蛹虫草菌丝体液体培养条件研究

研究蛹虫草液体发酵条件,以干菌体得率为指标,通过单因素试验确定适宜的碳源、氮源、培养温度和pH值。结果表明,最适液体培养基为:葡萄糖20 g/L,蛋白胨5.0 g/L,最适pH=6.0,最适培养温度24℃。     

蛹虫草液体的深层发酵

以菌丝体质量浓度(干重)和发酵液中甘露醇浓度为指标,研究温度、初始pH值、培养时间等因素对蛹虫草液体培养的影响,得到蛹虫草摇瓶液体培养的最佳温度为25.0℃,初始pH值为6.10,培养时间为5 d;蛹虫草液体发酵最适培养基的组成为:20 g/L蔗糖+5 g/L蛋白胨+1 g/L MgSO4.7H2O。在此基础上进行小型分批式发酵罐实验,研究有利于蛹虫草生长及活性物质生成的pH控制策略。研究结果表明,pH分段控制的液体深层发酵对蛹虫草生长最为有利,菌丝体质量浓度为17.31 g/L,甘露醇质量浓度达43.47 g/L。     

蛹虫草纤溶酶液体发酵条件的优化

在发现蛹虫草具有纤溶酶活性的基础上,对蛹虫草产纤溶酶的液体发酵条件进行了优化,以期获得最佳的产酶条件。研究结果表明,蛹虫草纤溶酶的最优培养基组成是：以20 g/L可溶性淀粉为碳源,10 g/L酵母粉为氮源,Mg2＋浓度为7 mmol/L;最适发酵初始pH值为6;最适发酵时间为4 d。     

蛹虫草菌丝液体发酵产纤溶酶的实验研究

通过对蛹虫草(Cordyceps militaris)菌丝液体发酵产纤溶酶的实验研究,获得了蛹虫草纤溶酶液体发酵的适宜参数.结果表明,蛹虫草菌丝液体发酵产纤溶酶的适宜碳源为玉米粉和蔗糖,豆饼粉和较高浓度的玉米浆(8%~12%)作为氮源是适宜的,培养基中适量添加Mg SO4(0.05%)、KH2PO4(0.1%)和Ca Cl2(0.05%)、Mn SO4(0.05%)等无机盐有利于蛹虫草纤溶酶产生;发酵p H值以4.5~5.5为宜,温度以22~24℃为宜,培养时间以6~7 d为宜.     

美味牛肝菌深层发酵的研究

采用摇瓶培养法对美味牛肝菌(BoletusedulisBull.exFr.)适宜的发酵培养基、发酵条件进行了研究.通过L9(34)正交实验确定了美味牛肝菌发酵的最适培养基为:葡萄糖20.0g,酵母浸出膏7.0g,KH2PO42.0g,MgSO4·7H2O1.0g,VB10.01mg;适宜的发酵条件为:培养基初始pH5.2～5.8、振荡速度180r/min、培养温度27℃、100mL三角瓶装液量60mL、发酵周期6d.     

蛹虫草高产菌丝体和虫草菌素的深层培养工艺优化(英文)

蛹虫草Cordyceps militaris是当前用于功能食品和药物开发上较受国内外关注的真菌之一。本研究通过1次一因素法和正交实验设计优化了蛹虫草产虫草菌素和菌丝体的深层培养工艺。最适合菌丝生长的pH值和温度为6.0和20℃,而最适合虫草素积累的pH值和温度为5.0和26℃。蔗糖、蛋白胨、MgSO4、K2HPO4和NAA分别是最适合虫草素积累的碳源、氮源、无机盐和生长因子;培养基的成分对蛹虫草生物量的影响次序为:蔗糖>蛋白胨>MgSO4·7H2O>K2HPO4>NAA;培养基的成分对蛹虫草产虫草素的影响次序为:蛋白胨>K2HPO4>NAA>MgSO4·7H2O>蔗糖。最适合蛹虫草菌丝生长的培养基配比为4%蔗糖,2.5%蛋白胨,0.05%MgSO4·7H2O,0.15%K2HPO4,2.0mg/l NAA;最适合蛹虫草深层发酵产虫草素的培养基配比为4%蔗糖,1.5%蛋白胨,0.05%MgSO4·7H2O,0.05%K2HPO4,4.0mg/l NAA的培养基;在最佳蛹虫草深层发酵产虫草素的工艺条件下,CM8菌株胞外虫草菌素产量达到961.21 mg/l,胞内虫草菌素的含量达到12.87mg/g;菌丝体的产量为22.97 g/l,本研究得出的结论对深层培养提高蛹虫草虫草菌素和菌丝体的产量有借鉴意义。     

蛹虫草液体发酵培养基的筛选

本文为了获得在蛹虫草液体发酵培养条件下,菌丝体干重得率和干菌丝体多糖含量综合较高的培养基.通过精选15个培养基配方进行了液体摇瓶发酵试验.结果表明:筛选出的4号作为最适培养基,其菌丝体干重得率为23.32g/L,干菌丝体多糖含量为4.27%.     

蛹虫草液体培养联产胞外多糖与菌丝虫草素的实验研究

通过对蛹虫草(Cordyceps militaris)液体发酵产胞外多糖和菌丝虫草素的初步实验研究,获得了蛹虫草液体发酵联产胞外多糖和菌丝虫草素的适宜参数.研究结果表明,蛹虫草液体发酵联产胞外多糖和菌丝虫草素的较适宜的氮源是豆粕粉和6%~8%玉米浆粉;较适宜的碳源是玉米面;维生素B1添加量以10 mg/100 m L、微量元素锰添加量以0.01%Mn SO4为宜、培养基初始p H值以5.5为宜;培养温度22~24℃,接种量1%,培养基装量50 m L和5~7 d的培养时间有利于胞外多糖和菌丝虫草素的产生.     

高产D-甘露醇虫草菌种分离纯化的研究

本试验系用生物工程技术分离纯化虫草菌种的研究。先用组织分离法从野生虫草整体组织上分离出虫草菌种 ,再用平板稀释法将菌种纯化 ,获得一株高产D -甘露醇 (虫草酸 )的优良菌种。该菌种被鉴定为蝙蝠蛾拟青霉。使用该菌种经一级种子培养和发酵培养 ,获得含D -甘露醇 9 9% (野生虫草约 7% )、总氮 5 1% (野生虫草约 4% )的虫草菌丝体。原种斜面培养基组成 (% ) :琼脂 2、葡萄糖 2、蛋白胨 0 2、磷酸二氢钾 0 1、硫酸镁 0 0 3、麦麸 5。种子培养基组成 (% ) ;葡萄糖 2、蛋白胨 0 5、磷酸二氢钾 0 1、硫酸镁 0 0 3、麦麸 5。原种培养温度 2 3- 2 4℃、培养时间 4- 5天 ;平均稀释纯化所用稀释菌液为 10 -1、 10 -2 、 10 -3 、 10 -4 、 10 -5、 10 -6,挑菌培养温度分别为 2 3- 2 4℃、 2 8℃、 37℃。种子培养条件 :一级种子培养 :摇床转速 180r/min ,培养温度 2 4- 2 5℃ ,时间 2 - 3天 ;二级种子培养 ;摇床转速 90r/min ,培养温度 2 4- 2 5℃ ,时间 2天。发酵培养条件 ;接种量 5 - 10 % ,温度 2 4- 2 6℃ ,搅拌速度 15 0 - 180r/min ,通气培养 ,时间 5 - 6天 ,PH4 5 ,残糖量降至 0 3%。     

黑曲霉固态发酵生产果胶酶培养条件的研究

对黑曲霉HYA4固态发酵生产果胶酶的培养条件进行了研究,固态发酵培养基组分为:250 mL三角瓶中甜菜渣5 g,黄豆粉5 g,NH4H2PO4 0.4%,FeSO4·7H2O 0.05%.料水比1:2.采用此优化的培养基在培养温度36℃和初始pH自然条件下进行发酵,果胶酶酶活力可达5465.8 U/g(干曲).     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.     

Tracking data management using topology of moving object database system for moving object constrained trajectory in LBS application

Advances in wireless technologies and positioning technologies and spread of wireless devices, an interest in LBS (Location Based Service) is arising. To provide location based service, tracking data should have been stored in moving object database management system (called MODBMS) with proper policies and managed efficiently. So the methods which acquire the location information at regular time intervals then, store and manage have been studied. In this paper, we suggest tracking data management techniques using topology that is corresponding to the moving path of moving object. In our techniques, we update the MODBMS when moving object arrived at a street intersection or a curved road which is represented as the node in topology and predict the location at past and future with attribute of topology and linear function. In this technique, location data that are corresponding to the node in topology are stored, thus reduce the number of update and amount of data. Also in case predicting the location,because topology are used as well as existing location information, accuracy for prediction is increased than applying linear function or spline function.