共查询到20条相似文献,搜索用时 484 毫秒
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The thyroid-hormone receptor can, in the absence of its ligand, suppress activity of a responsive promoter. Addition of thyroid hormone, however, results in the stimulation of expression. The oncogenic derivative of the thyroid-hormone receptor, v-erbA, acts as a constitutive repressor and, when coexpressed with the receptor, blocks activation by thyroid hormone. Thus, v-erbA may be the first example of a dominant negative oncogene. 相似文献
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Spatial and temporal expression of the retinoic acid receptor in the regenerating amphibian limb 总被引:10,自引:0,他引:10
Retinoic acid is known to have dramatic effects on vertebrate limb pattern in development and regeneration, supporting a model in which a gradient of retinoic acid serves as a morphogen to differentially supply positional information to a developing limb. The discovery of a retinoic acid receptor (RAR) and its homology to the steroid and thyroid hormone receptors provided a potential molecular mechanism for limb morphogenesis. One prediction of this model is that the receptor must be expressed in the developing and regenerating limb anlage. We investigated the expression of the RAR in the adult newt, Notophthalmus viridescens, whose amputated limbs are capable of regenerating and upon which retinoic acid can act to alter pattern. We report the cloning of cDNAs encoding a functional newt RAR and the localization of high and uniform levels of RAR mRNA specifically in the regenerating cells that control limb pattern. These results indicate that the morphogenic field is established through differential activation of pre-existing retinoic acid receptors rather than differential expression of the RAR gene. 相似文献
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A novel steroid thyroid hormone receptor-related gene inappropriately expressed in human hepatocellular carcinoma 总被引:7,自引:0,他引:7
We have previously isolated from a human hepatocellular carcinoma a hepatitis B virus integration in a 147-base-pair cellular DNA fragment, similar to steroid- and c-erb-A/thyroid-hormone receptor genes. We have now cloned the corresponding complementary DNA from a human-liver cDNA library. Nucleotide sequence analysis revealed that the overall structure of the cellular gene, which we have named hap, is similar to that of the DNA-binding hormone receptors. That is, it displays two highly conserved regions identified as the putative DNA-binding and hormone-binding domains of the c-erb A/steroid receptors. Six out of seven hepatoma and hepatoma-derived cell-lines express a 2.5-kilobase (kb) hap messenger RNA species which is undetectable in normal adult and fetal livers but present in all non-hepatic tissues analysed. The data suggest that the hap gene product may be a novel ligand-responsive regulatory protein whose inappropriate expression in liver may relate to the hepatocellular carcinogenesis. 相似文献
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Mutual synergistic folding in recruitment of CBP/p300 by p160 nuclear receptor coactivators 总被引:11,自引:0,他引:11
Demarest SJ Martinez-Yamout M Chung J Chen H Xu W Dyson HJ Evans RM Wright PE 《Nature》2002,415(6871):549-553
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Identification of a second human retinoic acid receptor 总被引:119,自引:0,他引:119
N Brand M Petkovich A Krust P Chambon H de Thé A Marchio P Tiollais A Dejean 《Nature》1988,332(6167):850-853
We have previously described a human complementary DNA that encodes a novel protein which is homologous to members of the steroid/thyroid nuclear receptor multigene family. This novel protein (hap for hepatoma) exhibits strong homology with the human retinoic acid receptor (RAR) which has been recently characterized. To test the possibility that the hap protein might also be a retinoid receptor, a chimaeric receptor was created by replacing the putative DNA binding domain of hap with that of the human oestrogen receptor (ER). The resulting hap-ER chimaera was then tested for its ability to trans-activate an oestrogen-responsive reporter gene (vit-tk-CAT) in the presence of possible receptor ligands. Here we show that retinoic acid (RA) at physiological concentrations is effective in inducing the expression of this reporter gene by the hap-ER chimaeric receptor. This demonstrates the existence of two human retinoic acid receptors designated RAR-alpha and RAR-beta. 相似文献
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Receptors bound to antiprogestin from abortive complexes with hormone responsive elements 总被引:17,自引:0,他引:17
A Guiochon-Mantel H Loosfelt T Ragot A Bailly M Atger M Misrahi M Perricaudet E Milgrom 《Nature》1988,336(6200):695-698
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Retinoic acid regulates growth hormone gene expression 总被引:16,自引:0,他引:16
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