共查询到20条相似文献,搜索用时 27 毫秒
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Base sequence discrimination by zinc-finger DNA-binding domains 总被引:49,自引:0,他引:49
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Negative effect of the transcriptional activator GAL4 总被引:89,自引:0,他引:89
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Genetic evidence that zinc is an essential co-factor in the DNA binding domain of GAL4 protein 总被引:4,自引:0,他引:4
M Johnston 《Nature》1987,328(6128):353-355
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The POU domain is a bipartite DNA-binding structure 总被引:48,自引:0,他引:48
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Solution structure of the DNA-binding domain of the oestrogen receptor 总被引:37,自引:0,他引:37
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DNA recognition by GAL4: structure of a protein-DNA complex. 总被引:52,自引:0,他引:52
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Structure of the amino-terminal domain of Cbl complexed to its binding site on ZAP-70 kinase 总被引:13,自引:0,他引:13
Cbl is an adaptor protein that functions as a negative regulator of many signalling pathways that start from receptors at the cell surface. The evolutionarily conserved amino-terminal region of Cbl (Cbl-N) binds to phosphorylated tyrosine residues and has cell-transforming activity. Point mutations in Cbl that disrupt its recognition of phosphotyrosine also interfere with its negative regulatory function and, in the case of v-cbl, with its oncogenic potential. In T cells, Cbl-N binds to the tyrosine-phosphorylated inhibitory site of the protein tyrosine kinase ZAP-70. Here we describe the crystal structure of Cbl-N, both alone and in complex with a phosphopeptide that represents its binding site in ZAP-70. The structures show that Cbl-N is composed of three interacting domains: a four-helix bundle (4H), an EF-hand calcium-binding domain, and a divergent SH2 domain that was not recognizable from the amino-acid sequence of the protein. The calcium-bound EF hand wedges between the 4H and SH2 domains and roughly determines their relative orientation. In the ligand-occupied structure, the 4H domain packs against the SH2 domain and completes its phosphotyrosine-recognition pocket. Disruption of this binding to ZAP-70 as a result of structure-based mutations in the 4H, EF-hand and SH2 domains confirms that the three domains together form an integrated phosphoprotein-recognition module. 相似文献
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The function and structure of the metal coordination sites within the glucocorticoid receptor DNA binding domain 总被引:54,自引:0,他引:54
L P Freedman B F Luisi Z R Korszun R Basavappa P B Sigler K R Yamamoto 《Nature》1988,334(6182):543-546