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1.
用PCR方法检测了采集于不同地域的稻田飞虱共生菌Wolbachia的感染,发现灰飞虱Laodelphax striatellus,褐飞虱Nileparvata lugens,白背飞虱Sogatella furcifera 为Wolbachia所感染。克隆了编码Wolbachia外膜蛋白质的wsp基因并进行了序列测定,对wsp的RFLP分析证实了这些飞虱为单一Wolbachia感染,还发现一种能同时  相似文献   

2.
灰飞虱Wolbachia群体生物学的遗传特性研究   总被引:6,自引:0,他引:6  
Wolbachia是存在于多种昆虫生殖器官的一类共生菌,它可能在宿 主群体中引起细菌质不亲和性而给宿 主带来生殖优势。对灰飞虱体内的Wolbachiafjtfdgpw,v gm pgcfmw qnnkudyjr do nui  相似文献   

3.
不同种群灰飞虱(Laodelphax striatellus)的RAPD分析   总被引:9,自引:0,他引:9  
用RAPD技术对9个不同的灰飞虱地理种群的DNA多态性进行了研究。按Neighbor-joining聚类法构建了这9个种群灰飞虱的分子系统树。该系统树分为2簇,簇1包括我国北京、福建、海南、辽宁、四川、上海、云南地区的灰飞虱种群,簇2包括宁夏地区和日本东京地区的2个灰飞虱种群。研究表明,不同地区灰飞虱种群的遗传距离与其地理距离不呈相关性。  相似文献   

4.
用PCR方法检测了多种果蝇中共生菌Wobachia的感染,发现,Drosophila auraria复合以及采集于北京地区的Drosophila simulans为Wolbachia所感染,RFLP分析证实为单一感染,克隆了编码Wolbachia外膜蛋白质的wsp基因并进行了序列测定。  相似文献   

5.
Wolbachia在Drosophila auraria复合种和Drosophila simulans中的感染   总被引:2,自引:0,他引:2  
用 P C R 方法检测了多种果蝇中共生菌 W olbachia 的感染,发现 Drosophila auraria 复合种以及采集于北京地区的 Drosophila sim ulans为 Wolbachia 所感染, R F L P分析证实为单一感染.克隆了编码 W olbachia 外膜蛋白质的 w sp 基因并进行了序列测定.同时比较了 Drosophila auraria 复合种内 4 个种和采集于北京及美国加州的 Drosophila sim ulans 的线粒体的细胞色素氧化酶 2 亚基基因的部分序列. 进而讨论了 Wolbachia 对 Drosophila auraria 复合种成员之间进化关系的影响.  相似文献   

6.
近年来,由于小麦和玉米病毒病在河北省的蔓延流行,因而对于传毒媒介昆虫——灰飞虱的研究也逐渐被重视起来。虽然主要传毒昆虫是灰飞虱,但在石家庄地区还有一些其它种类飞虱与灰飞虱混生,因此在研究灰飞虱发生规律的过程中,必然要涉及与灰飞虱混生的其它种类飞虱。为此,今年我们对石家庄地区常见飞虱的发生概况进行了初步的调查。  相似文献   

7.
在我国首次报道卡内里虱蝇(Pseudolynchiacanaiensis)的染色体核型及第一次减数分裂的形态特征.该虱蝇二倍体细胞的染色体数目2n=6,由2对中部着丝粒的常染色体和1对性染色体组成。x染色体为亚中部着丝粒,Y染色体为端部着丝粒,且x染色体长于Y,本文还对该虱蝇的核型及其演化进行了比较分析.  相似文献   

8.
[目的]明确西昌地区主要粉虱种类及寄主植物 为有效预防粉虱的传播蔓延提供理论依据 [方法]对西昌地区主要 粉虱种类及其寄主进行调查采集 通过制作永久玻片 观察测量粉虱伪蛹的典型特征 根据其形态学特征鉴定粉虱种类 [结 果]在西昌地区 14 科 22 种植物上分离鉴定出 6 种粉虱 即黑刺粉虱 Aleurocanthus spiniferus、棒粉虱属未鉴定粉虱 Aleuroclava sp.、烟粉虱 Bemisia tabaci、孟加拉皮粉虱 Pealius bengalensis、灰粉虱 Siphoninus phillyreae、温室白粉虱 Trialeurodes vaporariorum 其中烟粉虱为害作物占比最多、分布最广泛 是西昌地区为害最严重的粉虱种类 [结论]西昌地区 14 科 22 种植物上有 6 属 6 种粉虱 其中烟粉虱为优势种  相似文献   

9.
本文描述了贵州黔东南中,下凯里组下部Redlichia的两个新亚各Redlichia及R.(R.)和一个未定种Redlichia(Redlichia)sp.,并讨论了Redkichia在中下寒武统界线划分对比的作用及意义。  相似文献   

10.
工绿飞虱Saccharosydne procerus(matsumura)是茭白的主要害虫之一,我地各茭区均有发生,部分地区为害较重,1986-1988年,我们对长绿飞虱进行了初步研究,结果表明,长绿飞虱在孝感地区一年发生4人代,有世代重叠现象,以7,8月间的1,2代为害最烈,茭白遭长绿飞虱为害后,呈现不规则的淡褐色至棕褐色斑点,严重时螯叶枯死,长绿飞虱天敌种类较多,对长绿飞虱都有一定的[控制作用,经药剂防治试验表明:2%叶蝉散粉杀虫效果最好,其次是50%混灭成乳剂和40%氧化乐果。  相似文献   

11.
Wolbachia在灰飞虱体内的分布   总被引:9,自引:0,他引:9  
灰飞虱(Laodelphax striatellus)能传播水稻条纹叶枯病,是我国主要的水稻害虫,有些灰飞虱体内含有细胞内共生菌-Wolbachia。Wolbachia是一种细胞质遗传的细胞内共生菌,这类细菌改变了宿主的生殖行为,引起细胞质不相容(CI),雌性化及孤雌生殖等现象,导致带有Wolbachia的宿主具有生殖优势。采用PCR和Western杂交的方法分析Wolbachia的灰飞虱体内不同组织的分布状况,发现灰飞虱体内Wolbachia除存在于生殖组织外,还广泛分布在头、胸、腹、唾液腺、消化道等非生殖组织中。这种广泛的分布状态说明Wolbachia是在昆虫体内引入、表达与传播外源基因的良好媒介。  相似文献   

12.
Wolbachia is a common maternally inherited bacterial symbiont able to induce crossing sterilities known as cytoplasmic incompatibility (CI) in insects. Wolbachia-modified sperm are unable to complete fertilization of uninfected ova, but a rescue function allows infected eggs to develop normally. By providing a reproductive advantage to infected females, Wolbachia can rapidly invade uninfected populations, and this could provide a mechanism for driving transgenes through pest populations. CI can also occur between Wolbachia-infected populations and is usually associated with the presence of different Wolbachia strains. In the Culex pipiens mosquito group (including the filariasis vector C. quinquefasciatus) a very unusual degree of complexity of Wolbachia-induced crossing-types has been reported, with partial or complete CI that can be unidirectional or bidirectional, yet no Wolbachia strain variation was found. Here we show variation between incompatible Culex strains in two Wolbachia ankyrin repeat-encoding genes associated with a prophage region, one of which is sex-specifically expressed in some strains, and also a direct effect of the host nuclear genome on CI rescue.  相似文献   

13.
RAPD analysis is used to assay the population genetic structure and genetic differentiation of pufferfish T.rubripes and T.pseudommus.One hundred and twenty fragments are aplified with 21 random sequence 10-mer primers.The proportion of polymorphic fragments of T.rubripes populations from the coast of China(TRC),the coast of Japan(TRJ),general population of T.rubripes both from China and Japan(TRCJ),and the population of T.pseudommus are 31.7%,33.3%,35.0%and 39.2% respectively.The mean expected heterozygosities of the four populations are 0.116,0.125,0.126,and 0.144,respectively.Low genetic distances(ranging from 0.0118 to 0.0309)and Fst estimates(0.020 to 0.024) among the populations indicated that there is no significant differentiation between T.rubripes and T.pseudommus and suggested that only one species is involved.  相似文献   

14.
中国绢藓属Entodon植物的地理分布   总被引:1,自引:0,他引:1  
本文依据编写全国志所收集的标本和资料 ,分析绢藓属EntodonC .Muell.在中国的分布特点 ,提出我国绢藓属主要分布中心在西南部 ,由此向东逐渐扩展。黄柄亚属subgenusEntodon主要分布长江以南各省 ,绢藓亚属subgenusErythropus (Broth .)Miz .则广泛分布中国各个地区  相似文献   

15.
采用线粒体DNA细胞色素c氧化酶亚单位I(COI基因)部分序列,对我国西南地区15个地点共计240个云南切梢小蠹个体进行扩增和测序.研究发现,云南切梢小蠹不同地理种群间在线粒体基因组水平上具有不同程度的多样性,位于云南省中部地区的采样点遗传多样性较为丰富,而西北的丽江和宁蒗遗传多样性比较单一,四川的3个采样地在地理上很近,多样性也较为丰富;研究证实,云南切梢小蠹为单起源,源于云南中部,随后向四周扩散分布;云南切梢小蠹的分布与云南松分布息息相关,二者相辅相成,没有云南松的地方亦没有云南切梢小蠹.  相似文献   

16.
Genetic diversity of Tricholoma matsutake in Yunnan Province   总被引:3,自引:0,他引:3  
To investigate the genetic diversity of Tricholoma matsutake,we studied ITS and IGS1 sequences and PCR polymorphism of a retrotransposon in 56 fruit bodies collected from 13 counties of 9 regions in Yunnan Province. We found one and three haplotypes based on ITS and IGS1 sequences,respectively. Moreover,there was no significant difference in PCR polymorphism of the retrotransposon among different populations. Compared with Jilin Province(China) and Japanese populations,although Yun-nan was highly homogenous to Japanese populations,low genetic diversity of T. matsutake in Yunnan did not support the view that this species originated from Yunnan.  相似文献   

17.
Genetic manipulations of insect populations for pest control have been advocated for some time, but there are few cases where manipulated individuals have been released in the field and no cases where they have successfully invaded target populations. Population transformation using the intracellular bacterium Wolbachia is particularly attractive because this maternally-inherited agent provides a powerful mechanism to invade natural populations through cytoplasmic incompatibility. When Wolbachia are introduced into mosquitoes, they interfere with pathogen transmission and influence key life history traits such as lifespan. Here we describe how the wMel Wolbachia infection, introduced into the dengue vector Aedes aegypti from Drosophila melanogaster, successfully invaded two natural A. aegypti populations in Australia, reaching near-fixation in a few months following releases of wMel-infected A. aegypti adults. Models with plausible parameter values indicate that Wolbachia-infected mosquitoes suffered relatively small fitness costs, leading to an unstable equilibrium frequency <30% that must be exceeded for invasion. These findings demonstrate that Wolbachia-based strategies can be deployed as a practical approach to dengue suppression with potential for area-wide implementation.  相似文献   

18.
调查了104个畸形外囊菌(Taphrina deformans(Berk)Tul)的发生地,并通过基因遗传算法(GARP)模型对畸形外囊菌在中国的适生情况进行了预测.实验中采用受试者工作特征曲线(简称ROC曲线)中的曲线下面积(AUC)的大小评估模型预测的准确性,所得AUC值为0.89,表明诊断价值较高.预测结果表明畸形外囊菌在中国的适生区主要分布在东部地区:河北南部、山西南部、广东南部、山东全部、河南全部、安徽全部、江苏全部、上海全部、浙江全部、海南全部、福建大部分、江西大部分、台湾西部沿海、云南东部、湖北东部和湖南东部.  相似文献   

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