微束激对单细胞诱变机制研究

本文报道用微束激光对家蚕进行外源染色质的转移获得成功，观察到转移的染色质基因在受体中的当代形态性状表达，并论述短脉冲微束激光照射家蚕 精卵大细胞转移外源基因的同时，引起变异的主要机制。     

微束激光对单细胞诱变机制研究

本文报道用微束激光对家蚕进行外源染色质的转移获得成功,观察到转移的染色质基因在受体中的当代形态性状表达.并论述短脉冲微束激光照射家蚕受精卵大细胞转移外源基因的同时,引起变异的主要机制.     

毫秒激光加工小孔与重铸层的后处理工艺

利用钇铝石榴石晶体(Nd:YAG)毫秒激光器以正交实验法在1Cr13不锈钢材料上进行加工实验,得出在现有条件下具有最小重铸层微小孔的脉冲激光优化参数(如脉宽、峰值功率、频率、离焦量).采用一种化学酸溶液对按此优化参数加工出来的微小孔进行腐蚀,发现腐蚀后孔壁上激光加工形成的重铸层已被完全去除,表明由脉冲激光优化参数加工出的微小孔能够很好地配合化学酸溶液的腐蚀.该复合加工方法具有成本低、效率高、操作简单的特点,不仅解决了高能束热源在金属上加工微小孔时常有重铸层及微裂纹缺陷的问题,而且得到了孔壁光滑、轮廓清晰的高质量微小孔.     

激光微光束仪的设计和研制

红宝石脉冲激光微光束仪用于生物活细胞的微照射和解剖.本文详细分析和讨论了仪器总体设计的特点.论述激光微光束的理论计算.为了获得小的聚焦光斑直径和高的聚焦效率,较佳的折衷方案是选择低倍的显微镜目镜和高倍大数值孔径的显微镜物镜.而且,为了获得小的聚焦光斑尺寸,物镜必须严格校正球差,并且激光器应是基模运转的.文中还详细讨论了减少激光束通过显微镜光能量损失的方法,以提高聚焦系统的效率.     

纳米尺度激光紧聚焦光穿孔技术

论述了一种新型的细胞转导方法——纳米尺度激光紧聚焦光穿孔技术.按照光穿孔可能的物理机制,分4类讨论了现在主流的光穿孔技术:连续激光光穿孔,其中最前沿的技术是紫外二极管激光光穿孔;纳秒、皮秒激光脉冲和经放大的千赫兹飞秒激光脉冲光穿孔,其中应用范围最广的是Nd:YAG激光器;飞秒激光振荡器发出的兆赫兹脉冲序列光穿孔,该技术在多尺度、跨学科及在体研究中扮演重要角色;最新出现的利用双脉冲微射流进行光穿孔,这是光穿孔技术中仅有的一种将外源物主动引入细胞内的方法.可以预见:当激光紧聚焦技术结合了双光子荧光显微镜、微芯片实验室、膜片钳技术等各种单细胞分析技术后,必将在细胞间通信、干细胞谱系提交、癌症转移、激光基因疗法、胚胎发育学以及神经功能再生等学科领域中产生重大影响.     

一种YAG激光眼科治疗仪的光学系统

介绍了一种分体测立式ＹＡＧ激光眼科治疗仪的光学系统。治疗头与裂隙灯显微镜光学系统相互独立，它具有二级扩束，Ｈｅ－Ｎｅ光对ＹＡＧ光共轴指示，ＹＡＧ激光焦斑直径ｄＹＡＧ＝（３０±５０）μｍ，光强度满足进行眼科手术的要求。     

利用脉冲激光技术研究哺乳动物碳氧血红蛋白光解量子产率

本文利用脉冲光解法测量了哺乳动物碳氧血红蛋白的光解反应的量子产率.脉冲光解法是利用一束波长为532 nm、脉冲宽度为8 ns、重复频率为10 Hz的脉冲激光照射碳氧血红蛋白溶液,使其发生光解反应.考虑到碳氧血红蛋白溶液和其光解产物脱氧血红蛋白的光吸收系数不同,可利用另一束波长为432 nm的连续激光检测溶液在光解前、后...     

单光刀与单光镊激光微束系统

提出了构建一种用于细胞非接触操作的激光微束系统。该系统由 Nd:YAG激光束经声压、热膨胀、汽化等综合效应实现的光刀和 He- Ne激光束经光学动力学效应实现的光镊组成。将两激光束耦合到显微镜中 ,实现了生物细胞的捕获、移动、翻转、打孔等一系列操作。在此基础上 ,分析了形成光镊所必需产生梯度力场的条件和形成光刀对能量的要求 ,进行了系统的总体设计、关键部件设计和选择 ,构建了一套激光微束操作实验系统 ,得到了预期的试验结果。在该系统上成功地实现了非接触细胞操作 ,并对染色体进行了切割。     

荧光检验法判定激光打印文件朱墨时序

目的在文件检验鉴定实践中,如何判定朱墨时序是常常碰到的课题,本文研究如何利用显微镜的荧光检验功能对激光打印文字与印文交叉部位色料的荧光特性变化规律来判定激光打印文件朱墨时序。方法使用ZEISS Discovery.V20荧光显微镜,选择合适的激发光源对实验样本的交叉部位和非交叉部位进行照射,观察、分析、比较和归纳两种时序下的荧光现象。结果先朱后墨,交叉部位笔画易产生缺损或不完整现象,在缺损处可见黄色强荧光,未缺损处墨迹边缘虚化,在笔画边缘或笔画上出现点状或线状黄色弱荧光;先墨后朱,交叉部位笔画完整,墨迹颜色变浅,并在笔画上可见片状或斑点状黄色强荧光。结论利用荧光检验法可以准确判定激光打印文件朱墨时序,为判定朱墨时序提供一种新途径。     

激光旋切法加工高质量微小孔工艺与理论研究

针对激光微小孔加工中微小孔几何形貌和孔壁重铸层这2个影响微小孔加工质量的关键因素,利用大功率Nd:YAG毫秒脉冲激光器,分别在304不锈钢和DZ445定向结晶镍基合金上进行孔加工实验,主要研究了激光旋切法加工微小孔的工艺特点,对旋切法所涉及的3个关键参数(旋切路径、旋切速度、旋切圈数)对孔质量的影响规律进行了深入探讨。结果显示:边缘起点的旋切路径易导致孔缘出现缺口,而圆心起点的旋切路径可以避免这种现象;旋切速度和旋切圈数对孔壁重铸层厚度影响显著,重铸层厚度随着旋切速度的降低和旋切圈数的增加而减小,其机理在于,孔壁重铸层在旋切过程中会因激光的重复照射再次发生熔化,并在气化压力与辅助气压等驱动力的共同作用下克服黏滞力发生质量迁移,进而从孔出口排出,在其他工艺条件不变的情况下,重铸层的厚度与质量迁移的持续时间成反比。     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.