共查询到20条相似文献,搜索用时 31 毫秒
1.
Karina R. Bortoluci Ruslan Medzhitov 《Cellular and molecular life sciences : CMLS》2010,67(10):1643-1651
Cells can die by distinct mechanisms with particular impacts on the immune response. In addition to apoptosis and necrosis, recent studies lead to characterization of a new pro-inflammatory form of cell death, pyroptosis. TLR and NLR, central innate immune sensors, can control infections by modulating host cell survival. In addition, TLRs can promote the induction of autophagy, thus promoting delivery of infecting pathogens to the lysosomes. On the other hand, activation of some NLR members, especially NLRC4 and NAIP5, leads to the infected cell death by pyroptosis, which is accompanied by secretion of the pro-inflammatory cytokines IL-1β, IL-18, and IL-33. Data presented here illustrate how the compartmentalization of the innate immune sensors can influence the outcome of infections by controlling the fate of host cells. 相似文献
2.
Makeda Robinson Stanford Schor Rina Barouch-Bentov Shirit Einav 《Cellular and molecular life sciences : CMLS》2018,75(20):3693-3714
Viruses are obligate intracellular pathogens that are dependent on cellular machineries for their replication. Recent technological breakthroughs have facilitated reliable identification of host factors required for viral infections and better characterization of the virus–host interplay. While these studies have revealed cellular machineries that are uniquely required by individual viruses, accumulating data also indicate the presence of broadly required mechanisms. Among these overlapping cellular functions are components of intracellular membrane trafficking pathways. Here, we review recent discoveries focused on how viruses exploit intracellular membrane trafficking pathways to promote various stages of their life cycle, with an emphasis on cellular factors that are usurped by a broad range of viruses. We describe broadly required components of the endocytic and secretory pathways, the Endosomal Sorting Complexes Required for Transport pathway, and the autophagy pathway. Identification of such overlapping host functions offers new opportunities to develop broad-spectrum host-targeted antiviral strategies. 相似文献
3.
Dawn M. Walker Steve Oghumu Gaurav Gupta Bradford S. McGwire Mark E. Drew Abhay R. Satoskar 《Cellular and molecular life sciences : CMLS》2014,71(7):1245-1263
Numerous disease-causing parasites must invade host cells in order to prosper. Collectively, such pathogens are responsible for a staggering amount of human sickness and death throughout the world. Leishmaniasis, Chagas disease, toxoplasmosis, and malaria are neglected diseases and therefore are linked to socio-economical and geographical factors, affecting well-over half the world’s population. Such obligate intracellular parasites have co-evolved with humans to establish a complexity of specific molecular parasite–host cell interactions, forming the basis of the parasite’s cellular tropism. They make use of such interactions to invade host cells as a means to migrate through various tissues, to evade the host immune system, and to undergo intracellular replication. These cellular migration and invasion events are absolutely essential for the completion of the lifecycles of these parasites and lead to their for disease pathogenesis. This review is an overview of the molecular mechanisms of protozoan parasite invasion of host cells and discussion of therapeutic strategies, which could be developed by targeting these invasion pathways. Specifically, we focus on four species of protozoan parasites Leishmania, Trypanosoma cruzi, Plasmodium, and Toxoplasma, which are responsible for significant morbidity and mortality. 相似文献
4.
Signalling in viral entry 总被引:9,自引:0,他引:9
Greber UF 《Cellular and molecular life sciences : CMLS》2002,59(4):608-626
Viral infections are serious battles between pathogens and hosts. They can result in cell death, elimination of the virus
or latent infection keeping both cells and pathogens alive. The outcome of an infection is often determined by cell signalling.
Viruses deliver genomes and proteins with signalling potential into target cells and thereby alter the metabolism of the host.
Virus interactions with cell surface receptors can elicit two types of signals, conformational changes of viral particles,
and intracellular signals triggering specific cellular reactions. Responses by cells include stimulation of innate and adaptive
immunity, growth, proliferation, survival and apoptosis. In addition, virus-activated cell signalling boosts viral entry and
gene delivery, as recently shown for adenoviruses and adeno-associated viruses. This review illustrates that multiple activation
of host cells during viral entry profoundly impacts the elaborate relationship between hosts and viral pathogens.
Received 13 September 2001; received after revision 23 October 2001; accepted 16 November 2001 相似文献
5.
Michael R. Yeaman 《Cellular and molecular life sciences : CMLS》2010,67(4):525-544
Platelets interact with bacterial pathogens through a wide array of cellular and molecular mechanisms. The consequences of
this interaction may significantly influence the balance between infection and immunity. On the one hand, recent data indicate
that certain bacteria may be capable of exploiting these interactions to gain a virulence advantage. Indeed, certain bacterial
pathogens appear to have evolved specific ways in which to subvert activated platelets. Hence, it is conceivable that some
bacterial pathogens exploit platelet responses. On the other hand, platelets are now known to possess unambiguous structures
and functions of host defense effector cells. Recent discoveries emphasize critical features enabling such functions, including
expression of toll-like receptors that detect hallmark signals of bacterial infection, an array of microbicidal peptides,
as well as other host defense molecules and functions. These concepts are consistent with increased risk and severity of bacterial
infection as correlates of clinical abnormalities in platelet quantity and quality. In these respects, the molecular and cellular
roles of platelets in host defense against bacterial pathogens are explored with attention on advances in platelet immunobiology. 相似文献
6.
Stephanie Gras Ildiko Van Rhijn Adam Shahine Jérôme Le Nours 《Cellular and molecular life sciences : CMLS》2018,75(9):1623-1639
The immune system has evolved to protect hosts from pathogens. T cells represent a critical component of the immune system by their engagement in host defence mechanisms against microbial infections. Our knowledge of the molecular recognition by T cells of pathogen-derived peptidic antigens that are presented by the major histocompatibility complex glycoproteins is now well established. However, lipids represent an additional, distinct chemical class of molecules that when presented by the family of CD1 antigen-presenting molecules can serve as antigens, and be recognized by specialized subsets of T cells leading to antigen-specific activation. Over the past decades, numerous CD1-presented self- and bacterial lipid-based antigens have been isolated and characterized. However, our understanding at the molecular level of T cell immunity to CD1 molecules presenting microbial lipid-based antigens is still largely unexplored. Here, we review the insights and the molecular basis underpinning the recognition of microbial lipid-based antigens by T cells. 相似文献
7.
Janesh Pillay Tamar Tak Vera M. Kamp Leo Koenderman 《Cellular and molecular life sciences : CMLS》2013,70(20):3813-3827
Neutrophils are essential effector cells in the host defense against invading pathogens. Recently, novel neutrophil functions have emerged in addition to their classical anti-microbial role. One of these functions is the suppression of T cell responses. In this respect, neutrophils share similarities with granulocytic myeloid-derived suppressor cells (G-MDSCs). In this review, we will discuss the similarities and differences between neutrophils and G-MDSCs. Various types of G-MDSCs have been described, ranging from immature to mature cells shaping the immune response by different immune suppressive mechanisms. However, all types of G-MDSCs share distinct features of neutrophils, such as surface markers and morphology. We propose that G-MDSCs are heterogeneous and represent novel phenotypes of neutrophils, capable of suppressing the immune response. In this review, we will attempt to clarify the differences and similarities between neutrophils and G-MDSCs and attempt to facilitate further research. 相似文献
8.
One of the most important opportunistic pathogens associated with acquired immunodeficiency syndrome (AIDS) is the M. avium complex. M. avium infections are found in up to 70% of individuals in advanced stages of AIDS. It is apparent that M. avium can replicate in host macrophages and persist for long periods. This group of mycobacteria are distinguished by the presence
of unique, highly antigenic, surface-located lipids known as the glycopeptidolipids (GPLs). The GPLs are the chemical basis
of the 31 distinct serovars of the M. avium complex, and have also been identified in some other species. The M. avium lipids are immunosuppressive and can induce a variety of cytokines that affect general host responses. Despite extensive
chemical characterization of the structures of these GPLs, much work is needed to elucidate the molecular mechanism involved
in this complex glycosylation pathway and its genetic basis. The challenges for the future lie in explaining the roles of
these copious products in the intracellular life and infectivity of mycobacteria. The intention of our review is to offer
a concise account of the structures of the M. avium lipids, their putative roles in the host responses, bacterial physiology and pathogenesis, particularly in immunocompromised
patients such as those infected with human immunodeficiency virus (HIV). Advances in chemical synthesis of the various haptenic
oligosaccharides are also given to demonstrate how these have helped to define the immunogenic determinants. We believe that
future research should involve the creation of conditional mutants defective in these lipids for both functional and biosynthesis
studies which will complement biological assays using chemically defined or modified neoglycoconjugates.
Received 7 May 2001; received after revision 28 June 2001; accepted 28 June 2001 相似文献
9.
B cells express immunoglobulins on their surface where they serve as antigen receptors. When secreted as antibodies, the same
molecules are key elements of the humoral immune response against pathogens such as viruses. Although most antibodies are
restricted to binding a specific antigen, some are polyreactive and have the ability to bind to several different ligands,
usually with low affinity. Highly polyreactive antibodies are removed from the repertoire during B-cell development by physiologic
tolerance mechanisms including deletion and receptor editing. However, a low level of antibody polyreactivity is tolerated
and can confer additional binding properties to pathogen-specific antibodies. For example, high-affinity human antibodies
to HIV are frequently polyreactive. Here we review the evidence suggesting that in the case of some pathogens like HIV, polyreactivity
may confer a selective advantage to pathogen-specific antibodies. 相似文献
10.
T. R. Garbe 《Cellular and molecular life sciences : CMLS》1992,48(7):635-639
Invasive microorganisms encounter defensive attempts of the host to starve, destroy and eliminate the infection. In experimental model systems aiming to imitate defensive actions of the host, microorganisms respond by the rapid acceleration in the rate of expression of heat shock and other stress proteins. Heat shock proteins (hsp) of most if not all pathogens are major immune targets for both B- and T-cells. Host cells involved in the defensive action cannot avoid exposure to their own reactive compounds, such as oxygen radicals, resulting in premature cell death and tissue damage. Long-term consequences to the host may include cancer. In cells in tissue culture, induction of host-specific hsps occurs upon exposure to oxidants and in viral infections. Drugs that bind to members of the hsp70 family induce peroxisome proliferation and hepatocarcinoma, but may open the way for the development of novel drugs in support of antimetabolite treatment of infections and cancer. 相似文献
11.
T R Garbe 《Experientia》1992,48(7):635-639
Invasive microorganisms encounter defensive attempts of the host to starve, destroy and eliminate the infection. In experimental model systems aiming to imitate defensive actions of the host, microorganisms respond by the rapid acceleration in the rate of expression of heat shock and other stress proteins. Heat shock proteins (hsp) of most if not all pathogens are major immune targets for both B- and T-cells. Host cells involved in the defensive action cannot avoid exposure to their own reactive compounds, such as oxygen radicals, resulting in premature cell death and tissue damage. Long-term consequences to the host may include cancer. In cells in tissue culture, induction of host-specific hsps occurs upon exposure to oxidants and in viral infections. Drugs that bind to members of the hsp70 family induce peroxisome proliferation and hepatocarcinoma, but may open the way for the development of novel drugs in support of antimetabolite treatment of infections and cancer. 相似文献
12.
The macromolecular peptide-loading complex in MHC class I-dependent antigen presentation 总被引:5,自引:1,他引:4
A challenging task for the adaptive immune system of vertebrates is to identify and eliminate intracellular antigens. Therefore
a highly specialized antigen presentation machinery has evolved to display fragments of newly synthesized proteins to effector
cells of the immune system at the cell surface. After proteasomal degradation of unwanted proteins or defective ribosome products,
resulting peptides are translocated into the endoplasmic reticulum by the transporter associated with antigen processing and
loaded onto major histocompatibility complex (MHC) class I molecules. Peptide-MHC I complexes are transported via the secretory
pathway to the cell surface where they are then inspected by cytotoxic T lymphocytes, which can trigger an immune response.
This review summarizes the current view of the intracellular machinery of antigen processing and of viral immune escape mechanisms
to circumvent destruction by the host.
Received 4 October 2005; received after revision 19 November 2005; accepted 24 November 2005 相似文献
13.
Cationic host defence peptides: Innate immune regulatory peptides as a novel approach for treating infections 总被引:9,自引:0,他引:9
An increase in antibiotic resistance and the emergence of new pathogens has led to an urgent need for alternative approaches
to infection management. Immunomodulatory molecules that do not target the pathogen directly, but rather selectively enhance
and/or alter host defence mechanisms, are attractive candidates for therapeutic development. Natural cationic host defence
peptides represent lead molecules that boost innate immune responses and selectively modulate pathogen-induced inflammatory
responses. This review discusses recent evidence exploring the mechanisms of cationic host defence peptides as innate immune
regulators, their role in the interface of innate and adaptive immunity, and their potential application as beneficial therapeutics
in overcoming infectious diseases.
Received 3 November 2006; received after revision 14 December 2006; accepted 22 January 2007 相似文献
14.
Toxoplasma gondii is an obligate intracellular parasite that can infect virtually any nucleated cell. During invasion Toxoplasma creates the parasitophorous vacuole, a subcellular compartment that acts as an interface between the parasite and host, and serves as a platform for modulation of host cell functions that support parasite replication and infection. Spatial reorganization of host organelles and cytoskeleton around the parasitophorous vacuole are observed following entry, and recent evidence suggests this interior redecorating promotes parasite nutrient acquisition. New findings also reveal that Toxoplasma manipulates host signaling pathways by deploying parasite kinases and a phosphatase, including at least two that infiltrate the host nucleus. Toxoplasma infection additionally controls several cellular pathways to establish an anti-apoptotic environment, and subverts immune cells as a conduit for dissemination. In this review we discuss these recent developments in understanding how Toxoplasma achieves widespread success as a human and animal parasite by manipulating its host. 相似文献
15.
Following infection, a virus must battle against the host's immune response. Viruses have developed many ways to escape immune surveillance and downregulate the host's immune response. Some viruses cause a generalized immunosuppression, thereby inhibiting or depressing the immune response towards themselves as well as towards unrelated pathogens. This review will focus on the mechanisms involved in the three main human viral infections causing immunosuppression: measles, human immunodeficiency virus and cytomegalovirus. We will also discuss what has been learned from the extensively studied mouse models of viral-induced immunosuppression: lymphocytic choriomeningitis virus and Rauscher leukemia virus. All of these viruses that induce generalized immunosuppression appear to do so by very similar mechanisms. They hinder antigen presentation to T cells and/or hematopoiesis. We will highlight the similarities in the viral targets as well as present evidence for alternate mechanisms. 相似文献
16.
In Young Hwang Hui Ling Lee James Guoxian Huang Yvonne Yijuan Lim Wen Shan Yew Yung Seng Lee Matthew Wook Chang 《Cellular and molecular life sciences : CMLS》2018,75(15):2719-2733
Lack of pathogen specificity in antimicrobial therapy causes non-discriminant microbial cell killing that disrupts the microflora present. As a result, potentially helpful microbial cells are killed along with the pathogen, altering the biodiversity and dynamic interactions within the population. Moreover, the unwarranted exposure of antibiotics to microbes increases the likelihood of developing resistance and perpetuates the emergence of multidrug resistance. Synthetic biology offers an alternative solution where specificity can be conferred to reduce the non-specific, non-targeted activity of currently available antibiotics, and instead provides targeted therapy against specific pathogens and minimising collateral damage to the host’s inherent microbiota. With a greater understanding of the microbiome and the available genetic engineering tools for microbial cells, it is possible to devise antimicrobial strategies for novel antimicrobial therapy that are able to precisely and selectively remove infectious pathogens. Herein, we review the strategies developed by unlocking some of the natural mechanisms used by the microbes and how these may be utilised in targeted antimicrobial therapy, with the promise of reducing the current global bane of multidrug antimicrobial resistance. 相似文献
17.
Host recognition by toxigenic plant pathogens 总被引:5,自引:0,他引:5
Certain fungal pathogens release host-selective (or host-specific) toxins (HST) as a host recognition factor during spore germination at the infection site on plants. Prior to penetration of the pathogen into its host, the released toxin specifically binds to a putative receptor on the host cells and initiates signaling mechanisms leading to pleiotropic effects on cells. Of these, the crucial one negates the general and inducible defense reactions of the cells. This is accomplished by a signal from the HST, which is transduced through a path way at or near the step of plasma membrane modulation, which is directly or indirectly triggered by the HST. This mechanism operates even though the toxin may affect mitochondria or chloroplasts as the primary target organelle. The fungal spore is able to penetrate the so-called 'narcotized cell' and completes the initial colonization of the host. The host recognition process may take place without necessitating host cell death, even in the case of perthophytic parasites. At the molecular level, HST-mediated recognition of the host by a pathogen requires strict stereochemical precision like a lock and key. 相似文献
18.
The facultative intracellular pathogen Salmonella enterica resides in a special membrane compartment of the host cell and modifies its host to achieve intracellular survival and proliferation. The type III secretion system encoded by Salmonella pathogenicity island 2 (SPI2) has a central role in the interference of intracellular Salmonella with host cell functions. SPI2 function affects antimicrobial defense mechanisms of the host, intracellular transport processes, integrity and function of the cytoskeleton and host cell death. These modifications are mediated by translocation of a large number of effector proteins by the SPI2 system. In this review, we summarize recent work on the cellular phenotypes related to SPI2 function and contribution of SPI2 effector proteins to these manipulations. These studies reveal a complex set of pathogenic interferences between intracellular Salmonella and its host cells.Received 11 June 2004; received after revision 8 July 2004; accepted 12 July 2004 相似文献
19.
The human leukemia cell line, THP-1: a multifacetted model for the study of monocyte-macrophage differentiation 总被引:8,自引:0,他引:8
J Auwerx 《Experientia》1991,47(1):22-31
THP-1 is a human monocytic leukemia cell line. After treatment with phorbol esters, THP-1 cells differentiate into macrophage-like cells which mimic native monocyte-derived macrophages in several respects. Compared to other human myeloid cell lines, such as HL-60, U937, KG-1, or HEL cell lines, differentiated THP-1 cells behave more like native monocyte-derived macrophages. Because of these characteristics, the THP-1 cell line provides a valuable model for studying the mechanisms involved in macrophage differentiation, and for exploring the regulation of macrophage-specific genes as they relate to physiological functions displayed by these cells. 相似文献
20.
In the last decade intensive research has been conducted to determine the role of innate immunity host defense peptides (also termed antimicrobial peptides) in the killing of prokaryotic and eukaryotic cells. Many antimicrobial peptides damage the cellular membrane as part of their killing mechanism. However, it is not clear what makes cancer cells more susceptible to some of these peptides, and what the molecular mechanisms underlying these activities are. Two general mechanisms were suggested: (i) plasma membrane disruption via micellization or pore formation, and (ii) induction of apoptosis via mitochondrial membrane disruption. To be clinically used, these peptides need to combine high and specific anticancer activity with stability in serum. Although so far very limited, new studies have paved the way for promising anticancer host defense peptides with a new mode of action and with a broad spectrum of anticancer activity. 相似文献