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1.
J C Chabala V B Waits T Ikeler A A Patchett L Payne L H Peterson R A Reamer K Hoogsteen M Wyvratt W L Hanson 《Experientia》1991,47(1):51-53
1-(Substituted)benzyl-5-aminoimidazole-4-carboxamides are potent orally active inhibitors of Trypanosoma cruzi infections in mice. The most active compounds are the 1-(4-chlorobenzyl)- and 1-(3,4-dichlorobenzyl)-analogs (L-153,094 [2] and L-153,153 [4], resp.) which are approximately 7-fold more potent upon oral administration than nifurtimox (Lampit) in suppressing parasite levels in the blood of mice with acute Trypanosoma cruzi infections. 相似文献
2.
S. G. Goijman J. F. Turrens G. B. Marini-Bettolo A. O. M. Stoppani 《Cellular and molecular life sciences : CMLS》1985,41(5):646-648
Summary Tingenone and horminone, two natural quinonoid substances, inhibited the in vitro growth ofTrypanosoma cruzi, 30 M drug concentration producing total inhibition of growth. Tingenone inhibited total uptake and incorporation of [3H]thymidine, [3H]uridine, L-[3H]leucine into parasite macromolecules. Other quinonoids assayed were either less effective (abruquinone A) or even quite inactive (visminone B and ferruginin B). Investigation of several mechanisms for the cytotoxic action of tingenone pointed to the interaction with DNA as the most likely factor involved. Tingenone also inhibited the growth ofCrithidia fasciculata, but the drug was significantly less active on this organism than onT. cruzi.This work was supported by grants of UNDP/World Bank/World Health Organization Special Programme for Research and Training in Tropical Diseases, Organization of American States (Multinational Programme of Biochemistry) and Programa Nacional de Enfermedades Endémicas (SECYT), República Argentina. A preliminary account was given at the Workshop on Oxidative Damage and Related Enzymes, Frascatti (Italy), 1983. 相似文献
3.
Summary In mice chronically infected withTrypanosoma cruzi, the masseter muscle (rich in type II fibers) was devoid of inflammatory infiltrates and parasites. In contrast, other muscles, composed of type I and II fibers, showed a decrease of type I fibers, parasites and lesions, suggesting that inT. cruzi infection type I muscle fibers are selectively damaged. 相似文献
4.
E. G. Díaz de Toranzo J. A. Castro B. M. Franke de Cazzulo J. J. Cazzulo 《Cellular and molecular life sciences : CMLS》1988,44(10):880-881
Summary Epimastigotes ofTrypanosoma cruzi (Tulahuen strain Tul 0 stock) biotransform benznidazole (N-benzyl-2-nitro-1-imidazone acetamide) to reactive metabolites that bind covalently to DNA, proteins and lipids of the parasite. These effects might be related to the trypanocidal action of benznidazole, a chemotherapeutic agent against Chagas' disease. 相似文献
5.
Summary Immune but not normal T cells were able fully to restore the ability of thymectomized, irradiated, fetal liver reconstituted B mice to controlTrypanosoma cruzi infections.We are indebed to Dr. A.F. Pestana de Castro for his suggestions. 相似文献
6.
P. J. Islip M. V. Bogunović-Batchelor 《Cellular and molecular life sciences : CMLS》1985,41(10):1353-1354
Summary The synthesis and antischistosome properties of 5-(hydroxyimino)-4-methoxy-2-(pivaloylimino)thiazolidine-3-acetamide (1) are described. The compound was prepared by reduction of the nitrothiazoline (2) with stannous chloride in methanol, and represents the first example of a reduced nitroheterocyclic compound showing potent schistosomicidal properties. The possible relationship of compounds such as1 to the as yet unidentified reduced active but toxic entities formed in vivo from nitroheterocyclics like metronidazole is discussed.Acknowledgments. The authors are grateful to Dr A. J. Everett and his staff for the physical chemistry measurements, and to Mr G. Dickerson for the antischistosome testing. 相似文献
7.
A. S. Couto B. Zingales R. M. de Lederkremer W. Colli 《Cellular and molecular life sciences : CMLS》1985,41(6):736-738
Summary Trypomastigote forms (infective) ofTrypanosoma cruzi incorporate (3H)-palmitic acid and D-(3H)-galactose into glycolipids. Palmitic acid-labeled acidic glycolipids were partially hydrolyzed with neuraminidase. The labeling of these compounds when the intact cell surface was labeled with galactose oxidase plus NaB3H4 indicates the membrane location of the sialoglycolipids.This investigation received financial support from SECYT and CONICET to RML; CNPq, FINEP, and UNDP/World Bank/ WHOSSpecial Programme for Research and Training in Tropical Diseases to WC; and FAPESP to BZ and WC. ASC is a fellow from FAPESP. 相似文献
8.
I. L. Pécora G. A. dos Reis M. A. Barcinski D. D. Dorigo 《Cellular and molecular life sciences : CMLS》1980,36(8):942-944
Summary The frequency distribution ofTrypanosoma cruzi inside macrophages from normal of chronically infected resistant and susceptible mice obeys a negative binomial type of distribution. This implies that an aggregating mechanism operates inT. cruzi: macrophage interaction.Acknowledgments. We thank Dr Sylvio T. Torres for providing the inbred strains of mice. The work was supported by the Brazilian Research Council (CNPq) and the Financiadora de Estudos e Projectos (FINEP). 相似文献
9.
G. A. Rosenthal M. A. Berge J. A. Bleiler T. P. Rudd 《Cellular and molecular life sciences : CMLS》1987,43(5):558-561
Summary L-Canavanine, 2-amino-4-(guanidinooxy)butyric acid, and L-arginine incorporation into de novo synthesized proteins was compared in six organisms. Utilizing L-[guanidinooxy14C]canavanine and L-[guanidino14C]arginine at substrate saturation, the canavanine to arginine incorporation ratio was determined in de, novo synthesized proteins.Caryedes brasiliensis andSternechus tuberculatus, canavanine utilizing insects;Canavalia ensiformis, a canavanine storing plant; and to a lesser extentHeliothis virescens, a canavanine resistant insect, failed to accumulate significant canavanyl proteins. By contrast,Manduca sexta, a canavanine-sensitive insect, andGlycine max, a canavanine free plant, readily incorporated canavanine into newly synthesized proteins. This study supports the contention that the incorporation of canavanine into proteins in place of arginine contributes significantly to canavanine's antimetabolic properties. 相似文献
10.
Summary Autolysates ofTrypanosoma congolense, in subcytotoxic amounts, were found to be highly mitogenic in vitro for the spleen cells of normal mice. Significant amounts of [3H]-thymidine were also incorporated by the responding spleen cells of nu/nu (athymic) mice. In contrast, the spleen cells of cyclophosphamide-treated mice were unresponsive. The findings suggest that a potent B-cell-mitogen is generated by the autolysingT. congolense organism.Acknowledgments. This investigation was supported by the International Development Research Centre, Canada. 相似文献
11.
P. A. Barrett Elizabeth Beveridge D. Bull I. C. Caldwell P. J. Islip R. A. Neal N. C. Woods 《Cellular and molecular life sciences : CMLS》1982,38(3):338-339
Summary The biological properties of a novel compound 353C with high activity againstTrypanosoma cruzi, are described. The compound was about 10 times and 20 times more effective than either benznidazole or nifurtimox respectively, in producing radical cure in mice. 353C had a long half-life and showed anti-trypanosomal properties when given to mice at weekly intervals. 相似文献
12.
M. Queiroz da Cruz H. M. Bräscher J. R. Vargens A. Oliveira-Lima 《Cellular and molecular life sciences : CMLS》1991,47(1):89-92
Summary Viable metacyclic forms ofT. cruzi, Y strain, treated with an adequate dose of actinomycin D (50 g Act-D/ml/107 parasites/ml for 72 h at 28° C) showed the following properties: 1) they lost their ability to replicate in culture medium, in blood and in tissues of normal mice and were no longer able to incorporate tritiated thymidine; 2) they could no penetrate into Vero cells and could not replicate inside normal macrophages; 3) they retained their immunogenicity and the ability to protect mice against a virulent infection; 4) they did not induce histological lesions as described in chronic experimental Chagas' disease. 相似文献
13.
Heras SR Thomas MC García-Canadas M de Felipe P García-Pérez JL Ryan MD López MC 《Cellular and molecular life sciences : CMLS》2006,63(12):1449-1460
A comparative analysis of 40 Trypanosoma cruzi L1Tc elements showed that the 2A self-cleaving sequence described in viruses is present in them. Of these elements, 72% maintain
the canonical 2A motif (DxExNPGP). A high percentage has a conserved point mutation within the motif that has not been previously
described. In vitro and in vivo expression of reporter polyproteins showed that the L1Tc2A sequence is functional. Mutations within certain L1Tc2A sequences
affect the efficiency of the cleavage. The data indicate that the L1Tc2A sequence may be influencing the L1Tc enzymatic machinery
determining the composition and level of the translated products. The residues located immediately upstream of the 2A consensus
sequence increase the cleaving efficiency and appear to stabilize the relative amount of translated products.
These authors contributed equally to this work.
Received 26 January 2006; received after revision 11 April 2006; accepted 21 April 2006 相似文献
14.
T. Horio Y. Kawabata T. Takayama S. Tahara J. Kawabata Y. Fukushi H. Nishimura J. Mizutani 《Cellular and molecular life sciences : CMLS》1992,48(4):410-414
A highly potent attractant of zoospores ofAphanomyces cochlioides, a causal fungus of the root rot disease of spinach (Spinacia oleracea), was isolated from spinach roots, and its structure was determined by spectroscopic evidence and chemical synthesis as cochliophilin A (5-hydroxy-6,7-methylenedioxyflavone,1). A chromosorb particle prepared by soaking in solution of1 showed a potent attracting activity toward the zoospores using concentrations of1 above 10–9 or 10–10 M. 相似文献
15.
M. N. Schwarcz de Tarlovsky S. G. Goijman M. P. Molina Portela A. O. M. Stoppani 《Cellular and molecular life sciences : CMLS》1990,46(5):502-505
Several 4-(aminomethylisoxazolyl)-1,2-naphthoquinones inhibited growth and DNA synthesis inTrypanosoma cruzi and stimulated O2 uptake and
generation by the parasite epimastigotes and their mitochondrial and microsomal membranes; these results support the idea that oxygen radicals play a role in quinone toxicity. Maximal effects on respiration and
generation were observed with antimycin-inhibited cells. Similar results as well as stimulation of H2O2 production were obtained withCrithidia fasciculata despite the presence of catalase in this organism.Acknowledgments. This work was aided by grants from the University of Buenos Aires, the Scientific Office of the American States Organization and CEDIQUIFA (Buenos Aires). S.G.G. and M.P.M.P. are Research Fellows and A.O.M.S. is Career Investigator of CONICET (Argentina). L. T. Viñas and M. G. Gutierrez lent able technical assistance. 相似文献
16.
Summary A new norsesterterpene, hyrtial4, and known sesterterpenes,1–3, have been isolated from an anti-inflammatory active crude extract of the spongeHyrtios erecta.This sponge,Hyrtios (Heteronema) erecta Keller 1889, collected in 1980/81, from Tonga was extremely abundant in the Vava'u Is. Group.We thank Prof. R. Jacobs and his research group at UCSB for this data. 相似文献
17.
H. Minakata H. Komura S. Y. Tamura Y. Ohfune K. Nakanishi T. Kada 《Cellular and molecular life sciences : CMLS》1985,41(12):1622-1623
Summary Five unusual amino acids were identified as antimutagens against spontaneous mutation ofSalmonella typhimurium TA100: L-azetidine-2-carboxylic acid (1) from Liliaceae plants, -(methylenecyclopropyl)glycine (2) fromLitchi chinensis seeds, and 2-amino-4-methylhex-5-ynoic acid (3), hypoglycin A (4), and (2S, 4R)-2-amino-4-hydroxyhept-6-ynoic acid (5) fromEuphoria longana seeds. The absolute stereochemistry of5 was determined by its chiral synthesis from L-allylglycine, proving that5 is the C-4 epimer of the amino acid previously isolated from dried longan seeds.This work was partly supported by a Grant-in-Aid form the Ministry of Education, Science, and Culture, Japan, No. 57740283 (to HK). 相似文献
18.
M. Hilker D. Daloze J. M. Pasteels 《Cellular and molecular life sciences : CMLS》1992,48(10):1023-1027
Besides the known sarmentogenin 3-O--D-allopyranoside (1) and digitoxigenin-3-O-[-D-xylopyranosyl-(14)-2, 3-di-O-acetyl--D-allopyranoside] (4), four new cardiac glycosides (2, 3, 5 and6) have been isolated from the defensive glands of adults ofChrysolina fuliginosa. The structures of the new compounds were determined by1H NMR at 600 MHz and FABMS. Compounds1–6, present in the adults ofC. fuliginosa, have also been identified in the eggs. 相似文献
19.
D. Daloze M. Kaisin C. Detrain J. M. Pasteels 《Cellular and molecular life sciences : CMLS》1991,47(10):1082-1089
The composition of the Dufour gland of the antC. scutellaris has been reinvestigated by gas chromatography/mass spectrometry. The major components of the gland are (2E,5E,12Z)-4-oxoheneicosa-2,5,12-trien-1-ol acetate (1a) its
14 and
16 double bond isomers (1b and1c), and the corresponding (Z,Z)-dienes5a and5b, all containing an acetylated C21 chain. The previously proposed structures1d, 1e, and5c, which are based on an homologous acetylated C23 chain, correspond to minor derivatives present in the gland. Traces of acetylated C19 homologs, tentatively identified as1g-1i, have also been found. The Dufour gland contents of the two other EuropeanCrematogaster species have also been studied.C. auberti is very similar toC. scutellaris in producing mainly1a, 1b and1c, together with the same higher and lower homologs, but it lacks the dienic derivatives5, whereasC. sordidula contains essentially the acetylated C19 compounds1g, 1h, and1i, accompanied by acetylated C17 homologs. 相似文献
20.
J. G. Chambó P. M. Cabeza Meckert R. P. Laguens 《Cellular and molecular life sciences : CMLS》1990,46(9):977-979
Summary The existence of antigens shared in common byT. cruzi and heart muscle cells is suggested by the presence of antibodies binding to the parasite surface in the serum of mice with autoimmune myocarditis induced by immunization with syngeneic heart antigens. 相似文献