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正由于人体器官供应不足,心力衰竭等有器官移植需求的患者往往在漫长的等待过程中离开人世。因此,科学家们很早就开始研究异种移植(xenotransplantation)。一项于2018年12月5日发表在Nature上的研究显示,一只狒狒在移植了基因改造的猪心脏后成功存活了195天。这意味着在不 相似文献
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异种器官移植的研究进展 总被引:2,自引:0,他引:2
器官移植已成为许多终末期疾病首选的治疗方法。全世界至今接受心、肝、肾等移植者已达 6 0余万例。随着外科手术的成功 ,免疫抑制剂的不断发展 ,移植成功率的提高 ,出现了供体严重短缺现象 ,异种移植已成为研究的方向。目前 ,只有 1 2的患者有机会接受移植 ,大约有 1 5的患者在等待中死亡。等待器官移植时间越长 ,患者死亡机率越大。因此 ,供者器官来源不足严重困扰着器官移植手术的推广和应用。跨物种异种移植成为医学领域亟待解决的问题。1 异种移植的创立与发展1 1 动物器官移植的特点异种移植 (Xenotransplantatio… 相似文献
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猪内源性逆转录病毒与异种移植安全性问题 总被引:3,自引:0,他引:3
异种移植是将动物的活细胞、组织或器官移植给其它种类受者,其临床应用可以有效缓解同种供体移植物短缺,治疗人类终末期疾病。猪来源方便,易于繁育,与人体解剖和生理有很高的相似性,可选择合适大小的器官,因此成为异种移植较好的供体源。猪胰岛细胞临床应用治疗胰岛素依赖型糖尿病[1]、猪肾体外灌注用于治疗肾衰[2]、猪生物人工肝用于治疗人肝坏死[3]、猪心瓣用于治疗人心脏病[4]、猪神经细胞用于治疗人帕金森病[5],这些尝试给异种移植带来希望。然而,异种移植面临三个主要障碍,即移植物进入受者体内引发的免疫排斥反应,包括超急性排斥反应… 相似文献
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田地 《大众科学.科学研究与实践》1997,(4)
最近,国外科学家用转基因猪的心脏对动物做移植试验获得了成功。也许在不久的将来有可能实现用猪心置换人心。迄今,人体器官移植有了长足的发展,但也面临几个急需解决的问题。 相似文献
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目的:比较辐照巴马香猪无细胞真皮与辐照人无细胞真皮移植动物实验效果.方法:以机械去表皮法制备无细胞真皮与人无细胞真皮辐照(剂量30 KGy)处理后移植于W istar大鼠全厚皮肤缺损创面进行比较.结果:辐照处理的巴马香猪无细胞真皮与人无细胞真皮在大鼠创面愈合过程相似,愈合后创面收缩率差异不显著.结论:辐照处理的巴马香猪无细胞真皮与辐照处理的人无细胞真皮移植于W istar大鼠全厚皮肤缺损创面恢复效果无显著差异,可作为人无细胞真皮的替代品进一步用于烧伤治疗的研究. 相似文献
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孙毅 《科技情报开发与经济》2008,18(35):94-96
论述了用生物聚合物工程开发新型塑料、全新的鱼类生物工程研究、人体器官移植研究、仿生眼的研制以及用细胞移植代替器官移植研究的新进展。 相似文献
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随着科学技术日新月异的发展.人类已经可以象机器更换零件那样,更换病态的甚至坏死的人体组织器官。但是,在移植器官的过程中出现的排斥反应,使被移植的器官坏死并危及到受体的生命安全。排斥反应是器官移植的“超级杀手”,曾夺走了成千上万患者的生命。能否找到一种保护器官移植患者生命的方法呢?能!一位女性医学专家以她的科研成果作出了肯定的回答,她叫萧露露。广州血液中心器官移植配型中心主任萧露露和她的同 相似文献
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Xenozoonoses in xenotransplantation 总被引:2,自引:0,他引:2
Pig to human xenotransplantation is one of the possible ways to solve the problem of organ shortage, but the potential risk of xenozoonoses hinders the progress of xenotransplantation. Among pathogens that might cause xenozoonoses, porcine endogenous retrovirus (PERV) is undoubtedly the most noticeable. Current researches show that PERV exists in pigs' genomes and is able to express in multiple tissues and organs in pigs. Other experiments also show that PERV could successfully infect several human cell lines in vitro and could infect SCID mice through pig to mouse islet transplantation. The discussion and research on the risk of PERV is one of the hottest topics in current xenotransplantation, but the question of whether PERV will transmit to human through xenotransplantation has not been answered due to the lack of suitable animal models. More work should be done to evaluate the risk of xenozoonoses caused by PERV after pig to human xenotransplantation. 相似文献
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美国最近连续报道4例基因工程猪供给人的异种器官移植,这些进展打破了异种移植临床研究领域近30年的沉静。尤其是实验性的猪心脏移植取得初步成功,引发了广泛的关注。异种移植面向未来的临床应用迈出了关键性一步。这4个案例,无论是创新精神、技术进步、审批流程,还是伦理学的同步跟进,都值得我国同行学习和借鉴。30年来,基因工程(GE)动物器官已经能在非人类灵长类动物(NHPs)模型中长时间存活。这些实验中,预存抗体、补体激活、凝血状态等机制参与的超急性排异反应基本上得到有效控制。异种移植已经达到临床应用的起跑点。然而,未来仍然有四大问题需要解决:免疫排斥、生物安全、跨种适配和伦理心理。本文简要概括临床异种移植百年来的主要进展,其困难程度可见一斑,但希望和探索从未终止。 相似文献
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Infection by porcine endogenous retrovirus after islet xenotransplantation in SCID mice 总被引:56,自引:0,他引:56
van der Laan LJ Lockey C Griffeth BC Frasier FS Wilson CA Onions DE Hering BJ Long Z Otto E Torbett BE Salomon DR 《Nature》2000,407(6800):90-94
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To identify the genes associated with cellular rejection in pig-to-human xenotransplantation, the suppression subtractive hybridization (SSH) was used in screening the up-regulated genes from a co-culture of human peripheral blood mononuclear cells (PBMCs) and porcine vascular endothelial cell line PIEC. The up-regulated cDNAs were cloned into pGEM-T Easy vector and then sequenced. Nucleic acid homology searches were performed using the BLAST program. A subtracted cDNA library including about 300 clones with the expected up-regulated genes was obtained. Twenty-four of these clones were analyzed by sequencing and homology comparison was made. These clones represent the genes of human perforin (PRF1), proteasome, lymphocyte specific interferon regulatory factor/interferon regulatory factor 4 (LSIRF/IRF 4), muscleblind-like (MBNL) protein and a porcine expressed sequence tag (EST) which has 81% homology with human oxidative-stress responsive 1 (OSR 1). These genes might be the candidate genes which are associated with cellular rejection in pig-to-human xenotransplantation. 相似文献
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胚胎干细胞是一类多能性干细胞,近年来已成为生命科学研究领域的热点之一.尤其在人类疾病治疗方面有着诱人的应用前景.本文主要介绍了胚胎干细胞在几种疑难疾病治疗上的应用及其前景,胚胎干细胞与异种器官移植。以及目前存在的一些问题. 相似文献
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Biotechnology: surrogate broodstock produces salmonids 总被引:1,自引:0,他引:1
A worldwide decline in the number of wild salmonids calls for strategies to restore endangered populations. Here we show that germ cells can be transplanted between two different salmonid species, with the subsequent production of xenogenic, donor-derived offspring. This pioneering xenotransplantation technology may eventually find applications in facilitating the production of commercially valuable fish, as well as in species conservation. 相似文献
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In order to investigate whether the non-classical HLA-G class I molecule protects the prcine endothelial cells(PECs)from the lysis mediated by human immune cells in pig to human discordant xenotransplantation,we have cloned HLA-G cDNA from a human placents by RT-PCR.Mammalian expression vector,pEFG-neo,was constructed by insertion of HLA-G cDNA in pEF-neo.We obtained efficiently expressed PECs by stable transfection.Cytotoxicity assay showed that overexpression of HLA-G on PECs was sufficient to inhibit human NK-92 cell lysis.The level of lysis was equal to or less than that of the lysis of human umbilical vein endothelial cells mediated by human NK-92 cells.It also indicated that HLA-G inhibited the lysis of PECs mediated by xeno-antigen specific T lymphocytes.The reduction of lysis ranged between 59.1% and 88.9A%.These findings suggest that the transgenic approach to overexpress HLA-G is believed to be a new immunotherapy in overconing the immune rejections in xenotransplantion,including delayed xenograft rejection and cell-mediated rejection. 相似文献
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SHI Bin YIN Huijun HUANG Xiuying & SUN Fangzhen Institute of Genetics Developmental Biology Chinese Academy of Sciences Beijing China Correspondence should be addressed to Sun Fangzhen 《科学通报(英文版)》2003,48(2)
The worldwide shortage in the supply of human do-nor organs is becoming more and more pronounced. Xenotransplantation may probably give the hope to over-come the problem ultimately. Because it has a great pros-pect of clinical application, xenotransplantation has drawn great attention[1]. The pig appears to be an ideal source for human transplantation. But the xenograft has to face the challenge of three severe rejections (the hyperacute rejec-tion, the delayed xenograft rejection and acute ce… 相似文献
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Cloned pigs derived from somatic cell nuclear transfer embryos cultured in vitro at low oxygen tension 总被引:4,自引:0,他引:4
PAN Dengke ZHANG Yunhai SUN Xiuzhu ZHANG Jian LI Xuyang LI Yan GU Zhiliang DA Yunping WU Changxin LI Ning 《科学通报(英文版)》2006,51(7):839-844
Great progress have been made in animal cloning in China, as evidenced by the live births of cloned cat- tle[1,2], goats[3,4], and sheep[5]. In contrast, pig cloning is still in its infancy though limited fundamental studieshave been conducted[6]. It is g… 相似文献