共查询到20条相似文献,搜索用时 31 毫秒
1.
Wang SH Shih YL Ko WC Wei YH Shih CM 《Cellular and molecular life sciences : CMLS》2008,65(22):3640-3652
The cytotoxicity of cadmium (Cd) induced autophagy and apoptosis in MES-13 cells was determined by flow cytometry. Autophagy
was also assessed by formation of autophagosomes and processing of LC3. Pharmacological inhibition of autophagy resulted in
increased of cell viability, suggesting autophagy plays a role in cell death in Cd-treated mesangial cells. Cd also induced
a rapid elevation in cytosolic calcium ([Ca2+]i ), and modulation of [Ca2+]i via treatment with IP
3R inhibitor or knockdown of calcineurin resulted in a change in the proportion of cell death, suggesting that the release
of calcium from the ER plays a crucial role in Cd-induced cell death. Inhibition of Cd-induced ERK activation by PD 98059
suppressed Cd-induced autophagy, and BAPTA-AM eliminated activation of ERK. BAPTA-AM also inhibited Cd-induced mitochondrial
depolarization and activation of caspases. These findings demonstrated that Cd induces both autophagy and apoptosis through
elevation of [Ca2+]i, followed by Ca2+-ERK and Ca2+-mitochondria-caspase signaling pathways.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Received 05 July 2008; received after revision 25 August 2008; accepted 17 September 2008 相似文献
2.
B. C. Yoo S-H. Hong J-L. Ku Y-H. Kim Y-K. Shin S-G. Jang I-J. Kim S-Y. Jeong J-G. Park 《Cellular and molecular life sciences : CMLS》2009,66(2):350-364
Comparative analysis of proteomes using 5-fluorouracil (5-FU)-resistant human colon cancer cell line revealed that decreased
galectin-3 expression was significantly associated with retarded proliferation. However, in the presence of 5-FU proliferation
rate of cells with suppressed galectin-3 expression did not differ from that of cells with normal galectin-3 expression, even
galectin-3 suppression augmented apoptosis. Mechanism by which galectin-3 regulates cancer cell proliferation has been identified
in immunoprecipitates of the anti-galectin-3 antibody. Heterogeneous nuclear ribonucleoprotein Q (hnRNP Q) was identified
as a protein interacting with galectin-3. Interestingly, while galectin-3 protein was not affected by the hnRNP Q level, its
suppression was accompanied by a decrease in hnRNP Q expression. The present study demonstrates that galectin-3 stabilizes
hnRNP Q via complex formation, and reduction in the hnRNP Q level leads to slow proliferation and less susceptibility to 5-FU.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
B.C.Yoo; S-H.Hong; These two authors contributed equally to this work.
Received 10 September 2008; received after revision 19 October 2008; accepted 07 November 2008 相似文献
3.
The mitogen-activated protein kinase (MAPK) pathways are known to be involved in various processes of growth, differentiation
and cell death. In spite of their ubiquitous presence and seemingly enormous cross-talk with each other, their action is very
specific. This review deals with various aspects of the three different MAPK pathways (ERK, p38 and JNK) and how their specificity
is brought about.
Received 1 April 2008; received after revision 18 June 2008; accepted 18 June 2008 相似文献
4.
5.
Mitochondrial dysfunction and protein kinase C (PKC) activation are consistently found in diabetic cardiomyopathy but their
relationship remains unclear. This study identified mitochondrial aconitase as a downstream target of PKC activation using
immunoblotting and mass spectrometry, and then characterized phosphorylation-induced changes in its activity in hearts from
type 1 diabetic rats. PKCβ2 co-immunoprecipitated with phosphorylated aconitase from mitochondria isolated from diabetic hearts. Augmented phosphorylation
of mitochondrial aconitase in diabetic hearts was found to be associated with an increase in its reverse activity (isocitrate
to aconitate), while the rate of the forward activity was unchanged. Similar results were obtained on phosphorylation of mitochondrial
aconitase by PKCβ2 in vitro. These results demonstrate the regulation of mitochondrial aconitase activity by PKC-dependent phosphorylation. This may
influence the activity of the tricarboxylic acid cycle, and contribute to impaired mitochondrial function and energy metabolism
in diabetic hearts.
Received 31 October 2008; received after revision 17 December 2008; accepted 2 January 2009 相似文献
6.
C.-J. Chang P.-H. Yin D.-M. Yang C.-H. Wang W.-Y. Hung C.-W. Chi Y.-H. Wei H.-C. Lee 《Cellular and molecular life sciences : CMLS》2009,66(10):1755-1765
The aim of this study was to investigate the contribution of mitochondrial dysfunction to chemoresistance and migration of
hepatoma cells. We found that inhibition of mitochondrial respiration and mitochondrial DNA (mtDNA) depletion resulted in
induction of amphiregulin (AR) expression in HepG2 cells. Upon oligomycin treatment of HepG2 cells, the cytosolic Ca2+ was significantly raised after 30 min, and the intracellular level of reactive oxygen species (ROS) was elevated 2.2-fold
after 4 h. Moreover, the condition medium of oligomycin-treated HepG2 cells was found to stimulate the migration of SK-Hep-1
cells. On the other hand, oligomycin-induced cisplatin-resistance and cell migration of HepG2 cells were attenuated by AR-specific
RNA interference (#L-017435, Dharmacon) and a neutralizing antibody (MAB262, R&D Systems), respectively. Together, these findings
suggest that mitochondrial dysfunction induced Ca2+ mobilization, and ROS overproduction, which modulated the chemo-resistance and migration of hepatoma cells through the induction
and activation of AR.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Y.-H. Wei, H.-C. Lee: These authors contribute equally to this work.
Received 02 December 2008; received after revision 16 March 2009; accepted 17 March 2009 相似文献
7.
8.
Arachiche A Badirou I Dachary-Prigent J Garcin I Geldwerth-Feniger D Kerbiriou-Nabias D 《Cellular and molecular life sciences : CMLS》2008,65(23):3861-3871
Rapid Ca2+-dependent phospholipid (PL) reorganization (scrambling) at the plasma membrane is a mechanism common to hematopoietic cells
exposing procoagulant phosphatidylserine (PS). The aim of this research was to determine whether activation of the extracellular
signal-regulated kinase (ERK) pathway was required for PL scrambling, based on a single report analyzing both responses induced
by Ca2+ ionophores in megakaryoblastic HEL cells. Ca2+ ionophore-stimulated ERK phosphorylation was induced in platelets without external Ca2+, whereas exogenous Ca2+ entry was crucial for ERK activation in Jurkat T cells. In both cells, membrane scrambling only occurred following Ca2+ entry and was not blocked by inhibiting ERK phosphorylation. Furthermore, ERK proteins are strongly phosphorylated in transformed
B lymphoblastic cell lines, which do not expose PS in their resting state. Overall, the data demonstrated that ERK activation
and membrane scrambling are independent mechanisms.
A. Arachiche, I. Badirou: These authors contributed equally to this work.
Received 18 June 2008; received after revision 24 September 2008; accepted 1 October 2008 相似文献
9.
Cell-cell adhesion is a critical property of all multi-cellular organisms and its correct regulation is critical during development,
differentiation, tissue building and maintenance, and many immune responses. The multi-talin-like FERM domain containing protein,
FrmA, is required during starvation-induced multi-cellular development of Dictyostelium cells. Loss of FrmA leads to increased cell-cell adhesion and results in impaired multi-cellular development, slug migration
and fruiting bodies. Further, mixing experiments show that FrmA null cells are excluded from the apex of wild-type mounds,
to which cells that normally form the organising centre known as the tip sort. These data suggest a critical role for FrmA
in regulating cell-cell adhesion, multi-cellular development and, in particular, the formation of the organising centre known
as the tip.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Received 28 August 2008; received after revision 10 October 2008; accepted 21 October 2008 相似文献
10.
Lan Chen Yan Wei Xueqing Wang Rongqiao He 《Cellular and molecular life sciences : CMLS》2009,66(15):2559-2571
Although the glycation of Tau that is involved in paired helical filament formation in Alzheimer’s disease has been widely
studied, little attention has been paid to the role of d-ribose in the glycation of Tau. Here, we show that Tau is rapidly glycated in the presence of d-ribose, resulting in oligomerization and polymerization. Glycated derivatives appeared after 24 h incubation. Western blotting
indicated the formation of advanced glycation end-products (AGEs) during initial stages of glycation. Thioflavin T-positive
(ThT-positive) aggregations that appeared from day 4 indicated the globular-like features. Atomic force microscopy revealed
that the surface morphology of ribosylated Tau40 was globular-like. Kinetic studies suggested that d-ribosylated Tau is slowly oligomerized and rapidly polymerized with ThT-positive features. Moreover, d-ribosylated Tau aggregates were highly toxic to SHSY5Y cells and resulted in both apoptosis and necrosis. This work has demonstrated
that d-ribose reacted with Tau protein rapidly, producing ThT-positive aggregations which had high cytotoxicity.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
11.
Wang Y Guan X Fok KL Li S Zhang X Miao S Zong S Koide SS Chan HC Wang L 《Cellular and molecular life sciences : CMLS》2008,65(23):3822-3829
Rhomboid family members are widely conserved and found in all three kingdoms of life. They are serine proteases and serve
important regulatory functions. In the present study, a novel gene highly expressed in the testis, RHBDD1, is shown to be
a new member of the Rhomboid family, participating in the cleavage of BIK, a proapoptotic member of the Bcl-2 family. The
RHBDD1-involved proteolytic modification is upstream of the BIK protein degradation pathway. Mutagenesis studies show that
the amino acid residues glycine142 and serine144 of RHBDD1 are crucial for its activity in cleaving BIK at a site located
in the transmembrane region. Overexpression or knock-down of RHBDD1 in HEK 293T cells can reduce or enhance BIK-mediated apoptosis,
respectively. The present findings suggest that, by acting as a serine protease, RHBDD1 modulates BIK-mediated apoptotic activity.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Received 31 July 2008; received after revision 16 September 2008; accepted 19 September 2008 相似文献
12.
L. Yin C. M. Chung R. Huo H. Liu C. Zhou W. Xu H. Zhu J. Zhang Q. Shi H. Y. C. Wong J. Chen Y. Lu Y. Bi C. Zhao Y. Du M. Ma Y. Cai W. Y. Chen K. L. Fok L. L. Tsang K. Li Y. Ni Y. W. Chung Z. Zhou J. Sha H. C. Chan 《Cellular and molecular life sciences : CMLS》2009,66(5):900-908
The acrosome reaction has long been thought to be induced by the zona pellucida. Here we report the identification and function
of a novel human sperm glycosylphosphatidylinositol (GPI)-anchored membrane protein, NYD-SP8. The release of the protein during
sperm-egg interaction and its binding to the cumulus, the first layer of egg investment, elicits cross-talk between the gametes
and produces calcium dependant release of progesterone, which lead to the acrosome reaction. An in vivo mouse model of NYD-SP8 immunization is also established showing a reduced fertility rate. Thus, contrary to accepted dogma,
our study demonstrates for the first time that, prior to reaching the zona pellucida, sperm may release a surface protein
that acts on the cumulus cells leading to the acrosome reaction, which may be important for determining the outcome of fertilization.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Received 11 August 2008; received after revision 18 December 2008; accepted 22 December 2008 相似文献
13.
F. Magherini A. Carpentieri A. Amoresano T. Gamberi C. De Filippo L. Rizzetto M. Biagini P. Pucci A. Modesti 《Cellular and molecular life sciences : CMLS》2009,66(5):933-947
In this study, a proteomic approach that combines selective labelling of proteins containing reduced cysteine residues with
two-dimensional electrophoresis/mass spectrometry was used to evaluate the redox state of protein cysteines during chronological
ageing in Saccharomyces cerevisiae. The procedure was developed on the grounds that biotinconjugated iodoacetamide (BIAM) specifically reacts with reduced cysteine
residues. BIAM-labelled proteins can then be selectively isolated by streptavidin affinity capture. We compared cells grown
on 2% glucose in the exponential phase and during chronological ageing and we found that many proteins undergo cysteine oxidation.
The target proteins include enzymes involved in glucose metabolism. Both caloric restriction and growth on glycerol resulted
in a decrease in the oxidative modification. Furthermore, in these conditions a reduced production of ROS and a more negative
glutathione half cell redox potential were observed.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Received 15 September 2008; received after revision 17 December 2008; accepted 06 January 2009 相似文献
14.
A. Hamadi T. B. Deramaudt K. Takeda P. Rondé 《Cellular and molecular life sciences : CMLS》2009,66(2):324-338
Cell migration requires the coordinated turnover of focal adhesions, a process that involves FAK phosphorylation. Since Src
is the major kinase implicated in FAK phosphorylation, we focus here on the role of Src activation on adhesion remodelling.
In astrocytoma cells, constitutively activated Src induces both FAK phosphorylation and adhesion rearrangement. To evaluate
how Src controls these processes, we used a recently described Src reporter to monitor the dynamics of Src phosphorylation.
Upon Src activation, focal adhesions started to disassemble while Src appeared highly expressed at newly formed membrane ruffles.
Kinetic analysis of time-lapse movies showed that loss of phospho-Src at focal adhesions was time-correlated with the appearance
of membrane ruffles containing phospho-Src. Moreover, FLIP analysis revealed a dynamic equilibrium of Src between focal adhesions
and membrane ruffles. We conclude that upon phosphorylation, Src is directly translocated from focal adhesions to membrane
ruffles, thereby promoting formation of new adhesion complexes.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Received 21 July 2008; received after revision 10 October 2008; accepted 03 November 2008 相似文献
15.
Two major functions of the Golgi apparatus (GA) are formation of complex glycans and sorting of proteins destined for various
subcellular compartments or secretion. To fulfill these tasks proper localization of the accessory proteins within the different
sub-compartments of the GA is crucial. Here we investigate structural determinants mediating transition of the two glycosyltransferases
β-1,4- galactosyltransferase 1 (gal-T1) and the α-1,3-fucosyltransferase 6 (fuc-T6) from the trans-Golgi cisterna to the trans-Golgi network (TGN). Upon treatment with the ionophore monensin both glycosyltransferases are found in TGN-derived swollen
vesicles, as determined by confocal fluorescence microscopy and density gradient fractionation. Both enzymes carry a signal
consisting of the amino acids E5P6 in gal-T1 and D2P3 in fuc-T6 necessary for the transition of these glycosyltransferases from the trans-Golgi cisterna to the TGN, but not for their steady state localization in the trans-Golgi cisterna.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Received 30 July 2008; received after revision 17 September 2008; accepted 29 September 2008 相似文献
16.
S Roberts 《Experientia》1985,41(9):1138-1139
The distribution patterns of collagen types I, II and III were studied using immunofluorescent staining techniques in human articular cartilage, including the calcified layer. Tissue taken from femoral heads was stained with the appropriate antiserum. Adjacent sections were stained with von Kossa or Alizarin red to determine the distribution of calcium salts. Results indicate that endochondral ossification at this site occurs by calcium being deposited initially within a matrix of type II collagen. 相似文献
17.
S. Roberts 《Cellular and molecular life sciences : CMLS》1985,41(9):1138-1139
Summary The distribution patterns of collagen types I, II and III were studied using immunofluorescent staining techniques in human articular cartilage, including the calcified layer. Tissue taken from femoral heads was stained with the appropriate antiserum. Adjacent sections were stained with von Kossa or Alizarin red to determine the distribution of calcium salts. Results indicate that endochondral ossification at this site occurs by calcium being deposited initially within a matrix of type II collagen. 相似文献
18.
E. A. E. van Marck S. Stocker J. A. Grimaud L. Kestens P. L. J. Gigase A. M. Deelder 《Cellular and molecular life sciences : CMLS》1980,36(9):1116-1118
Summary An experimental model of schistosomal portal fibrosis is described. Sepharose beads the size of schistosome eggs, loaded or not with soluble egg antigen (SEA) fromSchistosoma mansoni, are injected into the coecal vein of C3H/Sn mice and become embolized in the liver. Only SEA-coated beads evoke a granulomatous reaction; this is enhanced by simultaneous priming of the mice with spleen cells fromSchistosoma mansoni-infected syngeneic animals. The fibrosis, which ensues around the beads, is stable and is much more evident after priming. Preliminary collagen tissue immunotyping reveals the presence of collagen deposits of types I and III collagen. Type IV collagen remains unchanged in the portal tracts. The model appears to be well suited for studies of the pathogenesis of portal fibrosis.This work was supported by a contract from INSERM (Action Spéciale No 3). 相似文献
19.
R. George H.-L. Chan Z. Ahmed K. M. Suen C. N. Stevens J. A. Levitt K. Suhling J. Timms J. E. Ladbury 《Cellular and molecular life sciences : CMLS》2009,66(4):711-720
The three isoforms of the adaptor protein Shc play diverse roles in cell signalling. For example, the observation of p46 Shc
in the nuclei of hepatocellular carcinoma cells suggests a function quite distinct from the better characterised cytoplasmic
role. Ligands responsible for the transport of various Shc isoforms into organelles such as the nucleus have yet to be reported.
To identify such ligands a far western approach was used to determine the p52 Shc interactome. The Ran-GTPase nuclear transport
protein was identified and found to bind to p52 Shc in vitro with low micromolar affinity. Co-immunoprecipitation, pull down and fluorescence lifetime imaging microscopy experiments
in stable cells confirmed cellular interaction and nuclear localisation. The nuclear transport factor protein NTF2, which
functions in cohort with Ran, was shown to form a complex with both RAN and Shc, suggesting a mechanism for Shc entry into
the nucleus as part of a tertiary complex.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Received 20 October 2008; received after revision 04 December 2008; accepted 15 December 2008 相似文献
20.
H Lesot K von der Mark J V Ruch 《Comptes rendus des séances de l'Académie des sciences. Série D, Sciences naturelles》1978,286(10):765-768
The different types of collagen synthesized by embryonic Mouse tooth germs were analysed using an immunohistiological method. The tooth germs were shown to synthesize at least three genetically distinct collagen types: I, III and IV. 相似文献