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1.
pH值对纤维素酶制备的影响   总被引:2,自引:0,他引:2  
pH值对纤维素酶制备有很大的影响。在纤维素酶生产中以玉米秸杆为底物,若不控制pH值,任其自由发展,虽然最终酶活较高,但产酶时间较长。若用缓冲液控制pH值,最终酶活虽不及不控制pH值的产酶过程,但是产酶时间大大缩短。在产酶过程中,较高pH值有利于纤维素酶酶系中的纤维素二糖酶的诱导,较低的pH值有利于纤维素酶酶系中的C1酶和CX酶的诱导。在发酵罐产酶过程中,宜采用分段控制pH值:第一阶段pH值控制在3.5~4.0之间,第二阶段pH值控制在4.5左右。  相似文献   

2.
绿色木霉固态发酵产纤维素酶条件及酶性质的研究   总被引:4,自引:0,他引:4  
对绿色木霉 Trichoderma viride9405固态发酵产纤维素酶的条件及酶的性质进行了研究。结果表明,最适产酶条件为:秸秆粉与麸皮的比 7:3;玉米秸秆粉培养基含水量250%;花生皮粉培养基含水量150%;pH4.0~4.5;温度30℃;周期72h。在玉米秸秆粉固体培养基上,所产纤维素酶CMC活力为1386u/g,FPA活力为217u/g,棉花糖化力为325u/g。必作用的最适条件为pH4. 5~5. 0,温度 50℃;在 45℃以下, pH3. 5~6. 0之间比较稳定,室温放置半年,酶活保存率在90%以上。  相似文献   

3.
灵芝纤维素酶的纯化和性质研究   总被引:3,自引:0,他引:3  
从七种食用和药用真菌中筛选出纤维素分解能力较强的灵芝菌株(Ganodermalucidum50059),比较了几种不同碳源的固体发酵培养基对灵芝50059产内切β-葡聚糖酶(又称羧甲基纤维素酶,CMC酶)能力的影响,确定了最佳培养时间为5~6d。通过硫酸铵沉淀、凝胶过滤和离子交换层析初步提纯了灵芝的CMC酶,得到三个组分。其中一个组分在SDS-PAGE电泳中为一条带,为单亚基分子,分子量为4.5×104,等电点为3.2,以CMC-Na为底物的米氏常数(Km)为7.2g/L。该酶活力的最适温度为50℃,半致死温度约67℃。该CMC酶组分在pH3.0~6.0范围内稳定,最适pH约为2.0和4.0。  相似文献   

4.
影响固定化里氏木霉合成纤维素酶的动力学因素   总被引:3,自引:0,他引:3  
对影响固定化里氏木霉细胞合成纤维素酶的主要动力学进行了研究。以纸浆为主要碳源,采用固定化细胞,在250mL摇瓶中重复分批发酵,结果表明,在一批加料条件下,适宜于固定化细胞产酶的条件是:纸浆浓度1.5%~2.0%,C/N为8,培养液的初始pH值4.0摇瓶转速200r/min及26℃。分批添加纸浆可以减少固体底物对氧气输送和物质扩散等方面造成的不良影响,有利于增加底物的总用量,从而促进纤维素酶的合成,  相似文献   

5.
纤维素水解液对鸡腿菇纤维素酶诱导特征   总被引:2,自引:0,他引:2       下载免费PDF全文
利用正交实验法探讨纤维素不完全降解物诱导鸡腿菇产纤维素酶的特征.实验结果表明:分子结晶化程度及单体结构组成不同的纤维素原料的不完全降解物对菌种产纤维素酶的诱导有明显差异,其中结晶度低的高粱渣水解液96h可使纤维素酶FP活力高出对照组约30%;其次,水解液浓度和培养条件对菌菇产酶也有一定影响.诱导鸡腿菇产纤维素酶的最适条件为0.5%酸解高粱渣,25℃,100r/m转速,pH5.5.这可以为食用菌栽培生理的调控提供一定的基础数据.  相似文献   

6.
绿色木霉A10纤维素酶的分离纯化及理化性质研究   总被引:7,自引:0,他引:7  
绿色木霉A_(10)纤维素酶A_2的C_1酶和Cx酶被分离纯化。C_1酶的分子量为54,49,44.5kd(三个亚基),最适温度为50℃,最适pH为5.0;Cx酶的分子量为17.5kd,最适温度为55℃,最适pH为4.5。两者均为糖蛋白,富含极性氨基酸,水溶性好。  相似文献   

7.
DNS法测定纤维素酶活力最适条件研究   总被引:64,自引:1,他引:63  
鉴于纤维素酶研究和生产中,纤维素酶活力测定和酶活力单位的表示方法很不统一的现象,本文研究和分析了3,5-二硝基水杨酸法测定羧甲基纤维素酶糖化力的实验条件,如:温度、pH、酶促反应时间、DNS显色时间、波长等.实验表明:在50℃,pH4.6条件下酶促反应时间5min,DNS显色5min,于490nm处测定OD值比较适宜.  相似文献   

8.
棒曲霉UA—2木聚糖酶的蔗渣固态发酵   总被引:3,自引:0,他引:3  
棒曲霉(Aspergilusclavatus)UA-2固态发酵蔗渣产木聚酶的培养基为每克蔗渣加营养液5ml,初始pH8.5.营养液的适宜组成为每升含:NH4NO310g、K2HPO46g、MgSO4·7H2O0.75g、CaCl20.75g、FeSO4·7H2O11.25mg、MnSO4·H2O3.75mg、ZnSO43.0mg、CoCl24.5mg.在上述培养基中接入其湿重10%(W/W)的新鲜的UA-2种子曲,28℃培养108h,其木聚糖酶,滤纸降解酶和羧甲基纤维素酶的活力分别为2695.6、28.5和42.7u/g蔗渣.酶反应的最适pH5.0,最适温度50℃;在pH4.0~11.0内酶活性十分稳定.50℃保温1h,酶活剩余55%,8℃下放置23d,活力几乎不变.磷酸盐,半胱氨酸对酶有激活作用,EDTA和对氯汞本甲酸对酶有抑制作用  相似文献   

9.
以里氏木霉(Trichoderma reesei)Rut C-30为产酶菌,研究了不同初始pH值对木聚糖酶和纤维素酶合成的影响。结果表明,初始pH较低有利于纤维素酶的合成,初始pH值高则延长了产木聚糖酶的时间,且强烈抑制纤维素酶的合成,致使木聚糖酶活与纤维素酶活的比值提高,从而有利于选择性合成木聚糖酶。初始pH值分别为4.0、4.4、4.8、5.4、6.0时,培养72h,木聚糖酶活与纤维素酶活的比值分别为259、327、425、865、1016。随着初始pH值的增加,里氏木霉对培养液中还原糖的消耗能力在降低,初始pH值在4.0-4.8范围内,培养48h时还原糖浓度到最低,并且维持在低浓度的水平上。当初始pH值在5.4-6.5范围内,培养72h时还原糖浓度才达到最低,并且培养液中还原糖浓度较高,初始pH值提高到6.5时,培养液中还原糖浓度维持在较高水平上,抑制了木聚糖酶和纤维素酶的合成。  相似文献   

10.
产黄纤维单胞菌纤维素酶的培养条件   总被引:3,自引:0,他引:3  
对产黄纤维单胞菌所产纤维素酶的条件和天然纤维素能力的利用进行了研究,认为该菌产酶最适起始PH为6.0最适产酶温度为25℃,8-16h为产酶高峰期;CMCNa,酪蛋白是该菌最好的碳氮源,高浓度的葡萄糖抑制纤维素酶的形成,而纤维二糖是该菌的较好碳源和诱导剂。  相似文献   

11.
Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.  相似文献   

12.
何延凌 《科技信息》2008,(4):258-258
Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women's language from these perspectives: pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women's language, more attention should be paid to language use in social context. What's more, the linguistic phenomena in a speaking community can be understood more thoroughly.  相似文献   

13.
The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3~4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰~ -7.8‰, PDB) and matrix dolomites (-6.6‰~ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084~0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078~0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.  相似文献   

14.
There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.  相似文献   

15.
理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。  相似文献   

16.
王慧 《科技信息》2008,(10):240-240
Wuthering Heights, Emily Bronte's only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.  相似文献   

17.
The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.  相似文献   

18.
In the 19th century the society was controlled by men, and women were just appendants of them, they had not any rights and freedom. But Jane was an exception, she showed some characteristics of early feminist. Jane showed her characteristics of feminism in three aspects: rebellion, equality, and independence. These characteristics were helpful to her success, and feminism is the only way out for women of that time.  相似文献   

19.
As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.  相似文献   

20.
1 Rise of studies on climate change's effects on biodiversity
Until the 1980s, climate change and biodiversity were studied as two independent disciplines for more than a century. In 1992, the Ecological Society of America's annual report named climate change, biodiversity, and the sustainable ecological system as the three major global environmental issues of the twenty-first century [1].  相似文献   

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