荒漠沙蜥血清蛋白含量的年周期变动

对荒漠沙蜥（Phrynocphalus przewalskii)血清蛋白仿及血清蛋白组分的周期变化进行了测定，测定时间为4－10月的每月中旬及1月中旬（进行人工冬眠的蜥蜴），血清总蛋白含量采用紫外分光光度法测定，血清蛋白组合组分采用醋酸纤维素薄膜电泳分离，然后用双波长色谱扫描仪扫描，计算相对含量，测定结果表明，荒漠沙蜥的血清总蛋白含量在深冬眠期的1月最高，为81．45g/L，4月出 一进入繁殖季节，其含量开始下降，夏季（6－8月）降为43．81g/L，其中7月最低，为38．07g／L，秋季的含量略低于春季的含量，但差异不显著（P＞0．05），在冬 眠前（10月下旬）及冬眠期（1月中旬），雌性的含量高于雄性，且差异显著（P＜0．05），在出眠后的繁殖季节雄性的含量高于雌性，在冬眠季节清蛋白的比例相对增加，而球蛋白的比例相对减少。     

西藏嘉黎,亚东牦牛血清蛋白含量及组成分析

采用Ｂｒａｄｆｏｒｄ比色法测定西藏嘉黎、亚东两地区牦牛血清总蛋白含量，通过醋酸纤维薄膜电泳分离其血清蛋白各组成，并以ＣＤＳ２００型计算机控光密度仪进行扫描．测得４９头嘉黎牦牛的血清总蛋白含量为６．２８±０．３４ｇ／１００ｍｌ血清，其中各组成的百分含量分别是：白蛋白４５．５９±０．９２，α１球蛋白１７．０６±０．４０，α２球蛋白８．９７±０．６８，β球蛋白１０．５１±０．５０，γ球蛋白１７．７５±０．７０，清球蛋白比值（Ａ／Ｇ）为０．８４±０．０２；３６头亚东牦牛的血清总蛋白含量为６．４５±０．２０ｇ／１００ｍｌ血清，其中各组成的百分含量分别是：白蛋白４６．６６±１．１０，α１球蛋白１７．４８±１．０２，α２球蛋白８．８５±０．６６，β球蛋白９．６３±０．７４，γ球蛋白１７．４２±０．９８，清球蛋白比值（Ａ／Ｇ）为０．８８±０．０４．经ｔ检验分析，两地区牦牛血清蛋白组成中只有β球蛋白含量具有显著差异（Ｐ＜０．０５），其余均无显著性差异（Ｐ＞０．０５）．     

重金属Hg^2＋对NBS修饰前后血清蛋白圆二色谱的影响

为了探明重金属Hg^2＋进入体内，对血清蛋白固有结构和功能的影响，我们用Hg^2＋对NBS修饰前后血清蛋白HSA和BSA进行处理，然后分析血清蛋白的圆二色谱特性，结果表明，近紫外区未修饰的HSA和BSA均具有典型的α螺旋构象；Hg^2＋处理或NBS修饰BSA溶液，均引起α-螺旋含量减少和三级结构构象的改变．Hg^2＋处理HSA，α-螺旋含量略有增加，构象也有变化．而NBS修饰导致α簇结构的严重破坏，蛋白构象无序化程度加深．修饰对BSA、HSA光谱特性的影响不同，可能与这两种蛋白的结构差异有关．     

复合氨基酸营养价值及对小鼠体内酶活性的影响

以豆类蛋白水解液中提取的复合氨基酸为原料，小白鼠为对象，研究复合氨基酸的营养价值及对小鼠体内酶活性的影响。结果表明它有调节小鼠体内酶活性的作用。可使ＳＯＤ、过氧化氢酶和精氨酸酶的活性升高，乳酸脱氢酶活性降低，蛋白质的表观消化率、食物转化效能及血红蛋白和血清蛋白总量提高。     

复事氨基酸营养价值及对小鼠体内酶活性的影响

以豆类蛋白水解液中提取的复合氨基酸为原料，小白鼠为对象，研究复合氨基酸的营养价值及对小鼠体内酶活性的影响，结果表明它有调节小鼠体内酶活性的作用，可使ＳＯＤ、过氧化氢酶和精氨酸酶的活性升高，乳酸脱氢酶活性降低，蛋白质的表观消化率，食物转化效能及血红蛋白和血清蛋白总量提高。     

微波对K562/MDR细胞凋亡及热休克蛋白70表达的影响

研究微波引起白血病耐药细胞株K562／MDR的凋亡以及对热休克蛋白70表达的影响。使用MTT方法检测细胞存活率，AnnexinV/PI双参数法检测K562／MDR细胞的凋亡率，流式细胞仪检测热休克蛋白70的表达。结果表明，微波可起K562／MDR细胞的凋亡，经0.20,0.60W/cm^2功率密度微波作用20min（保持43℃），37℃培养24h后，细胞凋亡率分别为10％、13．6％，而水浴加热引起的凋亡率仅为3．8％，微波照射影响细胞内热休克蛋白70的表达，在43℃时，0.20,0.60W/cm^2功率密度微波作用20min抑制HSP70的表达，实验说明微波能引起K562／MDR细胞的凋亡，该凋亡与微波引起的热休克蛋白表达的改变可能有联系。     

红,白骆驼血清蛋白的比较研究

采用定氮法、电泳法及氨基酸自动分析仪检测红驼和白驼血清蛋白质的各组分含量、分子量及水解氨基酸的种类．结果表明红驼和白驼血清蛋白组成基本一致．薄膜电泳分离得到的５条蛋白区带没有显著差异，ＳＤＳ－ＰＡＧＥ分离均可得到分子量在０．８０×１０４～１１．２×１０４范围内的８条蛋白区带，这些蛋白共由１７种氨基酸按一定的百分比构成．二者的差异主要表现在不同氨基酸种类的含量和高分子量蛋白区带的分布状态．揭示了双峰驼在定向培育过程中血清蛋白表型发生的变异程度     

性别因素对大鼠部分血液学,血液生化指标和脏器系数的影响

为探讨性别因素对大鼠部分生理指标的影响，本实验分别测定了３月龄Ｗｉｓｔａｒ（２０只）和ＳＤ（２０只）大鼠的１０项血液学、８－９项血液生化指标和６项脏器系数，所测结果在同品种不同性别之间比较，结果表明，雄性大鼠血液红细胞、血红蛋白和血球比积值较高；血清丙氨酸氨基转换酶、天冬氨酸氨基转换酶及碱性磷酸酶含量较高，而总蛋白和白蛋白含量较低；雌性大鼠脑和肺的相对重量较高．     

国产氧化型染发剂实验研究

用国产氧化型染发剂对AKR小白鼠染毒实验研究,发现红细胞及血红蛋白下降,血小板尤其明显,白细胞略有上升。鼠肝脏有不同程度的病理改变。对小白鼠胎儿有致死、致畸作用。染发剂对TA98有明显致突变性。     

健康麋鹿血清总蛋白、白蛋白和球蛋白含量的分析

为了解健康麋鹿在不同发育阶段血清中白蛋白和球蛋白含量的变化,采用全自动生化分析仪测定血清中总蛋白、白蛋白和球蛋白的含量.结果表明:雌性和雄性麋鹿血清总蛋白、白蛋白和球蛋白的含量在幼年期较低,随着年龄的增长,蛋白含量在青年期和成年期达到最高水平,成年期后随着年龄的增长逐渐降低.年龄与血清总蛋白、白蛋白和球蛋白的相关性较强,性别与总蛋白、球蛋白和白蛋白的含量相关性差.青年期和成年期的蛋白含量相近,均显著高于幼年期和老年期,差异具有统计学意义(p0.05).在麋鹿发育的各个阶段,雄性血清的蛋白含量均略高于雌性.     

Heat-shock induced proteins present in the cell nucleus of Chironomus tentans salivary gland.

The heat shock (HS) system has been largely studied in Drosophila but the molecular mechanisms responsible for the induction of the heat shock genes as well as the function(s) and the intracellular localisation of the induced proteins is still unknown. It has previously been shown that the HS puff induction is accompanied by a local increase of nuclear nonhistone proteins (NHP) but the nature of most of the proteins accumulating is unknown. We have investigated the effects of a heat shock on Chironomus tentans salivary glands, a system where it is possible to study constituents in various subcellular or intranuclear regions including individual puffs, by microdissection. We report here evidence that at least two of the polypeptides synthesised in response to the heat shock migrate to the nucleus. Furthermore, these two proteins appear to have a broad intranuclear distribution, as shown by their presence in the various microdissected nuclear fractions.     

The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I

Many specific sites in Drosophila chromatin are hypersensitive to DNase I. The positions of such sites were mapped along the regions of the genome coding for two heat shock proteins. Such sites lie at the 5' ends of heat shock genes and may function as elements for recognition by molecules which regulate gene activity.     

棉花抗热性与高温诱导可溶性蛋白质关系

为鉴定早期棉花基因型抗热性提供生理指标,选用抗虫棉品种DP,彩色棉品种LV和ZH,研究了棉花萌动期、子叶期、1叶期和自然高温期热激处理与对照可溶性蛋白含量的差异.结果表明:萌动期、子叶期、1叶期热激和自然高温期,DP可溶性蛋白质总含量分别比对照提高10.24%、13.11%、15.09%和74.36%;LV分别比对照提高4.27%、8.47%、16.48%和53.49%;ZH分别比对照提高15.83%、11.22%、17.86%和18.18%.     

热休克蛋白的研究进展

在不利的环境中,各种有机体都有其共同对应的分子反应,即正常基因的表达抑制和一组特殊基因——热休克基因的激活和表达,导致热休克蛋白的大量产生,热休克蛋白主要作为分子伴侣而参与蛋白质的折叠、转运及组装等过程,能恢复或加速清除细胞内已变性的蛋白质而稳定细胞结构,细胞产生热耐受。随着对热休克蛋白研究的不断深入,在生物工程和医学等方面的应用前景十分广阔。     

植物热激蛋白研究进展

热激蛋白是一类在有机体受到高温等逆境刺激后大量表达的蛋白,主要参与生物体内新生肽的运输、折叠、组装、定位以及变性蛋白的复性和降解,是细胞内含量最丰富的蛋白质之一,已成为当今分子生物学、蛋白质生物化学和植物抗逆生理学的一个重要研究内容.论述了植物热激蛋白的研究概况、合成及分布、生物学功能、基因表达调控,提出植物热激蛋白今后的研究方向。     

热击对水稻(O.sativaL.)叶绿素a,b及POR同功酶的效应

研究热击对水稻苗期生理生化的影响,结果表明,在热击条件下（３５℃,４０℃,４５℃各半个小时）水稻苗期生长出现显著差异,特别是经过热击和非热击条件对比更加明显。此外,经热击处理后,水稻苗期叶绿素的含量随温度的不同而呈现一定的变化规律,并且经聚丙烯酰胺凝胶电泳发现,热击对过氧化物酶同工酶产生显著影响。     

Proteomics study of the effects of high pigment-1 on plastid differentiation during the ripening of tomato fruits

The chloroplast-to-chromoplast difference is a highly regulated process. Tomato （Solanum lycopersicum） high-pigment mutant hp-1 shows an enhanced accumula- tion of carotenoids coupled with an increase in the volume and number of plastids. However, how chromoplasts of hp-] acquire their specific metabolic characteristics is still unclear. A comparison of proteome profiles from plastids at the mature green, breaker, and red stages of tomato fruits showed 45 differentially expressed proteins. These identi- fied proteins fell into six different functional categories. Our results showed that most of the proteins related to the Calvin cycle increased transitorily only at the early breaker stage, and remained unchanged at the early red stage. We found 18 proteins that were differentially expressed between the wild type and hp-1. The abundance of GCPE in chromoplasts of hp-1 was higher than that in the wild type. Meanwhile, we found that heat shock proteins were only present specifically in the mature green stage chromoplasts of hp-1 but not in the wild type. This suggested that GCPE and heat shock proteins might play important roles in the accumulation of high carotenoids in hp-1. Overall, our results could be helpful for understanding chloroplast-to-chromoplast differentiation and the function of hp-1.     

长爪沙鼠的血液及血清生化测定值

分析了３０只长爪沙鼠的血液指标及血清生化指标,包括红细胞（ＲＢＣ）、白细胞（ＷＢＣ）、血色素（ＨＧＢ）血小板（ＰＬＴ）及白细胞分类,血糖（ＧＬＵ）尿素氮（ＢＵＮ）胆红素（ＢＩＬ）,胆固醇（ＣＨＯ）,总蛋白（ＴＰ）及蛋白电泳,结果表明,总蛋白ＴＰ）,血糖（ＧＬＵ）胆红素（ＢＩＬ）、血色素（ＨＧＢ）、红细胞压积（ＨＣＴ）结果都接近大鼠相应参考值。     

Cyclic AMP response element binding protein (CREB) participates in the heat-inducible expression of humanhsp90β gene

Human heat shock protein 90b gene ( hsp90b ) is a constitutively expressed heat shock gene existing in most of cell types tested that can be further induced by heat shock. Chloramphenical acetyl transferase (CAT) reporter plasmids driven by different regulatory fragments of hsp90b gene were constructed and transfected into Jurkat cells to explore the role of a cAMP response element (CRE) in the upstream of the gene. Results show that, in comparison with the wild type construct, a severe reduction (~2/3) in the increased folds of promoter activity induced by heat shock at 42℃ for 1 h was observed in a construct with CRE-containing fragment (-173/-91bp) deleted. Electrophoretic mobility shift assays (EMSA) showed that phosphorylated CRE-binding protein (CREB) in the nuclear extract of heat shocked Jurkat cells is specifically bound to the fragment. Additionally, both of the phosphorylation on CREB and the activity of protein kinase A (PKA) were found in Jurkat cells to be enhanced with extending time of heat shock treatment. Our results indicate that in addition to the intronic HSE/HSF pathway, phosphorylated CREB also participates in the heat shock induced expression of human hsp90b gene via its interaction with CRE which may be regulated by PKA-sig- naling pathway.