| 林木RAPD标记技术研究进展 |
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| 引用本文： | 张日清,谭晓风,吕芳德.林木RAPD标记技术研究进展[J].吉首大学学报(自然科学版),2001,22(3):16-21. |
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| 作者姓名： | 张日清 谭晓风 吕芳德 |
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| 作者单位： | ( 中南林学院经济林育种与栽培重点实验室, 湖南 株洲 412006) |
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| 摘 要： | 综述了RAPD该标记在国内外林木遗传图谱构建、群体遗传多样性分析、系统进化研究、品种分类鉴定、DNA指纹分析和性状遗传品质改良等方面的研究进展和发展趋势, 指出随着理论和实验技术研究的不断深入, 分子标记技术将使林业生产迈上新的台阶.
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| 关 键 词： | RAPD标记 林木遗传育种 研究进展 |
| 文章编号： | 1007-2985(2001)03-0016-06 |
RAPD Marking and Its Application in Forest Genetics and Tree Breeding |
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| ZHANG Ri-Qing,TAN Xiao-Feng,LV Fang-De.RAPD Marking and Its Application in Forest Genetics and Tree Breeding[J].Journal of Jishou University(Natural Science Edition),2001,22(3):16-21. |
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| Authors: | ZHANG Ri-Qing TAN Xiao-Feng LV Fang-De |
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| Affiliation: | (Ministerial Key Lab of Economic Forest Tree Breeding and Cultivation, South Central Forestry University, Zhuzhou 412006, Hunan China) |
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| Abstract: | RAPD marking is derived from PCR methodology and procedures. It is similar to RFLP, SSR and AFLP to some extent but is obviously different from them in many aspects. One disadvantage of RAPD marking exists in the relatively low reproducibility, but its reliability as genetic markers has been verified in a large number of experiments. Optimizing the lab conditions and experimental protocols has proved to be instrumental in promoting the results reproducibility. RAPD marking has been extensively used in forest genetic studies and tree breeding trials, including construction of genetic linkage maps, analysis of population genetic structure and diversity, study of evolution systems, taxonomic identification, DNA fingerprinting and improvement of genetic traits. |
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| Keywords: | RAPD marking forest genetics and tree breeding literature review |
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