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1.
The electroanalytical method of ferriheme was studied by linear sweep voltammetry in medium of 0.05 mol/L Tris+0.05 mol/L
NH3−NH4Cl buffer at hang mercury drop electrodes (HMDE). Heme exhibits two pair reversible redox peaks and one irreversible peak.
The cathodic peak potentials are−;0.236 V, −0.422 V and −1.408 V respectively. The first and the third peaks can be used for
directly quantitative determination of heme concentrations. The peak currents are good linear relationship with heme concentration
in ranges of 3×10−6–6×10−5 mol/L and 3×10−7–1.5×10−5 mol/L respectively.
Supported by the National Natural Science Foundation of China
Lju Huihong: born in 1965, Lecturer. Visiting scholar from Xiangyang Teacher’s College, Hubei, 441053 相似文献
2.
A series of experimental methods including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) test, alkaline
phosphatase (ALP) activity measurement, mineralized function, Oil Red O stain and measurement were employed to assess the
effect of Dy3+ on the osteogenic and adipogenic differentiation of mouse primary bone marrow stromal cells (BMSCs) and the adipogenic trans-differentiation
of mouse primary osteoblasts (OBs). The results showed that Dy3+ had no effect on BMSC proliferation at concentrations of 1×10−8 and 1×10−5 mol/L, but inhibited BMSC proliferation at other concentrations. Dy3+ had no effect on OB proliferation at concentrations of 1×10−10 and 1×10−9 mol/L, but inhibited OB proliferation at other concentrations. Dy3+ had no effect on the osteogenic differentiation of BMSCs at concentrations of 1×10−9 and 1×10−7 mol/L, and promoted osteogenic differentiation of BMSCs at other concentrations at the 7th day. The osteogenic differentiation
of BMSCs was inhibited by Dy3+ at concentration of 1×10−5 mol/L at the 14th day, but promoted osteogenic differentiation of BMSCs at concentrations of 1×10−9, 1×10−8, 1×10−7 and 1×10−6 mol/L with the maximal effect at concentration of 10−6 mol/L. Dy3+ promoted mineralized function of BMSCs at any concentration. Dy3+ had no effect on adipogenic differentiation of BMSCs at concentration of 1×10−7 mol/L, but inhibited adipogenic differentiation of BMSCs at other concentrations. Dy3+ inhibited adipocytic trans-differentiation of OBs at any concentration, suggesting that Dy3+ had protective effect on bone and the protective effect on bone may be mediated by modulating differentiation of BMSCs away
from the adipocyte and inhibiting adipocytic trans-differentiation of OBs which may promote differentiation and mineralization
of OBs. These results may be valuable for better understanding the mechanism of the effect of Dy3+ on pathogenesis of osteoporosis.
Supported by the Foundation for Key Program of Ministry of Education of China (Grant No. 208018) 相似文献
3.
Determination of isocarbophos by cathodically sweeping oscilliopolarography is described. In a 1.0×10−5 mol/L sodium dodecylbenzene sulfonate (SDS)+0.1 mol/L HAC-NaAC (pH=4.0) buffer medium, isocarbophos and its alkaline hydrolysate
exhibited sensitive second derivative wave at −0.50 V and −0.48 V respectively. The peak current was linearly proportional
to the concentration of isocarbophos in the range of 5.40×10−6−1.05×10−4 mol/L by detecting isocarbophos directly. The detection limit was 3.60×10−6 mol/L with the relative standard derivation (RSD) of 7.3%. By employing an alkaline hydrolysis, the peak current was linearly
proportional to the concentration of isocarbophos in the range from 7.70×10−7 mol/L to 1.24×10−4 mol/L, and the detection limit was 5.80×10−7 mol/L with RSD of 3.1%. The hydrolysis procedure and the electrode reaction were studied by voltammetry.
Foundation item: Supported by the Foundation of Chinese-France Cooperation Program on Advanced Research
Biography: ZHANG Wu-ming (1929-), female, Professor. 相似文献
4.
A new chemiluminescence (CL) method for the determination of tetracycline and oxytetracycline is developed, based on the CL
reaction of tetracycline and oxytetracycline with Ru (bipy)3
2+ and Ce (IV). In sulfuric acid medium, the CL emission is generated upon continuous oxidtion of Ru(bipy)3
2+ by cerium (IV). The emission intensity is greatly enhanced when tetracycline and oxytetracycline are introduced into the
reaction system after acid degradation. Under the optimum conditions, the calibration curves are linear over the range of
8.0×10−8∼4.0×10−6 mol/L for tetracycline and of 2.0×10−7∼4.0×10−5 mol/L for oxytetracycline, with the detection limits are 4.2×10−8 mol/L for tetracycline and 1.5×10−7 mol/L for oxytetracycline, respectively. The proposed method was used for the determination of tetracycline and oxytetracycline
in pharmaceutical formulations with good results.
Foundation item: Supported by the National Natural Science Foundation of China and the Natural Science Foundation of Hubei
Province.
Biography: HAH He-you (1962), male, Associate profeddor, Ph.D graduate candidate. Present address, Department of Chemistry,
Huainan Teacher's College, Huainan. 相似文献
5.
A novel spectrofluorimetric method for the determination of L-ascorbic acid is proposed. It is based on the inhibition of
L-ascorbic acid on the formation of 2,3-diaminophenazine, which is an oxidation product ofo-phenylenediamine catalyzed by laccase. The fluorescence (at λcx/λcm=464 nm /530 nnm) was enhanced strongly in the presence of organic media. The mechanism ofo-phenylenediamine oxidation reaction catalyzed by laccase in the presence of L-ascorbic acid is discussed. L-ascorbic acid
is determined in the ethanol, 1,4-dioxane and acetone over the linear range of 4.0×10−7≈1.2×10−4 mol/L, 4.0×10−7≈8.0×10−5 mol/L and 4.0×10−7≈1.0×10−4 mol/L with a detection limit of 1.20×10−5 mol/L, 1.19×10−5 mol/L and 1.24×10−8 mol/L, respectively. The method has been successfully applied to the simple and rapid determination of L-ascorbic acid in
pharmaceuticals and milk powder.
Supported by National Natural Science Foundation of China and the Special Funds of State Education Committe for Doctorate
Research
Huang Zuyun: born in Aug. 1963. Ph.D 相似文献
6.
A coupled chemiluminescence method for the determination of some carboxylic acids was developed, based on their enhancement
the chemiluminescence light emission of the reaction of tirs (2,2′-bipyridine) ruthenium(II) and Ce(IV) in sulfuric acid medium.
The conditions for their determination were optimized. The following detection limits were obtained: oxalic acid, 2.67×10−8 mol/L; propandioic acid, 1.20×10−6 mol/L; pyruvic acid, 1.35×10−8 mol/L; citric acid, 5.10×10−8 mol/L; barbituric acid, 2.48×10−7 mol/L. The proposed method was successfully applied to determination of oxalic acid. The coupled chemiluminescent reaction
mechanism and rate equation are proposed.
Foundation item: Supported by the National Natural Science Foundation of China and the Natural Science Foundation of Hubei
Province
Biography: HAN He-you (1962-), male, Associate professor, phD, graduate candidate 相似文献
7.
A second derivative polarographic wave of naphthalene is observed in 50% N, N-dimethylformamide and ammonia-ammonium chloride
medium. The peak potential is about −1.05 V (vs. SCE). The concentration of naphthalene is linear with peak current in the
range of 5.00×10−6 mol/L to 5.00×10−4 mol/L, The detection limit is 2.00×10−6 mol/L. This method can be used to determine naphthalene content of the sample such as coal, mothball and asphaltic bitumen.
The relative standard deviation (RSD) are in the range of 0.38%≈1.30%. The determined recoveries by adding known amounts of
naphthalene to sample solution are in the range of 92%≈101%. This paper also study on the polarographic behavior of naphthalene.
Biography: ZHANG Li-hua (1974-), female, Master. 相似文献
8.
The chemiluminescence (CL) of luminol-Cu (II) was applied to HPLC determination of ascorbic acid, which was separated by a
C18 reverse-phase column with a mobile phase of 0.25 mol/L HAc. The eluted ascorbic acid was mixed with 0.3 mmol/L luminol and
0.05 mol/L CuSO4. The light emission from the reaction of Cu(II) oxidized ascorbic acid and luminol was detected by a modified luminometer.
The detection limit was 3.6×10−6 mol/L for ascorbic acid at aS/N ratio of 3, and the linear calibration range was 2×10−4–2×10−3 mol/L. The relative standard deviation for 5 replicate injections of 1×10−3 mol/L ascorbic acid was calculated as 4.3%. The method was successfully applied to determination of ascorbic acid in juice
beverage.
Foundation item: National Natural Science Foundation of China (29605001)
Biography: Wu Feng-wu(1963-), male, research direction: analytical chemistry. 相似文献
9.
Electrochemical behavior of a carbon paste electrode (CPE) modified with nickel(II)-5, 11, 17, 23-tetra-tert-butyl-25, 27-bis(diethylcarbamoylmethoxy)
calix[4]arene (Ni (II)-L) complex and its electrocatalytic activity towards the oxidation of hydrogen peroxide were investigated
by cyclic voltammetric technique in a 5.0×10−2 mol/L NaClO4+1.0×10−3 mol/L NaOH solution. It was found that Ni(II)-L acts as an effective catalyst for the oxidation of hydrogen peroxide. The
modified electrode exhibited a linear response over a hydrogen peroxide concentrations in the range of 2.0×10−5−1.0×10−4 mol/L with a detection limit as low as 1.0×10−6 mol/L. The relative standard deviation was 3.5% for 5 successive determinations of H2O2 at 1.0×10−5 mol/L. The modified electrode was used successfully in rainwater analysis.
Foundation item: Supported by the Natural Science Foundation of Hubei Province (98J040).
Biography: Li Chun ya(1972-), Ph. D. candidate, research direction: electroanalysis and electrosynthesis. 相似文献
10.
Pan Zu-ting Yu Jun-ping College of Chemistry Molecular Sciences Wuhan University Wuhan Hubei China 《武汉大学学报:自然科学英文版》2004,9(3):353-358
0 IntroductionTriamcinoloneAcetonide,9 fluoro 11β,2 1 dihydroxy 16α,17[(1 methylethylidene)bis (oxy) ] pregn 1,4 diene 3,2 0 dione,Mris 4 34.4 8(abr.TA ) .Itbelongstoaclassofadrenalcortexhormonedrug ,whosefunctionsareaffectionofsugarmetabolism ,anti inflammationandresistancehypersusceptibilityetc.TAcanbesuitabletorheumatoidarthritisanddermatosissuchashypersusceptibilityandneuropathicdermatitis .ThefunctionsofTAisstrongerandlongerthantriamcinolone[1 ,2 ] .ThecommonmethodsofdetectingTAm… 相似文献
11.
WANG Juan~ TANG Ping~ ZHAO Fa-qiong~ ZENG Bai-zhao~ . College of Chemistry Molecular Sciences Wuhan University Wuhan Hubei China . Department of Environmental Engineer Jianghan University Wuhan Hubei China 《武汉大学学报:自然科学英文版》2005,10(5):913-918
0Introduction Epinephrine(EP)isanimportantcate cholamineneurotransmitterinthemammaliancentralnervoussystem,whichissecretedbythesu prarenalglandalongwithnorepinephrine.Itisgen erallyusedtotreathypertension,bronchialasthma,organicheartdisease,andincardiacsurgeryand myocardialinfarction[1].VariousmethodshavebeendevelopedforEPstudy,suchascapillaryelectro phoresis(CE)[2],highperformanceliquidchroma tography(HPLC)andflowinjectionanalysis(FIA)[3].Numerousobservationsontheelectro chemicalbehavio… 相似文献
12.
The detection of human fibrin has been performed by immunological piezoelectric quartz crystal microbalance technique. Anti-fibrin
antibody was immobilized onto the gold electrodes of a 9 MHz AT-cut piezoelectric quartz crystal with bovine serum albumin
adsorption, followed by glutaraldehyde cross-linking. The antibody-bound crystals had good response to human fibrin in the
concentration range of 10−4∼10−1g/L. The sensor system had good selectivity. The crystal could be regenerated nearly 10 times without detectable decrease
of sensitivity.
Supported by The National Natural Science Foundation of China
Pei Renjun: born in Nov. 1971, Ph. D. graduate student 相似文献
13.
Wu Feng-wu Ai Xin-ping Yan Chen-nong He Zhi-ke Meng Hui Zeng Yun-e 《武汉大学学报:自然科学英文版》2002,7(3):341-344
The light emission produced by thiourea in oxidation process by permanganate in acidic solution in the presence of Ru(phen)
2
3
+ is used to determine 1.0×10−5 −1.0×10−5 mol/L thiourea. The limit of detection is 9.8×10−9 mol/L and the relative standard deviation is 1.1% for a 1.0×10−5 mol/L thiourea solution (n=10). The method was applied satisfactorily to the determination of thiourea.
Foundation item, Support by Ministry of Education, Wuhan University and Alumni Association of it
Biography: Wu Feng-wu(1963-), Male, Ph. D, research direction: analytical chemistry. 相似文献
14.
SHI Bo’an~ ZENG Danyun~ JI Xinghu~ XI Juan~ AI Xinping~ HE Zhike~ . College of Chemistry Molecular Sciences Wuhan University Wuhan Hubei China . Department of Chemistry Chemical Engineering Hubei Institute for Nationalities Enshi Hubei China 《武汉大学学报:自然科学英文版》2006,11(3):672-676
0 IntroductionThbiep ycroidmipnlee)xru ctahteinoinu mof(Ⅱ r)u t(he Rniuu(mbip,y m)3ai2n +ly) atrnids- t(r2is -,(21’ -,10-phenanthroline)ruthenium(Ⅱ) (Ru(phen)32 +) ,is a kindof sensitive analytical reagent for electrogenerated chemilumi-nescence(ECL) and chemiluminescence(CL) ,on which a par-ticular review has been presented[1]. The earliest publicationon the synthesis of Ru(bipy)32 +appeared in 1936[2], andduringthefollowing30 years ,theinvestigations relatedtothissubstance were only in… 相似文献
15.
LUO Jia HUANG YanYan XIONG ShaoXiang LIU GuoQuan ZHAO Ruit 《科学通报(英文版)》2007,52(10):1311-1319
The specific interaction between sense and antisense peptides was studied by high-performance affinity chromatography (HPAC) and quartz crystal microbalance (QCM) biosensor. Fragment 1-14 of human interferon-β (hlFN-βwas chosen as sense peptide and its three antisense peptides (AS-IFN 1, AS-IFN 2, and AS-IFN 3) were designed according to the degeneracy of genetic codes. The affinity column was prepared with sense peptide as ligand and the affinity chromatographic behavior was evaluated. Glu-substituted antisense peptide (AS-IFN 3) showed the strongest binding to immobilized sense peptide at pH 7.5. A quartz crystal microbalance-flow injection analysis (QCM-FIA) system was introduced to investigate the recognition process in real-time. The equilibrium dissociation constants between sense peptide and AS-IFN 1, AS-IFN 2 and AS-IFN 3 measured 2.08×10^-4, 1.31×10^-4 and 2.22×10^-5 mol/L, respectively. The mechanism study indicated that the specific recognition between sense peptide and AS-IFN 3 was due to sequence-dependent and multi-modal affinity interaction. 相似文献
16.
Crystal structure and negative thermal expansion of solid solution Lu<Subscript>2</Subscript>W<Subscript>3−<Emphasis Type="Italic">x</Emphasis></Subscript>Mo<Subscript><Emphasis Type="Italic">x</Emphasis></Subscript>O<Subscript>12</Subscript>
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Jie Peng Xin-zhi Liu Fu-li Guo Song-bai Han Yun-tao Liu Dong-feng Chen Zhong-bo Hu 《矿物冶金与材料学报》2010,17(6):786-790
A new series of solid solutions Lu2W3−x
Mo
x
O12 (0.5≤x≤2.5) were successfully synthesized by the solid-state method. Their crystal structure and negative thermal expansion properties
were studied using high-temperature X-ray powder diffraction and the Rietveld method. All samples of rare-earth tungstates
and molybdates are found to crystallize in the same orthorhombic structure with space group Pnca and show the negative thermal expansion phenomena related to transverse vibration of bridging oxygen atoms in the structure.
Thermal expansion coefficients (TEC) of Lu2W3−x
Mo
x
O12 are determined as −20.0×10−6 K−1 for x=0.5 and −16.1×10−6 K−1 for x=2.5 but -18.6×10−6 and −16.9×10−6 K−1 for unsubstituted Lu2W3O12 and Lu2Mo3O12 in the identical temperature range of 200 to 800°C. High-temperature X-ray diffraction (XRD) data and bond length analysis
suggest that the difference between W-O and Mo-O bond is responsible for the change of TECs after the element substitution
in this series of solid solutions. 相似文献
17.
A new method of determination of trace CN− in water was proposed. A thin film of Ag2S was electrochemically deposited on both sides of a silver coated piezoelectric crystal. The Ag2S−Ag films can react with CN− selectively, which causes the frequency shifts of the plezoelectric crystal. The frequency shifts are proportional to the
concentration of CN− in the range of 10−6≈10−3 g/L. XPS experiments indicated the sensing film was Ag2S−Ag. A coordination complex Ag−CN− was formed when Ag2S−Ag film contacted with solution containing CN− ion. A response mechanism was proposed based on the XPS data.
Supported by National Natural Science Foundation of China
Hu Jiming: born in Sept, 1952, Professor 相似文献
18.
Determination of omethoate by cathodically sweeping oscillopolarography is described. The product of the alkaline hydrolysis
exhibited a sensitive second derivative wave at −0.50 V (vs. SCE) in a 1.0 ×10−5mol/L sodium dodecylbenzene sulfonate (SDBS)+0.1 mol/L HAc-NaAc (pH 4.0) buffer. The peak current was linearly proportional
to the concentration of omethoate in the range from 6.4×10−7 to 5.1×10−5 mol/L. The detection limit is 2.0×10−7 mol/L with the relative standard derivation (RSD) of 3.5%. The hydrolysis procedure and the electrode reaction were studied
by voltammetry.
Supported by the Foundation of Chinese-Franch Cooperation Programme on the Advanced Research
Wang Yu: born in 1964. Lecturer. Cangzhou Teacher’s College, Cangzhou, Hebei, 061001 相似文献
19.
ZHOU Lei TIAN Jiwei ZHANG Xiaoqian 《科学通报(英文版)》2005,50(24):2885-2890
The turbulent dissipation along with temperature and salinity was measured on board R/V Dong Fang Hong 2 over the shelf break of the East China Sea from March 7 to 9, 2004. Applying the Turner angle, it is found that the 'diffusive' double diffusion, salt-fingering, and stable stratification coexist in the upper water column of about 50 m. Below that depth, there exists weak salt-fingering. The turbulent dissipation rates are enhanced along the ray paths of M2 internal tides emanating from the shelf break with values ranging from 1.0×10^-9 W·kg^-1 to 1.2×10^-6 W·kg^-1. The corresponding diapycnal mixing rates are from 1×10^-6 m^2· s^-1 to 1×10^-2 m^2· s^-1. The spatially averaged mixing rate over the whole observation section is 2.3×10^-3 m^2· s^-1, which is much larger than 1×10^-5 m^2·s^-1 of the background diapycnal mixing rate in the open ocean. 相似文献
20.
The state of Tb3+ is investigated in liposome. When the concentration of PC is below CMC (critical micell concentration), most of Tb3+ is associated with PC, the binding constant is about 3.35×103 L/mol. When the concentration of PC is beyond CMC, most of Tb3+ is dimerized, the dimerization constant is about 3.92×104L/mol. In PC−CH−H2O system, the binding constant of Tb3+−CH complex 2.93×104L/mol is obtained.
Biography:Mu Xiao-jing (1974-), female, MS. 相似文献