荧光素及同位素标记LZF—1探针用于大熊猫亲子鉴定的DN…

以大熊猎４个家系，３０次个体的被毛，皮肤和福尔马林固定的肝组织作材料，用荧光素和同位素分别标记寡核苷酸探针ＬＺＦ－１，作ＤＮＡ指纹图谱检测。结果表明，无论２雄配１雌，３雄配１雌，甚至４雄配１雌，受配雌兽所生子代的ＤＮＡ指纹图谱条带，均来自父亲，母亲及你母双亲，没有新的条带出现，准确认定了４个家系中６只子代个体的真父，排除了非父。     

大熊猫亲子鉴定：DNA指纹技术的应用

以大熊猫１０个家系，２０只个体的被毛为材料，辅以血液，精液，作ＤＮＡ指纹图谱的对比分析，结果表明：在同一大熊猫个体的被毛和血液，被毛和精液，血液与精液组织检测，其ＤＮＡ指纹图谱完全一致；大熊猫多雄配一雌及一雄配一雄，所产任一代个体的ＤＮＡ指纹图谱中，其杂交带都分别来自父亲和母亲，或父母亲，没有新带出现，准确无误地确定了１０个家系子代的真父，排除了非父；三只大熊猫双胞胎中６只子代个体的ＤＮＡ指纹图谱     

用M13DNA探针检测小鼠DNA指纹

用ＨｉｎｆＩ酶切，随机引物法标记的Ｍ１３ｍｐ１８ＤＮＡ作探针，对无关小鼠个体及一个小鼠家系三代进行了ＤＮＡ指纹分析，无关个体平均每个个体得到１４条谱带（＞４ｋｂ）。无关个体具有相同指纹图概率为９×１０￣（－８），显示高度个体材异性，对小鼠家系内后代与亲本图谱进行了比较，表明图谱特征按孟德尔方式遗传。对家系内个体间遗传相似度Ｄ进行了计算，亲缘关系近则Ｄ值高，据此对本技术在动物个体识别，亲缘关系分析等方面的应用进行了讨论。     

拉萨地区小云雀（Alaudagulaglainopinata）遗传多样性的DNA指纹图分析

ＤＮＡ指纹探针ＬＺＦ－Ｉ用于拉萨地区小云雀（Ａｌａｕｄａｇｕｌａｇｌａｉｎｏｐｉｎａｔａ）遗传多样性的ＤＮＡ指纹图研究，结果表明：（１）同一个体的肝、肾、心、肌肉和十二指肠的ＤＮＡ指纹图谱完全相同，无组织特异性，表明鸟类的任何器官组织都能作为ＤＮＡ指纹的分析材料．（２）检测随机个体ＤＮＡ指纹图的平均杂交谱带数为２１．８条，相似系数为０．０９３，平均杂合率为８６．３％．（３）在随机个体检测中，ＬＺＦ－Ｉ探针的鉴别机率为２．１３×１０－２３，ＤＮＡ指纹图具有高度的个体特异性，群体基因组表现出高度的多态性．     

乌鸡的性状遗传率,遗传相关和杂种优势

本文以乌鸡４个品种作为全群，从中抽出５只雄亲和５套雌系进行巢式交配设计，测定了子代出雏期、１月龄、２月龄、３月龄的体重和胫长．遗传分析表明：（１）雌亲分量遗传率明显高于雄亲分量遗传率，说明群体存在明显的非加性变异；（２）各发育期体重和胫长的遗传相关和表型相关一般为正相关且较大，从而可根据胫长间接选择体重；（３）体重杂种优势变动为－７％～３２％；（４）在强优势Ｆ１组合，１月龄相对生长速度明显高于其亲本组合的，但２月龄的恰相反，这为生产缩短Ｆ１的饲养周期有利．最后讨论了雌亲分量遗传率高于雄亲分量遗传分量和杂种优势产生的原因．     

拉萨地区小云雀（Alaudagulaglainopinata）遗传多样性…

ＤＮＡ指纹探针ＬＺＦ－用于拉萨地区小云雀（Ａｌａｕｄａｇｕｌａｇｌａｉｎｏｐｉｎａｔａ）遗传多样的ＤＮＡ指纹图研究，结果表明：（１）同一步体的肝、肾、心肌肉和十二指肠的ＤＤＡ指纹图谱完全相同，无组织特异性，表明乌类的任何器官组织都能作为ＤＮＡ指纹的分析材料。（２）检测随机个体ＤＮＡ指纹图的平均杂交谱带数为２１．８条，相似系数为０．０９３，平均杂合率为８６．３％。（３）在随机个体检测中，ＬＺＦ－Ｉ探     

应用cDNA检测RFLPs和核酸杂交剂量诊断DMD／BMD基因携带者的研究

用ｄｙｓｔｒｏｐｈｉｎｃＤＮＡ检出ＢｇｌⅡ和ＸｂａⅠＲＦＬＰｓ，结合ＤＮＡ杂交剂量分析法，检测了申请作了ＤＭＤ／ＢＭＤ杂合子鉴定的８个家系６９个人的基因型，通过８个ＲＦＬＰｓ的基因内连锁分析和基因剂量分析，１１名女性被诊断为ＤＭＤ基因突变携带者，４名女性为可能携带者，１２名女性被确认为正常个体，３名未到出现ＤＭＤ临床症状年龄的男孩被诊断为正常个体，此外，在４０名子代中发现２名女性有基因内重组，１名     

大熊猫雄性生殖器害元素EDS分析

应用扫描电镜及ｘ－射线能谱仪，对大熊猫雄性生殖器官的曲细精管４个区域、附睾头、附睾体、附睾尾、输精管及输精管壶腹各一个区域的１５种元素进行了测定，其中，常量元素６种，微量元素９种，依据典型图谱对各采谱区域内的元素含量变化作了定量计算，以Ｚｎ为基准对几种与生殖机能密切相关的元素作了对比分析，其中配种能力好精子数量多，精液质量优良，使雌兽受孕产仔率高的“强强“，其微量元素Ｚｎ、Ｍｎ，Ｆｅ，Ｃｕ，Ｃａ，     

兰属植物DNA指纹图的研究

采用ＬＺＦ－１寡核苷酸探针和限制性内切酶Ｈｉｎｆ１,检测兰属２３样株和石斛属１样株的ＤＮＡ酶解片段,获得了清晰易读的由６－１５条带组成有特异性的遗传指纹图谱。表明：（１）ＬＺＦ－１探针能与兰属及石斛属植物基因组ＤＮＡ中的酶解片段中简单重复序列形成杂交分子,证实近缘植物基因组中存在同源ＤＮＡ片段;（２）在介于１－２４ｋｂ的谱带中,大红朱砂（Ａ）１５条,３株红蝉（Ｂ）皆１２条,Ａ＊ＢＦ１２ｎ＝４０两株     

大熊猫雄性生殖器官元素EDS分析

应用扫描电镜及ｘ－射线能谱仪，对大熊猫雄性生殖器官的曲细精管４个区域、附睾头、附睾体、附睾尾、输精管及输精管壶腹各一个区域的１５种元素进行了测定，其中，常量元素６种，微量元素９种。依据典型图谱对各采谱区域内的元素含量变化作了定量计算。以Ｚｎ为基准对几种与生殖机能密切相关的元素作了对比分析。其中配种能力好精子数量多，精液质量优良，使雌兽受孕产仔率高的“强强”，其微量元素Ｚｎ、Ｍｎ、Ｆｅ、Ｃｕ、ｃａ、Ｋ的相对含量较高，特别是Ｚｎ的含量显著增高（Ｐ＜０．０５）。     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.