钝齿棒杆菌YL_1基因文库的建立及异亮氨酸基因在大肠杆菌中的克隆表达

以ｐＵＣ１９质粒为载体建立了异亮氨酸产生菌钝齿棒杆菌（Ｃｏｒｙｍｅｂａｃｔｅｒｉｕｍｃｒｅｎａｔｕｍ）ＹＬｌ的基因文库，并通过酶切重组子、斑点杂交得到证实．共筛选５８００个重组子，一个特定ＤＮＡ片断在库中存在的可靠性达９９．９９％．从中克隆重组了带有ｉｌｖＡ基因片断的重组质粒ｐＹＩ９４，转化ＡＨＶ的大肠杆菌ＥＤ８６５４－５，使本来不产Ｉｌｅ的大肠杆菌可以积累Ｉｌｅ１．５ｇ／Ｌ，为进一步高效表达构建工程菌创造了条件．     

重组质粒βhCG—pPIC9K的构建及嗜甲醇酵？…

目的：为用基因重组技术表达ｈＣＧ避孕疫苗抗原,构建在酵母细胞中表达的重组质粒βｈＣＧ－ｐＰＩＣ９Ｋ,转化嗜甲醇酵母,方法：根据βｈＣＧ的ｃＤＮＡ序列设计两条引物,使上游带ＥｃｏＲⅠ酶切位点,下游带ＮｏｔⅠ酶切位点,以质粒βｈＣＧ－ＰＢＳＫＳ为模板,进行ＰＣＲ扩增反应;将所得的ＤＮＡ片段经ＥｃｏＲⅠ和ＮｏｔⅠ双酶切后用Ｔ４连接酶与ｐＰＩＣ９Ｋ质粒进行连接,然后导入大肠杆菌ＤＨ５α,用ＰＣＲ筛选阳性克     

耐碱细菌NTT36耐碱基因的克隆及表达产物的分析

耐碱芽孢杆菌ＮＴＴ３６总ＤＮＡ经ＥｃｏＲ１酶不完全消化,与质粒ｐＵＣ１８的ＥｃｏＲＲＩ酶切产物在体外重组后,转化大肠杆菌ＨＢ１０１,在碱性平板上直接筛选耐碱转化子,转化经检测具有氨苄青霉素抗体,分析表明转化子具有１５ｋｂ大小的重组质粒,用此重组质粒转化大肠杆菌ＨＢ１０１,ＤＨ５ａ和ＸＬ１－Ｂｌｕｅ,均产生大量同时具有耐碱性和Ａｍｐ抗性的转化子,通过Ｓｏｕｔｈｅｒｎ杂交和点杂交证明此重组质粒（定名为     

重组人粒细胞集落刺激因子在Escherichia coli中的表达研究

根据人天然粒细胞集落刺激因子（Ｇ－ＣＳＦ）基因序列设计出引物,通过ＲＴ－ＰＣＲ从人外周血单个核细胞ｍＲＮＡ获得Ｇ－ＣＳＦｃＤＮＡ片段,将该片段与原核表达载体ｐＴ７构建成重组体,导入大肠杆菌后发现天然Ｇ－ＣＳＦｃＤＮＡ表达量并不理想,这可能是由于天然ｈＧ－ＣＳＦ５’端Ｇ＋Ｃ的比例过高,使转录后的ｍＲＡ很容易形成二级结构而影响翻译起始,因此在大肠杆菌中很难获得高效表达,根据密码子简并性原则,在不改变编码氨基酸顺序的前提下,通过重新设计ＰＣＲ引物,将ｈＧ－ＣＳＦ的前３个氨基酸密码子中的几个ＧＣ碱基作了改动,获得了新的ｃＤＮＡ突变体,将其与ＰＴ７的重组导入大肠杆菌,获得了高效表达。     

黄孢平革霉（Phanerochaete chrysosporium）木质素过氧化物酶基因的克隆与分析

用大肠杆菌克隆载体ｐＵＣ１０构建白腐丝状担子真菌黄孢平革霉（Ｐｈａｎｅｒｏｃｈａｅｔｅｃｈｒｙｓｏｓｐｏｒｉｕｍ）ＭＥ４４６基因组文库，用分离自ＢＫＭ－Ｆ１７６７菌株的木质素过氧化物酶ｃＤＮＡ片段ＣＬＧ４和ＣＬＧ５为探针，从构建的基因组文库中分离到一个含木质素过氧化物酶基因的重组子ｐＧＬＧ－Ｍ１。对该重组子插入片段所作的限制酶谱分析结果表明，它与来自ＢＫＭ－Ｆ１７６７的ＧＬＧ２片段的限制酶谱十分相似。     

环状芽胞杆菌（Bacillus circulans）基因表达效率调节DNA片段的研究

用大肠杆菌（Ｅｓｃｈｅｒｉｃｈｉａｃｏｌｉ）启动子探针型载体ｐＳＵＰＶ１从环状芽胞杆菌（Ｅａｃｉｌｌｕａｅｉｒｃｕｌａｎｓｃ－２）基因组中分离的启动子片段ＢＣ６能使无启动子的卡那霉素抗性基因恢复活性，并在大肠杆菌中获得高效表达（卡那霉素抗性高达１２００μｇ／ｍＬ）．当用限制酶ＸｂａⅠ去除其５′－端１．２ｋｂ片段（命名为Ｘｂ片段）后，３′－端片段仍能启动Ｋａｎ￣ｒ基因表达，其抗性水平降至４００μｇ／ｍＬ但当１．２ｋｂ片段反向插回原有位置时，其抗性水平降至６００μｇ／ｍＬ．将Ｘｂ片段正向插入另一重组质粒ｐＢＣ３中融合的Ｋａｎ￣ｒ基因启动子的上游时，卡那霉素抗性由２００μｇ／ｍＬ上升至１０００μｇ／ｍＬ．当Ｘｂ片段反向插入时，Ｋａｎ￣ｒ基因的表达却明显受到抑制，降为１００μｇ／ｍＬ．这说明Ｘｂ片段具有正向增强而反向衰减Ｋａｎ￣ｒ基因表达的能力．用ＢＣ６片段的两个亚克隆片段与ｃ－２菌株总ＤＮＡ分别作Ｓｏｕｔｈｅｒｎ杂交时，结果显示３′－端片段以单拷贝形式存在于基因组中，而Ｘｂ片段则以多拷贝形式散布其中．由此推断Ｘｂ片段并非一定伴随该基因启动子存在．     

豌豆cop1cDNA的克隆,序列分析及其在大肠杆菌中的表达

以拟南芥ｃｏｐ１ｃＤＮＡ为探针,从豌豆ＣＤＮＡ文库中克隆到了豌豆ｃｏｐ１ｃＤＮＡ。序列分析表明,它全长为２８６３ｂｐ,其中包括６０４ｂｐ５‘非编码区,２４３ｂｐ３’非编码区和２０１６ｂｐ编码区,编码６７２个氨基酸。在大肠杆菌中实现了豌豆ｃｏｐ１基因的高效表达。对拟南芥、豌豆和番茄３种植物ｃｏｐ１的序列同源性比较表明,ｃｏｐ１可能是一种进化上很保守的蛋白质。     

HVTG非必需基因区鉴定及含MDVgB基因重组病毒的构建

以火鸡疱疹病毒（ＨＶＴ）约８．０ｋｂ的ＢａｍＨＩ ＤＮＡ克隆片段中的ＢａｍＨＩ ＨｉｎｄⅢ亚克隆片段为同源序列,将大肠杆菌β－半乳糖苷酶（ＬａｃＺ）基因作为报告基因,通过同源重组,获得了表达ＬａｃＺ基因产物的重组ＨＶＴ（ＬａｃＺ－ｒＨＶＴ）。经本内,体外传代表明重组病毒中的ＬａｃＺ基因表达稳定,同时证明该亚克隆片段属于ＨＶＴ复制的非必需基因。在此基础上,将Ｉ型马立克氏病毒（ＭＤＶ）糖蛋白Ｂ（ｇＢ）     

重组人钙调素的表达及其McAb的制备与精子CaM定位

用基因重组技术构建人钙调素基因Ⅲ（ｈＣａＭⅢｃＤＮＡ）表达载体ｐＢＶ／ｈＣａＭⅢ,并在大肠杆菌ＤＨ５α中经热诱导获得可溶性ＣａＭ蛋白的高效表达．将纯化的重组ｈＣａＭ与异型双功能剂ＳＰＤＰ及鼠血清白蛋白（ＭＳＡ）交联,免疫Ｂａｌｂ／ｃ小鼠,用常规细胞融合技术制备单克隆抗体（ＭｃＡｂ）,得到３株稳定分泌单克隆抗体的杂交瘤细胞．间接ＥＬＩＳＡ和斑点免疫结果证实,三种单克隆抗体均与自制的ｒｈＣａＭ和ＣａＭ标准品起特异反应．用免疫组化法对精子中ＣａＭ定位,发现ＣａＭ主要分布于精子头颈部,不育组ＣａＭ＋精子率（（４５．０±７．５）％）显著低于生育组（（６８．５±１０．５）％）．     

YSM－1型清洁动物饮水消毒器的研制

我们研制成功一种经济实用、性能稳定、效果可靠、操作简便的饮水消毒器，采用耐酸碱抗腐蚀的高分子中空纤维非终断净化技术和紫外线３６０度全方位照射灭菌多级协同作用的原理。该消毒器如同一只手提箱大小，净重４ｋｇ左右。凡是有水源和２２０伏电源的任何场合都可使用，不受制约地随时接上电源打开水笼头即可提供（１５０－２００１／ｈ）洁净水，既可装瓶（２５０ｍｌ水瓶６００－８００瓶／小时）供动物饮用，又可作为清洁区卫生用水。根据需要可以加大流量，最大达３５０１／ｈ。生物效果的测试：在水压０．０５－０．０８ｐａ流量１５０－２００ｌ／ｈ的条件下，我们测试自来水中自然菌、人为加入大肠杆菌８０９９株、枯草芽胞杆菌ＡＴＣＣ９３７２株三种水样的消毒试验。并分别观察分级处理和全程协同处理的效果。全程协同效果：自来水中自然菌由５．２２ｃｆｎ／ｍｌ降到零（ｃｆｎ示细菌菌落）；大肠杆菌由５．６×１０￣６ｃｆｎ／ｍｌ降到零；枯草杆菌由５．０×１０￣６ｃｆｎ／ｍｌ降到零。仅经一二级处理大肠杆菌达９９．９９％的消除率，枯草杆菌也达９９．９％的消除率（因流量３００１／ｈ）；仅经三级处理大肠杆菌由５．６×１０５ｃｆｎ／ｍｌ降到零。     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

Perspherical Approaches for Electrons in Atoms and Charged Excitons in Quantum Wells

Instead of following Fock’s expansion,we solve the Schrodinger equation for some quantum mechanical manybody systems such as electrons in atoms and charged excitons in quantum wells in a similar way in hyperspherical coordinates by expanding the wave functions into orthonormal complete basis sets of the hyperspherical hannonics(HHs)of hyperangles and generalized Laguerre polynomials(GLPs)of the hyperradius.This leads the equation to     

Advances in broadband wireless communications under high-mobility scenarios

Future mobile communication systems aim at providing very high data transmission rates, even in high-mobility scenarios such as high-speed wheel-track trains, maglev trains, highway vehicles, airplanes, guided missiles or spacecraft. A particularly important commercial application is the strong and increasing worldwide demand for high- speed broadband wireless communications （up to 574.8 km/ h test speeds or 380 km/h commercial speeds） in railways, providing data, voice and video services for applications such as onboard entertainment services to passengers, train control, train dispatch, train sensor status handling and sur- veillance. In such high-mobility scenarios, there are a number of communication challenges, including fast hand- over, location updating, high-speed channel modeling, estimation and equalization, anti-Doppler spreading tech- niques, fast power control, and dedicated network architec- ture. Because signal transmission in very high-speed scenarios will inevitably experience serious deterioration, it is imperative to develop key broadband mobile communi- cation techniques for such high-speed vehicles.     

Wuhan University Journal of Natural Sciences TOTAL CONTENTS

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海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Scale-based spatial data model for GIS

Being the primary media of geographical information and the elementary objects manipulated, almost all of maps adopt the layer-based model to represent geographic information in the existent GIS. However, it is difficult to extend the map represented in layer-based model. Furthermore, in Web-Based GIS, It is slow to transmit the spatial data for map viewing. In this paper, for solving the questions above, we have proposed a new method for representing the spatial data. That is scale-based model. In this model we represent maps in three levels: scale-view, block, and spatial object, and organize the maps in a set of map layers, named Scale-View, which associates some given scales.Lastly, a prototype Web-Based GIS using the proposed spatial data representation is described briefly.     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to