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1.
DU JinFeng LI Ying ZHANG Qi YAO XiaoJun 《科学通报(英文版)》2007,52(2):200-204
The presence of several high affinity binding sites on human serum albumin (HSA) makes it a possible target for many drugs. This study is designed to examine the effect of aloe-emodin on HSA by fluo-rescence, CD spectroscopy and molecular modeling. The results of fluorescence measurements sug-gested that the hydrophobic interaction was the predominant intermolecular force stabilizing the AE-HSA complex, which was in good agreement with the result of molecular modeling study. And the enthalpy change ΔH0 and the entropy change ΔS0 were calculated to be -7.041 kJ·mol-1 and 76.619 J·mol-1·K-1 according to the Van't Hoff equation. The alterations of protein secondary structure in the presence of AE in aqueous solution were quantitatively calculated from CD spectra, and the content of α-helices obviously increased. 相似文献
2.
Flavonols are plant pigments that are ubiquitous in nature. Morin and other related plant flavonols have come into recent
prominence because of their usefulness as anticancer, anti-tumor, anti-AIDS, and other important therapeutic activities of
significant potency and low systemic toxicity. The heat of combustion of morin (molecular formula, C15H10O7·H2O) in oxygen was measured by a rotating-bomb type combustion calorimeter, the standard molar enthalpy of combustion of morin
at T = 298.15 K was determined to be Δc
H
m⦵ (C15H10O7·H2O, s) = −(5 937.99 ± 2.99) kJ · mol−1. The derived standard molar enthalpy of the formation of morin in solid powder state at T = 298.15 K, Δf
H
m⦵ (C15H10O7·H2O, s), was −(1 682.12 ± 3.58) kJ · mol−1, which provide an accurate data of the stability of morin to the pharmacy and pharmacology.
Biography: HOU Hanna(1956–), female, Visiting researcher, Associate professor of Hubei University of Education, research direction:
thermochemistry. 相似文献
3.
Zikun Wang 《科学通报(英文版)》1999,44(5):418-418
The definition of n-parameter infinite-dimensional (r,δ) -Ornstein-Uhlenbeck process {xt (·) } ((r, δ)-OUPn∞ for short) is given. The absolute continuity of distribution μt of Xt(·) and the limit of Xt(·) when |t| →∞ is discussed. 相似文献
4.
Wang Tianzhi Li Weiping Wu Dingquan Wan Hongwen Yang Feng Qu Songsheng 《武汉大学学报:自然科学英文版》1997,2(4):511-513
The reaction between Laccase ando-methoxyphenol have been studied by LKB-2107 batch microcalorimetry system. Thermodynamic parameters Δ
r
H
m
, ΔG
o
, ΔG
T
≠
and kinetic parameters (K
m
,k
2) have been determined. The process of the reaction has been analyzed from changes in energy by using the transition state
theory. Two methods for enhancing catalytic power of Laccase are proposed. The results shown that formation of an enzyme-substrate
complex is “anticatalytic”. The enter and sole source of catalytic power is the stabilization of transition state; reactant-state
interactions are by nature inhibitory and only waste catalytic power.
Supported by the National Natural Science Foundation of China
Wang Tianzhi: born in 1968. Ph D 相似文献
5.
Directional solidification and physical properties measurements of the zinc-aluminum eutectic alloy 下载免费PDF全文
Zn-5wt% Al eutectic alloy was directionally solidified with different growth rates (5.32–250.0 μm/s) at a constant temperature gradient of 8.50 K/mm using a Bridgman-type growth apparatus. The values of eutectic spacing were measured from transverse sections of the samples. The dependences of the eutectic spacing and undercooling on growth rate are determined as λ=9.21V-0.53 and ΔT=0.0245V0.53, respectively. The results obtained in this work were compared with the Jackson-Hunt eutectic theory and the similar experimental results in the literature. Microhardness of directionally solidified samples was also measured by using a microhardness test device. The dependency of the microhardness on growth rate is found as Hv=115.64V0.13. Afterwards, the electrical resistivity (r) of the casting alloy changes from 40×10-9 to 108×10-9 Ω·m with the temperature rising in the range of 300–630 K. The enthalpy of fusion (ΔH) and specific heat (Cp) for the Zn-Al eutectic alloy are calculated to be 113.37 J/g and 0.309 J/(g·K), respectively by means of differential scanning calorimetry (DSC) from heating trace during the transformation from liquid to solid. 相似文献
6.
Kinetic studies of the decomposition reaction of dinuclear Fe(Ⅱ) adducts [Fe2(N-Et-HPTB){O2P(OPh)2}](Cl- O4)2 (1) and [Fe2(N-Et-HPTB) {O2P(Ph)2}] (ClO4)2 (2) with O2 have been carried out at low temperature using UV-vis spectra. The decomposition reaction of Fe(Ⅱ)/O2 adducts was first-order in the experimental conditions, and the activation parameters were obtained. ?H¹ = 85.62 kJ·mol-1, ?S¹ = 19.43 J·mol-1·K-1 for compound (1) and ?H¹ = 97.97 kJ·mol-1, ?S¹ = 55.68 J·mol-1·K-1 for compound (2). These results are similar to those of dioxygen adducts of other metals complexes and natural enzymes such as methane mono- oxygenase (MMOH). 相似文献
7.
Fe+ ion beams with the energy of 110 keV were implanted into films of L(+)-cysteine (HSCH2CH(NH2)COOH). One of the single crystals grown in hydrochloric acid solution with the implanted samples through slow evaporation
was structurally characterized by the X-ray crystallography. The crystal is monoclinic, space group C2, with a = 1.8534(4) nm, b = 0.5234(1) nm, c = 0.7212(1) nm, β= 103.72°, V = 0.67965(3) nm3, Z = 4, F(000) = 144.0, D{clac} = 1.763 g · cm−3, μ(MoK
a = 1.06 mm−1, T = 293(2) K. R = 0.0379, wR = 0.0835 for 660 observed reflections (I > 2σ(I)). The structural formula of the crystal compound is (CH2CH(NH2)NO2)ClFe (M
r = 180.38 u). Products of heavy ion beam irradiation were purified and it was directly confirmed that the implanted Fe+ ions had been deposited in the novel molecules. The same doses of Fe+ ion beams of the same energy were implanted into films of L(+)-cysteine hydrochloride monohydrate. FTIR spectroscopy of the implanted samples proved that some of the original molecules
were seriously damaged and significant modifications were induced. 相似文献
8.
14C measurement of forest soils in Dinghushan Biosphere Reserve 总被引:2,自引:0,他引:2
Shen Chengde Liu Dongsheng Peng Shaolin Sun Yanmin Jiang Mantao Yi Weixi Xing Changping Gao Quanzhou Li Zhi’an Zhou Guoyi 《科学通报(英文版)》1999,44(3):251-251
Organic carbon in forest soils of Qingyunsi and Wukesong profiles can be divided into fast and slow components. Δ 14C values of these profiles decrease with increasing of depth. The Δ 14C values in 30-40 cm depth interval of Wukesong profile are decreasing sharply until a very low value, showing that a strong geological environment change occurred about 1 560 years ago. The 14C apparent ages of Wukesong profile show that the coniferous and broad-leaf mixed forests around Wukesong profile have been developing since 425 a BP, which is consistent with historical documents. The penetrating depths of "bomb 14C" in Qingyunsi and Wukesong profiles are 10 and 20 cm, respectively. 相似文献
9.
The characterization of the algaeAnabaena cylindrica solution with Fe (III) was investigated using fluorescence emission and synchronous-scan spectroscopy. The ranges of concentrations
of algae and Fe (III) in aqueous solutions were 5.0×108 2.5×108 cell/L and 1060 μmol/L. respectively. The effective characterization method used was synchronous-scan fluorescence spectroscopy
(SFS). The wavelength difference (Δλ) of 90 nm, was maintained between excitation wavelength (λen) and emission wavelength
(λen). The peak was observed at about (λex) (λem) 326 nm for synchronous scan fluorescence spectroscopy. The fluorescence
quenching in system of algae Fe(III)-HA was studied using synchronous-scan spectroscopy for the first time, Fe(III) was clearly
the effective quencher. The relationship betweenI I (quenching efficiency) andc (concentration of Fe (III) added) was, a linear correlation for the algae solution with Fe(III). Also, Aldrich humie acid
(HA) was found to be an effective quencher.
Foundation item: Supported by the National Natural Science Foundation of China (20177017) and the Scientific Research Foundation of Wuhan
Environmental Protection Bureau
Biography: LIU Xian li(1965), male. Ph.D. Associate professor, research direction: environmental chemistry. 相似文献
10.
Predicting toxicity of aromatic ternary mixtures to algae 总被引:1,自引:0,他引:1
11.
The Na^+/H^+ antiporter plays key roles in maintaining low cytoplasmic NaNa^+ level and pH homeostasis, while little is known about the Carboxyl-terminal hydrophilic tails of prokaryotic antiporters. In our previous study, the first Na^+/H^+ antiporter gene nhaH from moderate halophiles was cloned from Halobacillus dabanensis D-8 by functional complementation. A topological model suggested that only nine amino acid residues (^395PLIKKLGMI403) existed in the hydrophilic C-terminal domain of NhaH. The C-terminal truncated mutant of NhaH was constructed by PCR strategy and designated as nhaH△C. Salt tolerance experiment demonstrated that the deletion of hydrophilic C-terminal nine amino acid residues significantly inhibited the complementation ability of E. coil KNabc, in which three main Na^+/H^+ antiporters nhaA, nhaB and chaA were deleted. Everted membrane vesicles prepared from E. coil KNabc/nhaHAC decreased both Na^+/H^+ and Li^+/H^+ exchange activities of NhaH, and also resulted in an acidic shift of its pH profile for Na^+, indicating a critical role of the short C-terminal domain of NhaH antiporter in alkali cation binding/translocation and pH sensing. 相似文献
12.
Rezaei G. Behbehani 《科学通报(英文版)》2009,54(6):1037-1042
The interaction of Cu2+ with the first 16 residues of the Alzheimer's amyliod β peptide, Aβ (1-16), was studied by employing isothermal titration calorimetry at pH 7.2 and 37℃ in aqueous solution. The Gholamreza Rezaei Behbehani (GRB) solvation model was used to reproduce the enthalpies of Cu2++ Aβ(1-16) interaction over the whole Cu2+ concentrations. The binding parameters recovered from the solvation model were attributed to the structural change of Aβ (1-16) due to the metal ion interaction. It was found that there is a set of two identical and non interacting binding sites for Cu2+ ions. The molar enthalpy of binding is ΔH=27.895 kJ/mol. The association binding constants are 1.895 μm-1 and 1.891 μm-1 for the first and second binding sites respectively. 相似文献
13.
Phenotypic analysis of the medullary-type CD4 CD8+ (CD8SP) thymocytes has revealed phenotypic heterogeneity within this cell population. The phenotype of mature peripheral
CDS+T cells is TCRαβ+CD3+Qa-2+HSA 3G11−6C10, whereas in the medullary-type CD8SP thymocytes, 20% are Qa-2+; 33%, HAS; 30%, 3G11; and 70% are 6C10. The disparate expression patterns of these four cell surface markers suggest that
medullary-type CD8SP thymocytes may undergo phenotypic maturation process. According to the distribution of these four cell
surface markers, six subgroups of CD8SP thymocytes have been identified. The precursor-progeny relationship along with developmental
pathway is postulated as follows: 6C10+HSA+3G11 Qa-2→ 6C10+HSA+ 3G11+Qa-2 → 6C10 HSA+3G11+Qa-2 → 6C10−HSA−3G11+Qa-2 → 6C10−HSA−3G11 Qa-2 → 6C10−HA S 3G11 Qa-2+, the cells in the last subgroup exit the thymus and home into periphery. 相似文献
14.
15.
A coupled chemiluminescence method for the determination of some carboxylic acids was developed, based on their enhancement
the chemiluminescence light emission of the reaction of tirs (2,2′-bipyridine) ruthenium(II) and Ce(IV) in sulfuric acid medium.
The conditions for their determination were optimized. The following detection limits were obtained: oxalic acid, 2.67×10−8 mol/L; propandioic acid, 1.20×10−6 mol/L; pyruvic acid, 1.35×10−8 mol/L; citric acid, 5.10×10−8 mol/L; barbituric acid, 2.48×10−7 mol/L. The proposed method was successfully applied to determination of oxalic acid. The coupled chemiluminescent reaction
mechanism and rate equation are proposed.
Foundation item: Supported by the National Natural Science Foundation of China and the Natural Science Foundation of Hubei
Province
Biography: HAN He-you (1962-), male, Associate professor, phD, graduate candidate 相似文献
16.
Li Li-qing Chen Dong-hua College of Chemistry Life Sciences South Central University for Nationalities Wuhan Hubei China 《武汉大学学报:自然科学英文版》2004,9(2):239-246
0 IntroductionThetechnologyofthermalanalysishasbeenwidelyusedinthestudyofthermalbehaviorandthermalcharacterofsolidstatereaction ,theprimaryintentionofthermalanalysiskineticsistoestablishseparatevaluesofapparentactivationenergyE ,themostprobablemechanism g(α)andthepre exponentialfactorA ,meanwhilethedeterminationofg(α)hasgraduallybeenthefocusofthisfield .Inthisfield ,therearemostlygeneralkineticsmethodsused presently ,suchasCoats Redfern’s[1 ] integralmethod ,Achar’s[2 \〗differentialmet… 相似文献
17.
Hong Liang Bengang Xin Xiujian Wang Yuzhou Yuan Yongqia Zhou Panwen Shen 《科学通报(英文版)》1998,43(5):404-409
The interaction of Cu(II) and human serum albumin (HSA) or bovine serum albumin (BSA) at physiological pH is studied by equilibrium
dialysis. The successive stability constants are obtained by non-linear least square methods fitting Bjerrum formula. For
both the Cu(II)-HSA and Cu(II)-BSA systems, the order of magnitude of K1 and K2 was found to be ≈104 mol−1· dm3. There are about twenty stoichiometry binding sites found in one HSA or BSA molecule. They can be divided into two or three
sets. Results of equilibrium dialysis experiments suggest that there exists one strong metal binding site in both Cu(II)-HSA
and Cu (II)-BSA. It is the imidazol group nitrogen atoms of His3 that are primarily concerned with copper binding site. After reaching dialysis equilibrium, there is the interaction among
the different binding sites, the values ofK all deviate from the simple statistical effect except forK
1 and K2 in both Cu(II)-HSA and Cu(II)-BSA systems, and the positive cooperative effect is found. 相似文献
18.
A novel material MnO2 nanosheet has been used as the support matrix for the immobilization of horseradish peroxidase (HRP). HRP entrapped in MnO2 nanosheet film exhibits facile direct electron transfer with the electron transfer rate constant of 6.86 s^-1. The HRP/MnO2 nanosheet film gives a reversible redox couple with the apparent formal peak potential (E^0') of -0.315 V (vs. Ag/AgCl) in pH 6.5 phosphate buffer solution (PBS). The formal potential E^0' of HRP shifts linearly with pH with a slope of -53.75 mV.pH^-1, denoting that an electron transfer accompanies single-proton transportation. The immobilized HRP shows an electrocatslytic activity to the reduction of H2O2. The response time of the biosensor for H2O2 is less than 3 s, and the detection limit is 0.21 μmol · L^-1 based on signal/noise = 3. 相似文献
19.
QIN Yimin PAN Nina ZOU GuolinCollege of Life Sciences Wuhan University Wuhan Hubei China 《武汉大学学报:自然科学英文版》2006,11(2):432-436
0Introduction Porphyrinderivativeshavebeenusedasphotosensitizersinphotodynamictherapy(PDT),anewapproachdevel opedforthetreatmentofcancer[1].Cationwater solublepor phyrinsareofconsiderableinterestowingtotheirpossiblebio medicalapplications[2].Inwhich,theinhibiteffectonthe growthmetabolismofE.coliandcancercellHL60ofmeso tetra(4N ethylacetatepyridyl)porphyrin(H2NEAE pp)and itsZn(Ⅱ)complexform(ZnNEAE pp)havebeenrecentlyin vestigated[3,4].Toenhancetumor locationandanticancerac tivitiesofdr… 相似文献
20.
Meso-tetrahydroxylphenyl chlorin (m-THPC) is one of the most efficient prospective sensitizers used in photodynamic therapy (PDT). ESR spectroscopy, fluorescence quenching experiments and cyclic voltammogram measurement were used to study its redox properties. The results showed that the ability of m-THPC generating superoxide radical anions was very strong, and the rate constant of m-THPC fluorescence quenching by oxygen kq (O2)=1.46×1010 mol-1·s-1. The values of fluorescence quen- ching rate constant of m-THPC by some other electron acceptors, such as methyl viologen (MV2+) and anthraquinone (An), were also measured. And they were kq (MV2+)=5.51×109 mol-1·s-1, kq (An)=7.81×109 mol-1·s-1. The oxidation potential of m-THPC was examined to be +0.62 V (vs. NHE) in acetonitrile. All these suggested that m-THPC should be a much stronger electron donor than photofrin, the currently used in clinical photodrug, and may react easily through electron transfer with biological matter to yield various radicals. So it seemed reasonable that the type Ⅰ reaction may play an important role in the high activity of m-THPC-PDT. 相似文献