共查询到10条相似文献,搜索用时 62 毫秒
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Junhua Xiao Lanlan Yin Jianmin Li Hu Zu Zuomin Zhou Baige Zhao Jiahao Sha 《科学通报(英文版)》2002,47(11):896-901
Using cDNA microarray hybridization from a human testicular cDNA library, one gene exhibiting ten-fold difference at expression level between adult and embryo human testes was cloned and named NYD-SP9, which was believed to be involved in spermatogenesis. Southern blot hybridization results showed that NYD-SP9 expressed highly in testis but low in ovary. Protein motif analysis of this cDNA sequence revealed a cluster of phosphorylation sites, indicating its potential involvement in signal pathways during spermatogenesis. Furthermore, one transmembrane helix was predicted in N-terminal region, indicating that putative NYD-SP6 may be served as a transmembrane protein. The proximity of these potential phosphorylation sites to each other indicates that there may be interaction among these sites to regulate spermatogenesis. These findings suggested that protein kinase NYD-SP9 might play a role in male germ cell differentiation. 相似文献
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The dorso-ventral axis ofXenopus embryo is established after fertilization. Blastula stage blastomeres acquire different identities as they have inherited different maternal materials which distribute radial symmetrically along the animal vegetal aixs in the full-grown oocyte, and are rearranged by the cortical rotation triggered by fertilization. The vegetal blastmeres demonstrate the different dorsalization potencies in the previous transplantation experiments. The data of blastomere explanting and RT-PCR analyzing indicate that the dorsal ventral bias also exists among the animal blastomeres even during the early blastula stage. 相似文献
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The recombinant expression vector pGEMD-fhit which contains full encoding region offhit gene was constructed. The recombinant was introduced into the BL21 (DE3) strain ofE. coli and induced by 1 mmol/L IPTG to express a 29×103 polypeptide offhit fusion protein. And the 29×103 protein was sensitive and specific in reaction with anti-fhit antibody in Western blot.
Foundation item: Supported by the National Natural Science Foundation of China (39770373)
Biography: SUN Yan (1975−), female, Master of science, Research direction: gene engineering 相似文献
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目的: 研究豌豆根瘤菌 RL3841 的谷胱甘肽还原酶编码基因 gshR 的功能.方法: 采用基因同源重组构建了豌豆根瘤菌 gshR 基因突变株 RLgshR,探讨了基因突变对根瘤菌抗氧化和共生固氮的影响, 利用实时荧光定量 RT-PCR检测 gshR基因的 mRNA 表达水平.结果: gshR基因缺失不影响菌株在 AMS 基础培养基中的生长能力, 但突变株对氢过氧化枯( CuOOH) 和较高浓度的 H2O2氧化物敏感.结论: gshR 基因突变株 RLgshR 形成正常固氮根瘤, gshR 基因的表达不受 H2O2和共生环境诱导. 相似文献
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以大布苏盐碱湖分离菌Alkalibacterium sp. SL3的DNA为模板,利用PCR扩增α-半乳糖苷酶基因(galSL3),构建重组质粒pET-22b-galSL3,转化至大肠杆菌BL21(DE3)诱导表达重组酶(rGalSL3). 通过镍柱亲和层析分离纯化重组酶,并对纯化的重组酶进行性质研究. 研究表明,纯酶rGalSL3最适pH值为5.5,最适温度为55℃;该酶在pH值 5.0~10.0保持90%以上的剩余酶活,在50℃有非常好的热稳定性. 在0~1.5mol·L-1 NaCl溶液中该酶的酶活基本不受影响,在3.0mol·L-1 NaCl溶液中有很好的稳定性. Pb2+、Ca2+、Co2+、Li+、Na+、K+、Triton-100和β-mercaptoethanol等对重组酶的活性有明显的促进作用. 该酶水解对硝基苯基-α-D-吡喃葡萄糖苷的Km、vmax和kcat分别为(2.64±0.02)μmol·mL-1、(454.55±0.59)μmol·(mg·min)-1和(347.73±1.27)s-1. 重组酶可水解蜜二糖和棉籽糖,不能水解瓜尔豆胶. 相似文献
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MYB转录因子在调控植物生长发育和逆境响应方面发挥着重要的作用.通过克隆获得了核桃的1条R1-MYB类转录因子EFM基因(命名为JrEFM1),利用生物信息学和实时荧光定量RT-PCR(RT-qPCR)技术,分析在不同非生物及植物激素处理下JrEFM1的表达规律,探究了JrEFM1的基本生物学功能.结果显示,JrEFM1的编码区长为1 320 bp,编码蛋白含439个氨基酸,分子量为48.322 KDa,理论等电点为9.26.与葡萄、番薯等具有较近的进化关系.其启动子包含干旱胁迫(MBS)、热激响应(HSE)、水杨酸(SA)、玉米素(O2-site)和赤霉素响应(GARE-motif)等相关元件.对JrEFM1在干旱,冷害,热激,ABA,JA,SA处理下的表达情况进行分析,发现JrEFM1可被这些处理不同程度地诱导,同时根和叶表现出不同的转录水平.表明JrEFM1可响应逆境胁迫,并与激素信号通路相关;JrEFM1可作为核桃逆境响应机制研究及抗逆育种的优良候选基因. 相似文献
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本研究采集7个菲牛蛭种群共70份样品,利用基因组重测序和转录组测序数据,系统分析水蛭素基因在种内水平的变异特征。结果显示,所有水蛭素基因都存在丰富的种内变异,hirudin1和hirudin2变异率较高,hirudin3和hirudin5次之,hirudin4则相对保守。hirudin5存在S型和L型两种长度的转录本,分别具有4个和6个外显子。从选择压力指数(dN/dS)的中位数上看,hirudin2受到强烈正选择(dN/dS = 3.288),hirudin5受到微弱正选择(dN/dS = 1.110),而其余3个基因整体上受到纯化选择(dN/dS < 1)。5个水蛭素基因之间总体表达量差异极显著(P < 0.001),其中hirudin2的表达量最高,hirudin1次之,其余3个基因则明显偏低。hirudin2和hirudin5基因的表达量在7个种群间有极显著差异(P < 0.01),其余3个基因则未表现出种群之间的差异。本研究结果表明,菲牛蛭5个水蛭素在序列变异、选择压力和基因表达特征上都有巨大差异,为研究水蛭素的进化规律和药物开发提供丰富的遗传资源。 相似文献
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Plants are exposed to many potentially pathogenic microbes in the environment, but each species is only susceptible to a limited number of pathogens. The broad resistance is referred to as nonhost resistance. To date, little is known about the underlying mechanism of nonhost resistance and the signaling transduction process. Here we describe a simple method for isolating Arabidopsis nonhost resistance mutants against a nonadapted bacterial pathogen. A RAP2.6 promoter-driven LUC reporter system was developed to replace the tedious bacterial growth assay during the primary screening. The RAP2.6-LUC reporter gene is normally induced by the virulent bacterium Pseudomonas syringae pv tomato but not the nonadapted bacterium P. syringae pv phaseolicola. By using this method we iso- lated 4 mutants displaying strong reporter activity in response to P. syringae pv phaseolicola, which were characterized in some details, ebsl, ebs2, ebs3, and ebs4 (enhanced bacterial susceptibility) were compromised in resistance against P. syringae pv phaseolicola and/or P. syringae pv tomato. In addition, ebs4 showed enhanced hypersensitive response to the incompatible bacterium P. syringae pv tomato (avrB). These results demonstrated that the method is suited for large scale screening for nonhost resistance mutants. 相似文献