玉米叶片硝酸还原酶活性测定条件的优化

以玉米品种农大108幼苗为材料,研究了温度、pH值、叶位及一天中的不同时段对硝酸还原酶(NR)活性的影响,同时对光照及三氯乙酸(TCA)对NR的影响效应进行了评价。结果表明,玉米硝酸还原酶在24℃,pH 7.5下的活力最高;不同叶位,刚刚展开的叶片(心2叶)活性极显著高于其它叶位;NR在一天中的不同时段活性不同,以15:00的活性最高。不同测定条件下,与照光处理相比,黑暗条件下的酶活性显著提高。此外,加入变性剂三氯乙酸使玉米叶片内的NR活性明显减低。     

植物硝酸还原酶的体内测定法

硝酸还原酶(NR)是植物氮素代谢中的关键酶,可作为植物育种和营养诊断的生化指标,近五年来我国在稻、麦、玉米等作物上,亦已揭示出NR活力与作物耐肥性呈负相关这一规律,因此测定NR活力的大小,能够反应作物耐肥性能的弱或强。硝酸还原酶的测定方法有体外法(in Vitro)及体内法两种,体外法测定的数据比较精     

优质油菜营养生理研究

优质(低芥酸、低硫甙)油莱与普通油莱的营养生理特征有明显不同,其植株的可溶性总糖及含氮量在苗前期与成熟期差异很小,到越冬期、苔期和花期则相差很大,出现“梭形过程”。优质油莱在这一过程中表现“糖高氮低”,其硝态氮含量、硝酸还原酶(NR)活力及光合作用速率等生理特征均表现较弱,对氮肥反映较敏感,每生产100公斤菜籽的需氮量相对较少。     

珍珠梅和榆叶梅茎直径微变化及其原因

晴好天气下,测定2种蔷薇科植物珍珠梅(Sorbaria sorbifolia A.Br)和榆叶梅(Prunus triloba Lindl.)茎直径、木质部直径、韧皮部直径、木质部含水量、叶水分亏缺和蒸腾速率日变化.结果表明,珍珠梅和榆叶梅茎直径呈现白天收缩,傍晚、夜间恢复或者膨胀的变化过程.珍珠梅和榆叶梅木质部直径变化趋势与茎直径变化趋势一致,而韧皮部直径无明显变化;相关分析表明木质部直径变化与茎直径变化呈现极显著正相关,说明木质部直径的变化是引起茎直径变化的主要原因.珍珠梅和榆叶梅木质部含水量与其木质部直径呈现极显著正相关;茎直径与叶水分亏缺呈现极显著负相关性,与水分代谢有密切关系.说明珍珠梅和榆叶梅的茎直径变化是由体内水分平衡决定的.     

甜菜硝酸还原酶活性分析及基因片段的克隆

为了明确缺氮条件下甜菜NR的活性,用活体测定法检测了经缺氮胁迫不同时间后甜菜叶中NR活性．结果表明,随着缺氮处理时间的延长,甜菜叶中的NR活性逐渐降低,在缺氯处理1h后,其活性下降较快．用50mmol／1．KNO3溶液处理的甜菜幼苗总RNA,通过RT—PCR分离得到了硝酸还原酶基因片段,长度为471bp,Blast分析表明,其与Genbank中硝酸还原酶基因部分序列具有高度同源性．     

内蒙古典型草原常见种营养元素生殖分配动态规律研究

测定了锡林郭勒草原中12种常见植物种群在不同的物侯期（营养、花蕾、结实）不同器官（茎、叶、花、果）中6种营养元素（C、N、P、K、Ca、Mg）的含量，以其探索随物侯期的变化，营养元素在生殖分配上的动态规律。结果如下：①茎中的营养元素不随物侯期的变化而变化；营养元素含量在营养期叶中既与不同物侯期的叶有明显区别，又与同一物侯期的不同器官（营养期茎）有明显差异。②不同植物种群的6种营养元素生殖分配只在营养物侯内表现一致，叶中分配比例明显大于茎中的，在茎中占20％－35％，叶中占65％－80％。     

小麦各生育期硝酸还原酶的活性及其与作物耐肥性的关系

作物根系吸收硝态氮后,运往叫片先被还原成氨态氮,然后合成为有机氮。在硝态氮还原过程中,硝酸还原酶(NR)起着调控作用。有关作物耐肥性的研究亦已阐明,作物对氮素的反应特性与NR活性存在着负相关,不同品种间NR活力的差异,主要是由于体内酶的诱导合成水平不同而造成。由于NR是一种诱导酶,在植物体内极不稳定,诸如光强、温度、pH、CO_2、O_2分压、水势、氮的供应水平等等因素,都会导致NR活性的变化和波     

不同耐肥性的小麦品种幼苗硝酸还原酶、过氧化物酶活力及同功酶的研究

在不同耐肥性的6个小麦品种幼苗中,硝酸还原酶(NR)活力差异显著,耐肥性弱的品种NR活力高,活力是负相关关系。过氧化物酶活力,耐肥性强的高于耐肥性弱的。根据聚丙烯酰胺凝胶电泳分离出的过氧化物酶的同工酶谱,耐肥性强的品种均有8条酶带,且着色深,耐肥性弱的品种则有7条,着色较浅。此外通过根系活力测定,耐肥性强的低于耐肥性弱的品种,而叶绿素含量则高于耐肥性弱的品种。     

水稻幼苗硝酸还原酶与叶绿素和硝态氮的含量及铵态氮吸收的关系

三种蛋白质含量不同的水稻品种,其幼苗的硝酸还原酶的活力不同,叶绿素和硝态氮的含量也不同,凡叶绿素和硝态氮含量亭的其硝酸还原酶的活力也高,凡叶绿素和硝态氮含量低的其硝酸还原酶的活力也低。华03的谷粒蛋白质含量高。其幼苗根系对铵态氮的吸收能力也强。上述结果说明,蛋白质含量不同的水稻品种,其生理生化差异在幼苗期已表现对氮的吸收和利用不同。     

宁夏区内5种牧草可溶性糖和淀粉含量的研究

采用蒽酮比色法对豆科5种牧草营养期组织中可溶性糖和淀粉的含量进行了测定及分析.结果表明,在营 养期,同一牧草的不同器官内可溶性糖和淀粉的含量有一定的差异,即叶部>茎部>根部;不同牧草间可溶性糖和 淀粉的含量也存在差异,即紫花苜蓿>胡枝子>沙打旺>柠条>羊柴.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.     

Tracking data management using topology of moving object database system for moving object constrained trajectory in LBS application

Advances in wireless technologies and positioning technologies and spread of wireless devices, an interest in LBS (Location Based Service) is arising. To provide location based service, tracking data should have been stored in moving object database management system (called MODBMS) with proper policies and managed efficiently. So the methods which acquire the location information at regular time intervals then, store and manage have been studied. In this paper, we suggest tracking data management techniques using topology that is corresponding to the moving path of moving object. In our techniques, we update the MODBMS when moving object arrived at a street intersection or a curved road which is represented as the node in topology and predict the location at past and future with attribute of topology and linear function. In this technique, location data that are corresponding to the node in topology are stored, thus reduce the number of update and amount of data. Also in case predicting the location,because topology are used as well as existing location information, accuracy for prediction is increased than applying linear function or spline function.