristics of early feminist. Jane showed her characteristics of feminism in three aspects: rebellion, equality, and independence. These characteristics were helpful to her success, and feminism is the only way out for women of that time.  相似文献   

20.
1 Rise of studies on climate change's effects on biodiversity
Until the 1980s, climate change and biodiversity were studied as two independent disciplines for more than a century. In 1992, the Ecological Society of America's annual report named climate change, biodiversity, and the sustainable ecological system as the three major global environmental issues of the twenty-first century [1].  相似文献   

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