魔芋不同外植体诱导比较实验

通过组织培养的方法 ,以魔芋顶芽、顶芽鳞片、侧芽、皮上芽苞、拟块茎鳞片为外植体 ,在各自最适培养基上进行愈伤组织诱导 ,拟获得了魔芋诱导的最好外植体材料 .结果表明 :拟块茎鳞片形成愈伤组织平均数为 5 4 ,而侧芽形成愈伤组织平均数只有 2 6 .形成的愈伤组织在 MS 6 - BA2 .0 mg/ L NAA0 .5 mg/ L 蔗糖 30 g/ L 培养基上进行不定芽诱导和扩繁 ,魔芋拟块茎鳞片不定芽的诱导率为 96 .4 % ,繁殖系数为 4 .9;而其它外植体最高的不定芽的诱导率为 74 .2 % ,最高的繁殖系数为 4 .1.将形成的幼苗生根 ,在精心管理下 ,试管苗移栽的成活率可达10 0 %     

观赏凤梨组织培养不同外植体的比较试验

选用观赏凤梨不同品种的老叶鞘、嫩叶鞘、顶芽、侧芽、茎段、叶片等不同组织作外植体 ,接种在诱导培养基为改良 MS+ 2 .0 mg· L- 1的 BA+ 5 .0 mg· L- 1的 2 ,4- D+ 0 .2 mg· L- 1的 IBA,及分化培养基为改良 MS+ 4 .0 mg· L- 1的 BA+ 0 .2 mg·L- 1的 IBA,培养 60 d进行统计 ,老叶鞘的愈伤组织三个品种平均诱导率为 74.6% ,丛生芽的诱导率平均数达 1 7.1 % ,次之是侧芽、顶芽 ,嫩叶鞘、茎段、叶片 ,后者能诱导出愈伤组织 ,不能诱导出丛生芽     

中国水仙愈伤组织诱导及植株再生研究

以中国水仙鳞茎为试材,进行中国水仙愈伤组织诱导及其分化的研究.结果表明:1)2%次氯酸钠20 min较适合水仙花花苞的消毒处理,水仙鳞茎的消毒处理以0. 1%升汞12 min灭菌效果较好,以内层鳞片存活率最高,外层鳞片次之;2)在MS+6-BA2. 0 mg·L-1+2,4-D 0. 5 mg·L-1为愈伤诱导及继代的培养基上,子房壁愈伤诱导率最高,为77. 8%,但继代未见再分化,外层鳞片愈伤诱导率次之,外层鳞片与带鳞片的鳞茎盘诱导的愈伤均能继代发育成小鳞茎;3)以培养基MS+6-BA 2. 0 mg·L-1+NAA 0. 5 mg·L-1较适合外层鳞片愈伤组织的再分化,鳞茎分化率达64. 4%;4)在培养基1/2 MS+IBA1. 0 mg·L-1上,水仙鳞茎苗生根效果较好,生根率达86. 7%,移栽成活率高.     

火炬姜叶片培养及植株再生

报道了火炬姜叶片离体培养的研究结果 ,试验表明 :诱导叶片产生愈伤组织效果较好的激素配比是 6 -BA 0 .5mg·L- 1+ 2 ,4 -D 0 .5mg·L- 1;最适于愈伤组织分化的基本培养基是MS ;6 -BA、ZT和KT均能诱导愈伤组织分化不定芽 ,其中以 6 -BA的效果较好和经济 ;在所试的 6 -BA质量浓度中 ,以 3mg·L- 1的效果较佳 ;添加 80 0mg·L- 1的CH有利于火炬姜愈伤组织的分化 ;生根培养阶段 ,以附加w =0 .15 %的活性炭的 1/ 2MS培养基效果较好 ,生根率达 10 0 % ,且植株生长健壮 ;试管苗移栽在表土上 ,成活率可达 10 0 %     

玉米幼叶愈伤组织诱导和不定根的再生

以 7d龄左右的玉米 (ZeamaysL .)实生苗幼叶不同切段为材料 ,在添加不同植物激素的 1/ 2MS培养基上培养 ,2周后可以诱导出愈伤组织 ,第 2叶基部愈伤组织诱导率高于第 1叶 .MS预处理培养基中添加 1mg·L- 1呋喃腺嘌呤 (KT)或 1mg·L- 1苄基腺嘌呤 (BA)后 ,使诱导频率提高到 5 8.3%,并不同程度地诱导不定根的发生 ;诱导培养基中添加 1mg·L- 1KT或 1mg·L- 1BA对愈伤组织和不定根的诱导均有抑制作用 .当在诱导培养基中添加 5 0 0mg·L- 1水解酪蛋白(CH)时 ,诱导培养基中BA或KT对愈伤组织和不定根发生的抑制作用得到缓解 .     

降低何首乌愈伤组织褐化研究

以何首乌为材料, 叶片愈伤组织为外植体, 采用L9 (34)正交设计, 研究不同因素对降低愈伤组织褐化的作用. 3因素为基本培养基(A)、培养基中活性炭(B)和硝酸银(C)浓度, 每因素设3个水平, A分别为MS, 1/2 MS和1/4 MS无机盐浓度, B分别为0 g·L-1, 5 g·L-1和10 g·L-1, C分别为5 mg·L-1, 0 mg·L-1和10 mg·L-1.外植体接种在不同的MS基本培养基中(含2,4 D 1 mg·L-1、琼脂8 g·L-1、蔗糖25 g·L-1, pH=5 8), 于(25±1) ℃、2 000 lx光照培养, 30 d后统计愈伤组织的褐化率. 试验结果表明: 降低愈伤组织褐化的最优水平组合为A2B3C1, B对试验结果的影响达显著水平(p<0 05). 以1/2 MS为基本培养基, 并于1/2 MS+活性炭10 g·L-1+AgNO3 5 mg·L-1培养基(附含2,4 D 1 mg·L-1)中培养, 褐化率可降至13 9%.     

植物激素对一品红愈伤组织和胚状体的作用

以一品红幼茎为外植体,接种在培养基(MS+ZT)0.5mg·L-1、NAA0.5mg·L-1、IBA0.5mg·L-1、2,4 D1.0mg·L-1、6 BA1.0mg·L-1诱导愈伤组织,其中以NAA0.5mg·L-1时愈伤组织诱导率最高,为96.4%.将继代培养的愈伤组织,接种到(MS+ZT)1.0mg·L-1+NAA0～2.0mg·L-1、IBA0～2.0mg·L-1、2,4 D0～2.0mg·L-1;(MS+6 BA)1.0mg·L-1+NAA0～2.0mg·L-1、2,4 D0 2.0mg·L-1培养基上,进行胚状体的分化培养,其中以ZT1.0mg·L-1+2,4 D0.5mg·L-1效果最好.     

甘蔗组织培养中培养基的优化

比较了不同基本培养基 ,不同激素及其不同质量浓度的组合对甘蔗愈伤组织的诱导 ,不定芽的分化及其生根的效果 .结果表明 :MS + 2 ,4 -D 1～ 5mg·L-1培养基均可诱导愈伤组织的增殖 ;MS +KT 1.5～ 2 .0mg·L-1+ 6 -BA 2mg·L-1培养基对愈伤组织分化不定芽的效果最佳 ;1 2MS +NAA 1～ 4mg·L-1培养基对生根较好 .     

白魔芋外植体组培分化条件的研究

用白魔芋的5种营养器官作外植体在11种培养基上进行组织培养,M6培养基适于侧芽生长和皮下组织诱导愈伤,M9培养基适于主芽生长和基部组织诱导愈伤,M2培养基适于皮上式苞组织分化生长,且皮上芽苞组织为最好的接种材料。     

曼地亚红豆杉愈伤组织诱导研究

为研究曼地亚红豆杉愈伤组织的诱导最佳条件,以新生嫩茎为外植体筛选最佳的灭菌方案,以新生的幼茎和叶片为外植体筛选出诱导愈伤组织适宜的外植体和最佳的激素配比。结果表明：75%酒精处理30s＋0.1%HgC12处理12min灭菌效果最好;幼茎比叶片更适合愈伤组织诱导,在MS基础上,最佳的幼茎愈伤组织诱导激素配比为0.3mg.L-16-BA、1.0mg.L-1NAA、3.0mg.L-12,4-D,此时诱导率达到93.3%,愈伤组织生长质量好。     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

Exciton Seebeck in Molecular Systems

Various applications relevant to the exciton dynamics,such as the organic solar cell,the large-area organic light-emitting diodes and the thermoelectricity,are operating under temperature gradient.The potential abnormal behavior of the exicton dynamics driven by the temperature difference may affect the efficiency and performance of the corresponding devices.In the above situations,the exciton dynamics under temperature difference is mixed with     

Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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An on-line scaling method for improving scalability of a database cluster

The explosive growth of the Internet and database applications has driven database to be more scalable and available, and able to support on-line scaling without interrupting service. To support more client's queries without downtime and degrading the response time, more nodes have to be scaled up while the database is running. This paper presents the overview of scalable and available database that satisfies the above characteristics. And we propose a novel on-line scaling method. Our method improves the existing on-line scaling method for fast response time and higher throughputs. Our proposed method reduces unnecessary network use, i.e. , we decrease the number of data copy by reusing the backup data. Also, our on-line scaling operation can be processed parallel by selecting adequate nodes as new node. Our performance study shows that our method results in significant reduction in data copy time.     

An improved R-tree based on childnode''s probability

R-Tree is a good structure for spatial searching. But in this indexing structure,either the sequence of nodes in the same level or sequence of traveling these nodes when queries are made is random. Since the possibility that the object appears in different MBR which have the same parents node is different, if we make the subnode who has the most possibility be traveled first, the time cost will be decreased in most of the cases. In some case, the possibility of a point belong to a rectangle will shows direct proportion with the size of the rectangle. But this conclusion is based on an assumption that the objects are symmetrically distributing in the area and this assumption is not always coming into existence. Now we found a more direct parameter to scale the possibility and made a little change on the structure of R-tree, to increase the possibility of founding the satisfying answer in the front sub trees. We names this structure probability based arranged R-tree (PBAR-tree).     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

Integrating GIS Web services based on mediating architecture

The geographic information service is enabled by the advancements in general Web service technology and the focused efforts of the OGC in defining XML-based Web GIS service. Based on these models, this paper addresses the issue of services chaining,the process of combining or pipelining results from several interoperable GIS Web Services to create a customized solution. This paper presents a mediated chaining architecture in which a specific service takes responsibility for performing the process that describes a service chain. We designed the Spatial Information Process Language (SIPL) for dynamic modeling and describing the service chain, also a prototype of the Spatial Information Process Execution Engine (SIPEE) is implemented for executing processes written in SIPL. Discussion of measures to improve the functionality and performance of such system will be included.