CHX抗栓注射液对小鼠脑缺氧保护作用研究

目的通过CHX抗栓注射液对小鼠断头存活时间和常压耐缺氧能力的影响,观察该药对小鼠脑组织急性缺氧的保护作用.方法给小鼠腹腔注射CHX抗栓注射液每天2.5 nmg/kg,5 mg/kg,7.5 mg/kg,连续7d,实验采用小白鼠断头法和小鼠常压缺氧模型.结果不同浓度的CHX抗栓注射液,均可明显延长小白鼠断头存活时间和常压缺氧存活时间,与对照组相比,差异非常显著.结论 CHX抗栓注射对小白鼠脑缺氧具有保护作用.     

雪莲对兔“热毒血瘀证”模型血液凝固性及血小板免疫粘附功能的影响

检测血液凝固性和血小板免疫粘附功能是研究血瘀证实质和活血化瘀药理作用的重要手段。为了探讨雪莲活血化瘀的药效及作用机制,本实验对沙门氏菌性“热毒血瘀”模型兔给药前后的血小板数、血小板电泳速率、血小板聚集性、体外血栓形成、血浆纤维蛋白原含量、优球蛋白溶解时间及血小板免疫粘附功能进行了检测。证明雪莲对二磷酸腺苷(ADP)诱导的正常兔和“热毒血瘀”模型元的血小板聚集性具有很强的抑制作用,其抑制率分别为21.1±2.9％(±Sx)和25.6±2.7％(±Sx),且对模型兔血小板聚集性的抑制作用要比对正常兔的强(P<0.01);雪莲对亢进的血小板免疫粘附功能具有抑制作用。此外,雪莲还具有抗体外血栓形成及抗机体纤溶活性降低的作用。但雪莲对血小板的表面电荷无影响,对血小板的生成也无明显促进作用。结果表明,雪莲的活血化瘀作用机制与其促进机体纤溶活性和降低血液凝固性有着密切关系。     

CHX抗栓注射液对实验性大鼠脑缺血的保护作用

目的：研究CHX抗栓注射液对实验性大鼠急性不完全性脑缺血的保护作用．方法：采用结扎双侧颈总动脉法，造成大鼠急性不完全性脑缺血模型．观测药物对脑含水量、毛细血管通透性和脑指数的影响．结果：不同质量分数(5mg／kg，10mg／kg)的CHX抗栓注射液可明显降低治疗组大鼠的脑含水量、毛细血管通透性和脑指数．结论：CHX抗栓注射液对大鼠急性不完全性脑缺血有一定的保护作用．     

重组人尿激酶原药效学研究

利用人血浆及其产生的血栓凝块 ,在试管中模拟纤溶酶原激活剂在体内引起血栓溶解的过程及麻醉开胸犬电刺激冠脉左旋支引起的冠脉血栓形成模型评价重组人尿激酶原的溶栓活性 ,并与尿激酶进行比较 .InVitro实验结果表明 :2 4 0IU /mL是 prouk血纤维蛋白专一的最大浓度 ,低浓度的重组prouk比天然 prouk具有更高的溶栓能力 ,这可能与其非糖基化结构有关 .当重组prouk浓度小于 1μg/mL时 ,它在血浆中几乎不降解任何纤维蛋白原 .犬静脉给予重组人尿激酶原 9× 10 4 、4 .5× 10 4 、2 .2 5× 10 4 IU·kg- 1 对冠脉血栓产生显著的溶栓效果 ,栓塞冠脉血管很快出现再通 ,残存血栓较溶剂对照分别减少了 6 9.2 %、5 7.0 %、4 3.1% ;心肌梗死范围明显缩小 ;与等剂量尿激酶溶栓作用相似 .血浆优球蛋白溶解时间明显缩短 ;溶栓不伴有明显的血浆纤维蛋白原降解 ,而尿激酶溶栓的同时 ,纤维蛋白原明显降低 .除高剂量组个别动物外 ,对伤口出血量增加出血时间无明显影响 .而等剂量的尿激酶组伤口出血量及出血时间明显延长     

甘糖酯对大鼠尿激酶型纤溶酶原激活物活性的诱导作用

为了研究甘糖酯对大鼠体内尿激酶型纤溶酶原激活物（urokinase Plasminogen Activator,uPA)活性的影响,采用溶解圈中和抑制法和发色底物法检测了大鼠口服甘糖酯后体内uPA活性的变化情况。结果表明：甘糖酯可提高大鼠体内血液的纤溶活性,主要表现为uPA活性升高。以不同剂量的甘糖酯连续喂药10d后,发现喂药组大鼠uPA活性明显高于对照组,喂药组大鼠血浆uPA活性升高同喂药剂量正相关,在0－100mg/kd的剂量范围内,随喂药剂量增加而升高。提示在一定的剂量范围内甘糖酯可提高大鼠体内uPA活性,激活纤溶系统,提高血液纤溶活性,表现出良好的抗栓作用。     

用沙门氏菌复制兔“热毒血瘀证”病理模型的研究

为了探讨温病血瘀的形成机理,采用肌注沙门氏菌的方法,复制了兔“热毒血瘀证”模型,并对模型兔的血液流变性、血液凝固性等方面进行了研究。结果表明:全血粘度在切速为230s~(-1),115s~(-1)和23s~(-1)下分别为0.004684±0.000455pa·s,0.005093±0.000515pa·s和0.00678±0.000789pa·s;全血还原粘度为0.0147±0.00076 pa·s/％;血浆粘度为0.008185±0.000334pa·s;血沉为16.9±3.0mm/h;血沉k值为35.7±8.30,均显著高于正常对照组(p<0.01)。红细胞压积为31.9±2.6％;红细胞电泳速率为3.5±0.31μm/s,均显著低于正常对照组(p<0.01),血小板聚集性为58.21±4.05％;血小板免疫粘附功能为1.09±0.33;血浆纤维蛋白原含量为1.47±0.28g％;优球蛋白溶解时间为37.30±11.14h,均显著增加(p<0.001),血小板数为40.06±3.24万/mm~3;血小板电泳速率为4.71±0.34μm/s均显著降低(p<0.001)体外血栓形成的长度为57.22±9.07mm;湿重215.93±10.85mg;干重61.02±9.48mg,均显著高于正常对照组(p<0.01)。临床呈现出高热、呼吸心率加速、体表血管充血或有瘀血斑、口色瘀紫色及内脏组织器官的病理损伤。实验结果认为以沙门氏茵接种于兔所复制的病理模型符合中医、中兽医有关热毒和血瘀的理论,当属“热毒血瘀证”模型。     

药物致横纹肌溶解的动物实验研究

目的 探索他汀类药物、贝特类药物联合给药在大鼠、小鼠中导致横纹肌溶解的情况,为后续进行哈夫病等 病因不明的横纹肌溶解综合征研究提供参考。 方法 采用辛伐他汀、吉非罗齐联合给药及单独给药对 Wistar 大鼠 (5 周龄) 、ICR 小鼠(5 周龄)连续灌胃给药 14 d,测定血清肌酸激酶( CK) 、肌红蛋白( MYO) 、谷草转氨酶( AST) 、谷 丙转氨酶( ALT)的含量,对其肌肉损伤指标变化趋势进行分析。 在实验结束后取后肢肌肉组织进行苏木精-伊红 ( HE)染色病理切片检查。 结果 辛伐他汀、吉非罗齐连续联合给药 10 d 后 Wistar 大鼠 CK、ALT、AST 升高,与给药 前及对照组对比差异显著( P<0. 05) ,肌肉组织病理检查显示肌纤维坏死。 ICR 小鼠给药前后,与对照组相比,各生 化指标无显著性差异。 结论 Wistar 大鼠在药物作用下可发生横纹肌溶解,提示在后续进行哈夫病等不明原因横 纹肌溶解动物实验研究时,可考虑使用 Wistar 大鼠。     

速眠新在大鼠麻醉中的应用分析

分析不同剂量速眠新麻醉大鼠的效果。将 18只大鼠分成三组 ,分别按每千克体重 0 4、0 8、1 2mL速眠新进行肌肉注射麻醉。结果给予速眠新后分别在 ( 7± 1)、( 5± 1)和 ( 4± 1)min大鼠进入麻醉状态 ,麻醉维持时间分别为 ( 31± 6 )、( 89± 11)和 ( 111± 10 )min。大鼠在麻醉维持期间呼吸频率分别为 ( 6 2± 12 )、( 6 0± 11)和 ( 70± 15 )次 /min ,心跳频率分别为 ( 175± 16 )、( 16 5± 12 )和 ( 16 5± 13)次 /min。速眠新麻醉大鼠的效果满意 ,适宜剂量为每千克体重肌肉注射 0 4mL～ 0 8mL。     

野木瓜注射液阻滞神经传导

野木瓜注射液可阻滞神经传导和解髓鞘,在临床上有显著的镇痛作用,通过有效成分的分离、提取和初步药理研究,发现其中皂甙有神经传导阻滞作用。神经干外敷0.3％皂甙后,60min起效,使大鼠隐神经复合动作电位A成分下降为给药前的0.22±0.28(n=5),80min时为0.03±0.05,P<0.001。     

灯盏花素葡萄糖注射液的药效学研究

目的验证灯盏花素葡萄糖注射液的临床前药效学作用;方法选择不同的药理学指标对灯盏花素葡萄糖注射液的药效学作用进行评价;结果灯盏花素葡萄糖注射液对实验性大鼠急性脑缺血具有显著的保护作用,可使大鼠血液中纤维蛋白溶解酶活性明显增加,对大鼠血液血栓形成具有明显的抑制作用,可使小鼠血液凝血时间显著性延长,对小鼠肠系膜微循环具有明显的改善作用,具有明显抑制血小板聚集的功能,亦可显著性降低家兔脑血管阻力,改善脑循环;结论试验结果证明了灯盏花素葡萄糖注射液具有良好的活血化瘀作用,对改善脑血循环、增加脑血流量、降低脑血管阻力、急性脑缺血改善、增加血液纤溶酶活性、防治血液血栓形成等功效显著;讨论研究结果与文献相关报道基本一致,为其临床广泛应用提供了有力的科学依据.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Wuhan University Journal of Natural Sciences TOTAL CONTENTS

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