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RP-HPLC法测定原料药中头孢拉定的含量
引用本文:蒋剑波.RP-HPLC法测定原料药中头孢拉定的含量[J].吉首大学学报(自然科学版),2003,24(3):94-96.
作者姓名:蒋剑波
作者单位:(吉首大学分析测试中心,湖南 吉首 416000)
摘    要:利用反相液相色谱法(RP-HPLC)直接测定原料药中头孢拉定的含量.采用YQG-ODS色谱柱,以V(CH3OH)∶V(4%HAc-NaAc)=3∶7的溶剂作流动相,于λ=262 nm处紫外检测,以峰面积外标法定量.头孢拉定在0.027~0.29 g·L-1浓度范围内具有良好的线性关系,回归方程为Y(μV·s)=1.88×107X(g·L-1)+20 876,r=0.998 3,平均回收率为99.7%,RSD为0.57%(n=3).

关 键 词:RP-HPLC  原料药  头孢拉定
文章编号:1007-2985(2003)03-0094-03
修稿时间:2003年5月15日

Determination of Cefradin by RP - HPLC
JIANG,Jian-Bo.Determination of Cefradin by RP - HPLC[J].Journal of Jishou University(Natural Science Edition),2003,24(3):94-96.
Authors:JIANG  Jian-Bo
Affiliation:(Center of Analysis & Measurement,Jishou University,Jishou 41600,Hunan China)
Abstract:A RP-HPLC method for the determination of cefradin in raw drug was established.A column of YQG-ODS was used with a mobile phase of V(CH3OH)∶V(4%HAc-NaAc)=3∶7 solution.The UV detector was set at 262 nm.The external standard method was used.There was a good linear correlation in the range of 0.027~0.29  g·L-1,Y(μV·s)=1.88×107X(g·L-1)+20 876,r=0.998 3.The average recovery was 99.7%,RSD was 0.57%(n=3).The elapsed time of a chromatographic analysis was 12 min.The method is rapid,accurate and reliable.
Keywords:RP-HPLC  cefradin  external standard method
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