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1.
The inclusion complex reaction condition and the mechanism of β-cyclodextrin with hymecromone have been studied by fluorescent
spectrum. The fluorescent intensity and the stability of the system can be qreatly increased in qiven condition, and the formation
constant of the inclusion complex reaction has been determined too. The system can be applied to the determination of trace
amounts of hymecromone, the maximum fluorescent intensity is obtained with excitation and emission wavelength at 362 nm and
452 nm, respectively. The linear range for hymecromone is 8.0×10−9 mol·L−1 to 1.0×10−5 mol·L−1. The detection limit is 1.8×10−9 mol·L−1. A simple, rapid and highly sensitive fluorimetric method is proposed for the determination of hymecromone. The results obtained
are in agreement with those given by an official method.
Foundation item: Supported by the National Natural Science Foundation of China (G1999046703)
Biography: Luo Zhao-fu(1947-), female, Associate professor. 相似文献
2.
An electrochemical method for the simultaneous determination of lead(II) and Cadmium(II) with a calix[6]arene modified carbon
paste electrode (CPE) has been developed. Pb2+ and Cd2+ were accumulated at the surface of the modified electrode via formation of chemical complexes with calix[6]arene, and reduced
at 1.40 V. During the following anodic potential sweep, reduced lead and cadmium were oxidized, and two well-defined striping
peaks appeared at about −0.60 V and −0.84 V. Compared with a bare carbon paste electrode, the calix[6]arene modified CPE greatly
improves the sensitivity of determining lead and cadmium. The stripping peak currents change linearly with the concentration
of Pb2+ 3.0×10−8–8.0×10−6 mol·L−1 and with that of Cd2+ 6.0×10−8–1.0×10−5 mol ·L−1. The detection limits of Pb2+ and Cd2+ are found to be 8.0×10−9 mol·L−1 and 2.0×10−8 mol·L−1, respectively. The modified carbon paste electrode was applied to determine trace levels of lead and cadmium in water samples.
Comparing with that of atomic absorption spectrometry, the results suggests that the calix[6]arene modified CPE has great
potential for the practical sample analysis.
Foundation item: Supported by the National Natural Science Foundation of China (60171023)
Biography: JI Xiao-bo (1980-), male, Master, research direction: electroanalytical chemistry. 相似文献
3.
The fluorescence intensity of the europium (III)-tetracycline (TC) complex can be greatly increased by TOPO, TBP, TTA and
surfactans, among which TOPO showed the greatest enhancement. The formation of ternary complex was proposed to be responsible
for the fluorescence enhancement. The Eu (III)-TOPO-TC system can be applied to the determination of trace amounts of tetracycline
in urinous sample; the maximum fluorescence intensity was obtained in the pH range of 8.2∼9.5 with excitation and emission
wave-lengths at 396 nm and 616 nm, respectively. The linear range for tetracycline is 6.0×10−9−5.0×10−7 mol·L−1. The detection limit is 2.0×10−9 mol·L−1(SNR=3). Experimental conditions and the mechanism of the enhancement effect are discussed.
Supported by National Natural Science Foundation of China
Huang Chunbao: born in 1958, Associate professor 相似文献
4.
Highly sensitive voltammetric method for the determination of estrogens, based on the enhancement effect of cetyltrimethylammonium
bromide (CTAB) has been described. In the presence of CTAB, the oxidation peak currents of estrogens (estradiol, estrone,
estriol, estradiol valerate and diethylstilbestrol) as the carbon paste electrode (CPE) increased significantly after open-circuit
accumulation. The peak current was proportional to the concentration of estradiol over the range from 5×10−9 to 2.5×10−6 mol·L−1. The detection limit was 8×10−10 mol·L−1 at 6 min of accumulation. The total amounts of estrogens in the blood serums were determined and the average recovery was
104.92%. Under the conditions used, the electrode process of estradiol was examined the mechanism for peak current enhancement
was also discussed.
Foundation item: Supported by the National Natural Science Foundation of China (60171023) and the Natural Science Foundation
of Hubei Province (99J060)
Biography: Wu Kang-bing(1977-), male, Ph.D. candidate. research direction: bioelectrochemistry. 相似文献
5.
A carbon paste electrode modified with multi-walled carbon nanotubes (MWCNT) was prepared and the determination of ultra trace amount of zirconium based on the anodic adsorptive voltammetry of the zirconium-calcium-alizarin red S mix-polynuclear complex is described in this paper for the first time. The results showed that the sensitivity and the selectivity of the method are excellent. The second derivative linear scan voltammograms of the complex were recorded by polarographic analyzer from 200 to 1200 mV (vs. SCE) and it was found that the complex can be adsorbed on the surface of the electrode, yielding a peak at about 840 mV, corresponding to the oxidation of ARS in the complex. The peak current increases linearly with Zr (IV) concentration in the range of 6.0×10^-12--6.0×10^-11 mol. L^-1 (accumulation time 120 s), 6.0×10^-11--2.0×10^-9 mol. L-1 (accumulation time 90 s) and 2.0×10^-9--1.0×10^-7 mol. L^-1 (accumulation time 60 s) and the detection limit (S/N = 3) is 2.0×10^-12 mol. L^-1 (accumulation time 180 s). The procedure has been successfully applied to the determination of zirconium in the ore samples. 相似文献
6.
SHI Bo’an~ ZENG Danyun~ JI Xinghu~ XI Juan~ AI Xinping~ HE Zhike~ . College of Chemistry Molecular Sciences Wuhan University Wuhan Hubei China . Department of Chemistry Chemical Engineering Hubei Institute for Nationalities Enshi Hubei China 《武汉大学学报:自然科学英文版》2006,11(3):672-676
0 IntroductionThbiep ycroidmipnlee)xru ctahteinoinu mof(Ⅱ r)u t(he Rniuu(mbip,y m)3ai2n +ly) atrnids- t(r2is -,(21’ -,10-phenanthroline)ruthenium(Ⅱ) (Ru(phen)32 +) ,is a kindof sensitive analytical reagent for electrogenerated chemilumi-nescence(ECL) and chemiluminescence(CL) ,on which a par-ticular review has been presented[1]. The earliest publicationon the synthesis of Ru(bipy)32 +appeared in 1936[2], andduringthefollowing30 years ,theinvestigations relatedtothissubstance were only in… 相似文献
7.
CHEN Lihua~ ZHANG Yongkang~ YIN Hong~ LIU Liuzhan~ YANG Zhaoxia~ SHEN Hanxi~ . College of Chemistry Chemical Engineering Jishou University Jishou Hunan China . College of Chemistry Nankai University Tianjin China 《武汉大学学报:自然科学英文版》2006,11(3):704-708
0 IntroductionMaalnadria siusb atr ompajicosr .he aAltrthe pmrisoibnlienmi (n qtihneghtraoopsiucs,QHS,Fig.1) is a sesquiterpene endoperoxide isola-ted fromArtemisia annuaL., an ancient Chineseherbal medicine usedfor treatment of fever and ma-laria.Studies of the structure and activity relation-ship have shownthat endoperoxide groupis essentialfor anti malarial activity of QHS and absence of thismoiety lead to completely loss in activity of thedrug. Many techniques have been developed to de… 相似文献
8.
QIAN Shahua~ LUO Yan~ MO Shaobo~ WENG Yue~ . College of Resources Environmental Sciences Wuhan University Wuhan Hubei China . College of Chemistry Molecular Sciences Wuhan University Wuhan Hubei China 《武汉大学学报:自然科学英文版》2006,11(2):437-440
0Introduction Vanadiumplaysanimportantroleinmodernindustry,es peciallyinsteelandchemicalindustry.Forinstance,itscompoundsarewidelyappliedintheproceduresofvitrioland petroleumchemicalmanufactureascatalyzers[14].Vanadium hasseveralvalences,butgenerallyitslowvalencesturnintohighoneseasilyinenvironment[5].BecauseV(Ⅴ)isthemost stableandpoisonousone,weoftenlayemphasisonitinenvi ronmentalpollutioncontrol.Vanadiumexistsinenvironmentalwaterwithextremelylowconcentration.Inseawateritscontentislessthan… 相似文献
9.
Synthesis of a new high sensitive water soluble chromogenic reagent meso-tetra-(2-chloro-4-sulfophenyl) prophyrin and the
conditions for the reaction of this reagent with cadmium (I) were studied. The apparent molar absorption coefficient at 434 nm is 5.20×105 L·mol−1·cm−1. The method has been applied to the determination of 5.0×10−9 (content by weight) Cadmium (I) in honey.
Supported by the National Natural Science Foundatyion of China
Xiao Ling: born in Oct. 1962, Ph.D gradute student 相似文献
10.
The thermokinetic reduced extent equations of reversible inhibitions for Michaiels-Menten enzymatic reaction were deduced,
and then the criteria for distinguishing inhibition type was given and the methods for calculating kinetic parameters,K
M,K
i andv
m were suggested. This theory was applied to inverstigate the inhibited thermokinetics of laccase-catalyzed oxidation ofo-dihydroxybenzene bym-dihydroxybenzene. The experimental results show the inhibition belongs to reversible competitive type,K
M=6.224×10−3 mol·L−1,K
i=2.363×10−2 mol·L−1.
Xiong Ya: born Sep. 1961, Ph. D. graduate student. Curent research interest is in biothermochemistry research
Supported by the National Natural Science Foundation of China 相似文献
11.
HeDong-bao LiLi-hua LiQing YangXiao-zhen 《武汉大学学报:自然科学英文版》2004,9(3):371-374
The synergistic interaction between the cationic guar gum (the ammonium hydroxy-propyl-trimethyl chloride of guar gum) and sodium algirmte has been studied. The effects of the mass ratio of them, mixed temperature, balk salt ion concentration, incubation time and pH value on gelation were investigated. It has been observed that there was a gel strength maximum when the mass ratio was 0. 6, the mixed temperature was 70 ~C, the balk salt ion concentration was 1.0 mol ~ L-1 , the incubation time was 30 rain and the pH value was 8. Interaction between molecules of these two polysaccharides was investigated by FT-IR spectrometry. 相似文献
12.
Sensitive Determination of DNA by RLS Enhancement of Metal Ions 总被引:1,自引:0,他引:1
Huang Jian-ping Chen Fang Ai Xin-ping He Zhi-ke . College of Chemistry Molecular Sciences Wuhan University Wuhan Hubei China . Department of Environmental Engineering Hubei Agricultural College Jingzhou Hubei China 《武汉大学学报:自然科学英文版》2004,9(2):234-238
0 IntroductionThequantitativeanalysisofnucleicacids,especiallythemi cro determinationofnucleicacids,isbecomingmoreandmoreimportantinmanybiologicalstudies.Recently ,apromisingspectraltechnique ,whichwasbasedonthemeasurementofen hancedresonancelightscattering (RLS) [1 ,2 ] ,hasgivenrisetostronginterestbyanalystsandbiochemistsfornucleicacidsandproteinassay[3 9] .Uptonow ,manykindscompoundshavebeenfoundRLSenhancementwhilebindingtoDNA ,andallthesecompoundsarecharacterizedofpositivechargewhicha… 相似文献
13.
WANG Juan~ TANG Ping~ ZHAO Fa-qiong~ ZENG Bai-zhao~ . College of Chemistry Molecular Sciences Wuhan University Wuhan Hubei China . Department of Environmental Engineer Jianghan University Wuhan Hubei China 《武汉大学学报:自然科学英文版》2005,10(5):913-918
0Introduction Epinephrine(EP)isanimportantcate cholamineneurotransmitterinthemammaliancentralnervoussystem,whichissecretedbythesu prarenalglandalongwithnorepinephrine.Itisgen erallyusedtotreathypertension,bronchialasthma,organicheartdisease,andincardiacsurgeryand myocardialinfarction[1].VariousmethodshavebeendevelopedforEPstudy,suchascapillaryelectro phoresis(CE)[2],highperformanceliquidchroma tography(HPLC)andflowinjectionanalysis(FIA)[3].Numerousobservationsontheelectro chemicalbehavio… 相似文献
14.
A new method is proposed for the preconcentration of vanadium(V) with crosslinked chitosan (CCTS) and determination by graphite
furnace atomic absorption spectrometry (GFAAS). The adsorption rate of vanadium(V) by CCTS was 97% at pH 4.0, and vanadium(V)
was eluted from crosslinked chitosan with 2 mL 2.0 mol·L−1 chlorhydric acid and determined by GFAAS. The detection limit (3σ,n=7) for vanadium (V) was 4.8×10−12g and the relative standard deviation (R.S.D) at concentration level of 2.6 μg·L−1 is less than 3.6%. The method shows a good selectivity and high sensity, and it was applied to determination of vanadium(V)
in oyster and water samples. The analytic recoveries are (97±5)%.
Biography: Wu Wei (1975-), female, Master candidate, research direction: environmental & analytical chemistry. 相似文献
15.
A coupled chemiluminescence method for the determination of some carboxylic acids was developed, based on their enhancement
the chemiluminescence light emission of the reaction of tirs (2,2′-bipyridine) ruthenium(II) and Ce(IV) in sulfuric acid medium.
The conditions for their determination were optimized. The following detection limits were obtained: oxalic acid, 2.67×10−8 mol/L; propandioic acid, 1.20×10−6 mol/L; pyruvic acid, 1.35×10−8 mol/L; citric acid, 5.10×10−8 mol/L; barbituric acid, 2.48×10−7 mol/L. The proposed method was successfully applied to determination of oxalic acid. The coupled chemiluminescent reaction
mechanism and rate equation are proposed.
Foundation item: Supported by the National Natural Science Foundation of China and the Natural Science Foundation of Hubei
Province
Biography: HAN He-you (1962-), male, Associate professor, phD, graduate candidate 相似文献
16.
ZHENG Qi~ CAO Qihua~ LIU Zhihong~ CAI Ruxiu~ . College of Chemistry Environmental Engineering Jianghan University Wuhan Hubei China . College of Chemistry Molecular Sciences Wuhan University Wuhan Hubei China 《武汉大学学报:自然科学英文版》2006,11(3):677-681
0 IntroductionSltougdiiceasl csoynstceermnsin gharveeac btieveen o txhyeg ei nm psopretcaienst ( oRbjOecSt) iinn bbiioo--medicine and analytical chemistry in recent years[1-4]. Manyi mportant life phenomena and diseases are relatedtotheinter-mediates of ROS[5-7].ROS have beeni mplicated as ani mpor-tant causative factor in cell damage,including apoptosis andnecrosis . Their proposed actions comprise lipid peroxidation,DNAdamage,the mitochondrial respiratory chain destructionand protein mo… 相似文献
17.
Pan Zu-ting Yu Jun-ping College of Chemistry Molecular Sciences Wuhan University Wuhan Hubei China 《武汉大学学报:自然科学英文版》2004,9(3):353-358
0 IntroductionTriamcinoloneAcetonide,9 fluoro 11β,2 1 dihydroxy 16α,17[(1 methylethylidene)bis (oxy) ] pregn 1,4 diene 3,2 0 dione,Mris 4 34.4 8(abr.TA ) .Itbelongstoaclassofadrenalcortexhormonedrug ,whosefunctionsareaffectionofsugarmetabolism ,anti inflammationandresistancehypersusceptibilityetc.TAcanbesuitabletorheumatoidarthritisanddermatosissuchashypersusceptibilityandneuropathicdermatitis .ThefunctionsofTAisstrongerandlongerthantriamcinolone[1 ,2 ] .ThecommonmethodsofdetectingTAm… 相似文献
18.
Cai Ling-shuang Wu Cai-ying Mei Su-rong Zeng Zao-rui College of Chemistry Molecular Sciences Wuhan University Wuhan Hubei China 《武汉大学学报:自然科学英文版》2004,9(3):359-365
0 IntroductionMolecularimprinting[1 ] isatechniquebasedoncreatingcavities,inahighlycross linkedpolymermatrix ,thatcorrespondtothesizeandshapeoftheprintmolecule .Themolecularrecognitioneffectisaconsequenceofthepresenceinthepolymerizationmixtureoftemplatemoleculescapableofestab lishingnon covalentinteractionswithmonomerandcross linkers.Molecularlyimprintedpolymers (MIPs)havebeenusedaschromatographicstationaryphases[2 ,3] includingcapillaryelec trochromatography ,asselectivematerialsforbiosenso… 相似文献
19.
Electrochemical behavior of a carbon paste electrode (CPE) modified with nickel(II)-5, 11, 17, 23-tetra-tert-butyl-25, 27-bis(diethylcarbamoylmethoxy)
calix[4]arene (Ni (II)-L) complex and its electrocatalytic activity towards the oxidation of hydrogen peroxide were investigated
by cyclic voltammetric technique in a 5.0×10−2 mol/L NaClO4+1.0×10−3 mol/L NaOH solution. It was found that Ni(II)-L acts as an effective catalyst for the oxidation of hydrogen peroxide. The
modified electrode exhibited a linear response over a hydrogen peroxide concentrations in the range of 2.0×10−5−1.0×10−4 mol/L with a detection limit as low as 1.0×10−6 mol/L. The relative standard deviation was 3.5% for 5 successive determinations of H2O2 at 1.0×10−5 mol/L. The modified electrode was used successfully in rainwater analysis.
Foundation item: Supported by the Natural Science Foundation of Hubei Province (98J040).
Biography: Li Chun ya(1972-), Ph. D. candidate, research direction: electroanalysis and electrosynthesis. 相似文献
20.
以毛细管区带电泳安培检测法对大鼠血清中班布特罗及其活性代谢物特布他林的水平及药物代谢状况进行了研究.以直径300 μm的碳圆盘电极为工作电极,检测电位为1.10 V (vs. Ag/AgCl),在0.02 mol·L-1磷酸盐缓冲体系中(pH 7.0),当分离电压为10 kV时两种被分析物质在16 min内可基线分离.班布特罗和特布他林浓度分别在2.0×10-5~5.0×10-7mol·L-1和2.0×10-6~5.0×10-8mol·L-1范围内,与峰电流呈良好的线性关系,检测限分别可达2.5×10-7mol·L-1和2.0×10-8mol·L-1.将该法直接用于班布特罗及其活性代谢物特布他林在大鼠血清中的分离与测定,无需样品预处理,分析结果令人满意.该方法具有灵敏度高、简便、易操作等优点,在临床检验和药物动力学研究中具有很好的应用前景. 相似文献