| 莲藕中多酚氧化酶的性质 |
| |
| 引用本文： | 黄建韶,田宏现.莲藕中多酚氧化酶的性质[J].吉首大学学报(自然科学版),2002,23(2):82-84. |
| |
| 作者姓名： | 黄建韶 田宏现 |
| |
| 作者单位： | (1.常德师范学院生物系，湖南 常德 415000;2.吉首大学生命科学与化学学院，湖南 吉首 416000) |
| |
| 摘 要： | 以冷冻丙酮制备莲藕多酚氧化酶丙酮粉，用分光光度法研究pH值、温度、底物浓度、抑制剂对酶活性的影响.结果表明，以邻苯二酚为底物，莲藕ppo的最适pH值为7.0，最适温度为35 ℃；Km为22.7 mmol/L，Vm为0.625 OD/min，100 ℃热处理25 s可钝化ppo的活性；抗坏血酸、亚硫酸钠为强烈抑制剂，1.75×10-4的抗坏血酸和3.00×10-4 的亚硫酸钠能有效抑制ppo的活性.
|
| 关 键 词： | 莲藕 多酚氧化酶 褐变 抑制剂 |
| 文章编号： | 1007-2985(2002)02-0082-03 |
| 修稿时间： | 2001年9月4日 |
Characteristics of Polyphenol Oxidase in Lotus Root |
| |
| HUANG Jian shao ,TIAN Hong xian.Characteristics of Polyphenol Oxidase in Lotus Root[J].Journal of Jishou University(Natural Science Edition),2002,23(2):82-84. |
| |
| Authors: | HUANG Jian shao TIAN Hong xian |
| |
| Affiliation: | (1.Dept.of Boilogy,Changde Normal College,Changde 415000,Hunan China;2.Dept.of Resources,Jishou University,Jishou 416000,Hunan China) |
| |
| Abstract: | The acetone powder of ppo in lotus root being extracted from ice?.cold acetone,the effects of pH,temperature,concentration of substrate,and inhibitors on ppo's activity were investigated by using the spectrophotometric method.The results showed that the optimal pH and tempearture were 7.0 and 35 ℃,Km and Vm of ppo with catechol substrate were 22.7 mmol/L and 0.625 OD/min respectively,the extinct condition of ppo was 25 s at 100 ℃,sodium sulfite and ascorbic acid were strong inhiitors,with the effective concentration being 3.00×10-4 and 1.75×10-4 respectively. |
| |
| Keywords: | lotus root polyphenol oxidase browning inhibitors |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《吉首大学学报(自然科学版)》浏览原始摘要信息 |
|
点击此处可从《吉首大学学报(自然科学版)》下载全文 |
