平消胶囊联合紫杉醇对小鼠黑色素瘤生长与转移的抑制作用

本研究通过建立黑色素瘤细胞(B16)的皮下异体移植瘤模型和肺转移模型,探索平消胶囊对临床广谱抗肿瘤药紫杉醇抑制黑色素瘤细胞生长和转移的影响,旨在为临床用药提供参考.实验结果显示,平消胶囊单用可有效抑制皮下移植瘤的生长,并可抑制肿瘤的肺转移,与紫杉醇联用可提高后者抑制移植瘤生长和转移的活性,作用机制可能与降低动物血清中IL-10的水平有关.平消胶囊与紫杉醇组成的给药方案可增强紫杉醇的抗肿瘤效果.     

AS-mCLB1重组质粒联合多西紫杉醇抗Lewis肺癌细胞增殖活性的研究

pAS-mCLB1转染Lewis肺癌(LL/2)细胞72 h后,再加用多西紫杉醇(40 nmol/L)处理细胞,MTT法测定药物对LL/2细胞的体外杀伤作用.另将LL/2细胞接种C57BL/6小鼠,成瘤后分别用pAS-mCLB1、多西紫杉醇和pAS-mCLB1+多西紫杉醇处理动物,3天1次,共4次,观察肿瘤体内增殖情况,流式细胞仪检测动物肿瘤组织的细胞凋亡.结果显示：pAS-mCLB1联合多西紫杉醇可明显降低LL/2细胞体内、外增殖活性,同时促使肿瘤组织中细胞凋亡增加,两种方法具有协同作用.pAS-mCLB1显著增强多西紫杉醇抗肿瘤效应,此增强作用可能与pAS-mCLB1诱导肿瘤细胞凋亡,提高了多西紫杉醇的细胞毒作用有关.     

不同溶剂浸取红豆杉细胞中的紫杉醇的研究

用７种单一溶剂浸取红豆杉细胞中的紫杉醇,研究表明,甲醇有最好的浸取效果,紫杉醇含量占细胞干重的０．０３３１５％,在２３种混合溶剂中,ＣＨ３ＣＯＯＣ３Ｈ５／Ｃ２Ｈ５ＯＨ,ＣＨ３ＣＯＣＨ３／ＣＨ３ＯＨ,ＣＨ３ＣＯＣＨ３／Ｃ２Ｈ５ＯＨ,ＣＨ３ＯＨ／ＣＨＣｌ３浸取效果最好,其紫杉醇含量占细胞干重分别为０．０５１６６％,０．０４９２３％,０．０４２７６％,０．０３９８１％。     

Chitosan对红豆杉PAL及Taxol合成的影响

研究了Ｃｈｉｔｏｓａｎ（聚氨基葡糖）对红豆杉悬浮培养细胞ＰＡＬ（苯丙氨酸解氨酶）活性及Ｔａｘｏｌ（紫杉醇）生物合成的影响．种胚来源的红豆杉细胞培养在改良ＭＳ液体培养基中,于培养的第１８ｄ添加不同浓度的Ｃｈｉｔｏｓａｎ．Ｃｈｉｔｏｓａｎ浓度为１００～１０００ｍｇ·Ｌ－１时对红豆杉细胞ＰＡＬ活性有明显的诱导作用,且诱导作用随浓度的增加而增强,在诱导作用下ＰＡＬ活性在１６ｈ达到峰值;Ｃｈｉｔｏｓａｎ浓度在１００ｍｇ·Ｌ－１时对红豆杉细胞的生长基本无抑制作用,增产效果最显著（约为对照组的１０倍）,Ｃｈｉｔｏｓａｎ可作为Ｔａｘｏｌ合成的诱导子．     

细胞悬浮培养制备紫杉醇的研究进展

紫杉醇具有很强的抗癌活性,药用价值很高.综述了生物合成、微生物发酵和细胞悬浮培养制备紫杉醇的研究进展,重点阐述细胞培养过程中外植体的选取、培养基的组成和诱导因子等对紫杉醇产率的影响.     

超临界流体重结晶吸附法分离富集紫杉醇粗品研究

比较用超临界流体ＣＯ２萃取,重结晶及重结晶－吸附法对紫杉醇粗品浸膏（含Ｔａｘｏｌ约１．０％）快速分离富集进行了研究,结果表明用重结晶－吸附法１次可快速使粗品紫杉醇浓缩富集１０倍左右,分析结果用ＨＰＬＣ定量测定     

恩度联合紫杉醇对三阴型乳腺癌移植瘤的影响

目的:通过观察人三阴型乳腺癌在裸鼠模型中血管正常化后对紫杉醇的反应,探讨其疗效是否优于紫杉醇.方法:将人三阴型乳腺癌MDA-MB-231细胞株种植于BALB/c-nu雌裸鼠右下腹部皮下,随机将符合入组标准的28只移植瘤荷鼠分成4组,每组7只,A组,每天每次腹腔内注射生理盐水(质量分数10 mg/kg),连续17 d;B组,每天每次腹腔注射恩度(质量分数10 mg/kg),连续17 d;C组,第6、12天腹腔注射紫杉醇(质量分数10 mg/kg),余同A组;D组,第6、12天腹腔注射紫杉醇(质量分数10 mg/kg),余同B组.测量移植瘤的长径和短径,进行病理学检测.结果:D组移植瘤体积小于A组、B组(P0.05),C组移植瘤体积小于A组及B组(P0.05),D组和C组移植瘤体积无差别(P=0.436).B组、C组、D组的抑瘤率分别是14.61%、39.08%、54.79%.解剖各组荷鼠未见远处转移病灶.病理检查微血管密度,D组C组=B组A组.Ki67,D组与C组A组与B组,D组与C组无差别(P=0.649).结论:使用恩度后在时间窗内联合紫杉醇有明显抑瘤作用,但疗效未优于紫杉醇.     

IDO抑制剂LPM3480226联合不同化疗药物抗小鼠结肠癌CT26

研究吲哚胺2,3-双加氧酶(IDO)抑制剂LPM3480226与不同化疗药物联合使用对小鼠结肠癌CT26移植瘤生长的抑制作用,为IDO抑制剂的应用提供临床前药效学依据.采用CT26皮下移植瘤小鼠模型,通过观察荷瘤小鼠体重、肿瘤体积、肿瘤重量等指标,考察LPM3480226分别与紫杉醇、伊立替康、力扑素(紫杉醇脂质体注射...     

利用高压液相色谱法测定红豆杉组织培养物中紫杉醇的含量

报道了以高压液相色谱法测定红豆杉组织培养物中的紫杉醇含量,并与原植物的ＨＰＬＣ图谱进行了对比。认为组培法可以富集原植物中紫杉醇和三尖杉酯碱,使其含量得以提高。以三氯甲烷∶甲醇＝６∶４的提取方法及流动相为甲醇－乙晴－水（２５∶２４∶４１）的条件,可准确、简便地测定组织培养物中紫杉醇含量。几组红豆杉培养物的ＨＰＬＣ图谱上出现了新的次生代谢产物     

东北红豆杉中紫杉醇的含量分析

以抗癌活性成分紫杉醇作为标准物,利用高压液相色谱法分析了东北红豆杉树叶和树干的水提取物中抗癌活性成分紫杉醇的含量,确定最佳色谱条件为:色谱柱:C18,Φ4.6×250 mm;流动相:甲醇︰水︰乙腈=45︰35︰20;检测波长:225 nm;柱温:室温;流速:1.0 mL/min。结果表明:东北红豆杉树干中紫杉醇含量高于树叶。     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.