支持向量机程序SVMProt预测SArs病毒蛋白质的功能

对SARS冠状病毒蛋白质功能的有效识剐将有利于促进SARS传染病治疗药物的开发。应用基于支持向量机原理的SVMProt程序识别SARS冠状病毒蛋白质的功能,通过对SARS冠状病毒中2个已知功能的蛋白质功能的成功预测,说明SVMProt能够有效地应用于SARS冠状病毒蛋白质及其他种类蛋白质的功能预测。对SARS冠状病毒中至今仍未知其功能的蛋白质ORFl3的功能进行了预测,结果显示ORFl3是一种可能与DNA结合的核蛋白并兼有病毒体内结构蛋白的功能。     

基于生物信息学与分子结构的SARS冠状病毒主蛋白酶(SARS CoV Mpro)多肽类抑制剂研究

用冠状病毒基因解析软件系统ZCURVE_CoY2．0，从SARS冠状病毒（TOR2，NC_004718）的RNA基因序列出发，搜索出含有SARS冠状病毒主蛋白酶（SARS CoV M^pro）真实剪切位点的11个八肽，运用分子叠合和分子对接的方法，对11个八肽进行了分析，证明这11个八肽有相似的活性，而且11个八肽中，op4的活性最高．分析认为，op4有望成为抗SARS药物的理想前体．     

DNA分析技术鉴别乌头初探

该文尝试建立一种利用DNA检验技术鉴别乌头的方法。通过提取50份乌头类及25份其他植物样本的DNA,用荧光特异性引物对乌头ITS2序列进行扩增,产物经3130XL测序仪进行检测分析,并进行测序入库比对。结果发现,试验中所有乌头类植物得到168bp的扩增产物,而其他对照植物均无扩增产物。由此表明,DNA分析技术对于鉴别乌头类与其他植物有一定的参考借鉴价值。     

线粒体连续超薄切片电镜图像的三维可视化

研究建立对连续超薄切片电镜图像的三维可视化方法。对正常及用SARS冠状病毒感染非洲绿猴肾细胞（VeroE6）样品进行连续超薄切片，通过透射电镜得到线粒体断层图像，用表面显示技术对线粒体进行三维重构显示。正常和SARS冠状病毒感染的VetoE6细胞中的线粒体在结构上有明显区别，揭示出SARS冠状病毒感染VeroE6细胞线粒体的病理改变。以此建立起电镜连续超薄切片线粒体图像三维可视化方法。     

葡萄病毒B优化检测体系的建立

为了解决种苗检疫、抗病性早期鉴定等基本问题,为葡萄无毒化栽培的提供基本保障。本实验从感染葡萄病毒B的葡萄皮层中提取总RNA,以其为模板进行一步RT-PCR扩增,并利用此扩增产物为模板进行二次扩增,二者均能获得与预期片段大小一致长约460bp和243bp的扩增产物,在此基础上,建立了萄病毒B的一步RT-PCR、巢式PCR以及斑点杂交优化检测体系。利用本实验所建立的优化检测体系对葡萄样本进行检测,表现出了良好的特异性与稳定性。     

微量植物组织汁液直接RT-LAMP可视化检测马铃薯卷叶病毒方法的构建

为建立快速的马铃薯卷叶病毒检测方法以应用于脱病毒后大规模样本的检测,构建利用微量植物组织汁液直接逆转录环介导恒温扩增(RT-LAMP)可视化检测马铃薯卷叶病毒的方法,即以无菌大头针的针尖刺马铃薯植株茎尖时所粘附的汁液直接作为RNA模板,采用Bacillus stearothermophilus(Bst) DNA聚合酶进行RT-LAMP扩增,扩增产物通过肉眼观察有无白色沉淀来诊断马铃薯卷叶病毒.该方法快速、操作简单、成本低,在大规模组织培养无病毒种薯生产中具有很大的应用空间.     

河北省新乐市羊梨形虫分子检测

为检测河北省新乐市羊梨形虫的感染情况,采集河北省新乐市羊血液样本31份,并提取基因组DNA;根据梨形虫18S rRNA基因序列设计5对特异性引物,以羊血液基因组DNA为模板,对梨形虫进行巢式PCR扩增。结果表明,在31份DNA样本中检测出4份吕氏泰勒虫(12.9%),而尤氏泰勒虫、中华泰勒虫和羊巴贝斯虫均未检测到阳性样本。     

DNA短片段复合扩增系统鉴别大麻研究

通过提取大麻及其他对照植物样本的DNA,用荧光标记的特异性引物进行扩增,产物经3500XL测序仪进行检测分析,同时以理化LC-MS/MS分析方法和大麻性别检验法对样本进行检测,与DNA检测结果进行相互印证。结果发现,毒品大麻在93bp与157bp处检测到2条特异性扩增条带,而纤维大麻仅在93bp处检测出1条特异性条带,检测结果与大麻性别检验法得出一致结论,与理化LC-MS/MS分析方法得出的结果有差异。由此可见,该文建立的短片段复合扩增系统对于毒品大麻与纤维大麻的鉴别有一定参考价值。     

SARS冠状病毒核蛋白的质谱鉴定

利用质谱技术,对纯化的SARS冠状病毒的核蛋白进行初步鉴定与分析.所获得的质谱分析数据中,分子量为2881.19Da的酶切肽段的氨基酸序列与其它冠状病毒的同源性比较分析表明,SARS冠状病毒和其它冠状病毒均含有一个高度保守的基序“FYYLGTGP”.     

马铃薯卷叶病毒基因组保守序列片段的RT—PCR扩增

从感染马铃薯卷叶病毒的植株中提取马铃薯卷叶病毒总RNA,针对马铃薯卷叶病毒基因组,其中的2个保守序列分别设计并合成了1对寡聚核苷酸引物,用反转录-聚合酶链式反应方法从提取的病毒RNA材料中扩增出符合设计大小的240bp、400bp的特异性产物,对照的健康植株中未扩增出相应产物。     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

Perspherical Approaches for Electrons in Atoms and Charged Excitons in Quantum Wells

Instead of following Fock’s expansion,we solve the Schrodinger equation for some quantum mechanical manybody systems such as electrons in atoms and charged excitons in quantum wells in a similar way in hyperspherical coordinates by expanding the wave functions into orthonormal complete basis sets of the hyperspherical hannonics(HHs)of hyperangles and generalized Laguerre polynomials(GLPs)of the hyperradius.This leads the equation to     

Advances in broadband wireless communications under high-mobility scenarios

Future mobile communication systems aim at providing very high data transmission rates, even in high-mobility scenarios such as high-speed wheel-track trains, maglev trains, highway vehicles, airplanes, guided missiles or spacecraft. A particularly important commercial application is the strong and increasing worldwide demand for high- speed broadband wireless communications （up to 574.8 km/ h test speeds or 380 km/h commercial speeds） in railways, providing data, voice and video services for applications such as onboard entertainment services to passengers, train control, train dispatch, train sensor status handling and sur- veillance. In such high-mobility scenarios, there are a number of communication challenges, including fast hand- over, location updating, high-speed channel modeling, estimation and equalization, anti-Doppler spreading tech- niques, fast power control, and dedicated network architec- ture. Because signal transmission in very high-speed scenarios will inevitably experience serious deterioration, it is imperative to develop key broadband mobile communi- cation techniques for such high-speed vehicles.     

Wuhan University Journal of Natural Sciences TOTAL CONTENTS

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Scale-based spatial data model for GIS

Being the primary media of geographical information and the elementary objects manipulated, almost all of maps adopt the layer-based model to represent geographic information in the existent GIS. However, it is difficult to extend the map represented in layer-based model. Furthermore, in Web-Based GIS, It is slow to transmit the spatial data for map viewing. In this paper, for solving the questions above, we have proposed a new method for representing the spatial data. That is scale-based model. In this model we represent maps in three levels: scale-view, block, and spatial object, and organize the maps in a set of map layers, named Scale-View, which associates some given scales.Lastly, a prototype Web-Based GIS using the proposed spatial data representation is described briefly.     

英文诗歌中的喻类修辞

为了有助于提高英文爱好者的个人情操、文化素养和学习兴趣,以及专业人员对英语学习和研究进行多途径的探索,本文通过一些精选诗歌的引证和分析,着重论述了喻类修辞法在英文诗歌中的运用其及效果。     

Man Is Unable to Change the Miserable Life——Analysis of the Arrangement of Four Characters in The Glass Menagerie

Tennessee Williams is considered as one of the most important American playwrights since World War II.The Glass Menagerie is his first successful drama,which describes a tragic situation of family and means to say that Man is unable to change the miserable life,no matter whatever means he try,This essay focuses on the analysis of the arrangement of the four main characters:Laura.Amanda,Jim and Tom to reveal the theme.Laura is fragile.Amanda is brave.Jim is vital.Tom is sensible.And all of them develop and try the different means to struggle against life,but fail tragically.With the evidence,the paper comes to conclusion naturally that Man is unable to change the miserable life,and he dooms to fail.     

Characterization of root-nodulating bacteria on Retama raetam in arid Tunisian soils

The aim of this study is to investigate the diversity of Retama raetam root-nodule bacteria isolated from arid regions of Tunisia. Twelve isolates, chosen as representative for different 16S rRNA gene patterns, were characterized by 16S rRNA gene sequencing and phenotypic analysis. Isolates were assigned to Sinorhizobium, Rhizobium and Agrobacterium. Symbiotic properties of Sinorhizobium and Rhizobium isolates showed a large diversity in their capacity to infect their host plant and fix atmospheric nitrogen. Strain RK 22 identified as Rhizobium was the most effective isolate.     

An Affordable Accurate Evaluation of the Binding Conformations Predicted by Molecular Docking Studies in Bio-System

正Recently,docking has been widely used to predict the binding-modes of protein-inhibitors,when the crystal complexes structure was absent.Most docking algorithms are able to generate a large number of probable conformations,it,however,is difficult to effectively evaluate these docking poses and identify the most reasonable bindingmode.In the present study,on the basis of the crystallographic data of human 3-hydroxy-3-methylglutaryl coenzyme     

WIMAX与WI-FI技术分析及联合组网

介绍了WiMAX与Wi-Fi两种无线宽带接入技术,并对两者之间的关系及相互之间的影响做了对比及分析,并对WiMAX的关键技术进行了详细说明,最后对两者的联合组网方式做了简单的探讨。