| 高粱种子醇溶蛋白的提取条件 |
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| 引用本文： | 王伟,印丽萍,陈沁.高粱种子醇溶蛋白的提取条件[J].上海大学学报(自然科学版),2007,13(6):746-750. |
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| 作者姓名： | 王伟 印丽萍 陈沁 |
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| 作者单位： | 1.上海大学 生命科学学院，上海 200444； 2.上海市出入境检验检疫局，上海 200135 |
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| 基金项目： | 上海科委基金资助项目（04DZ05003） |
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| 摘 要： | 以高粱种子为材料，研究了不同提取剂、提取剂用量、浓度和还原剂浓度对高粱种子醇溶蛋白提取的影响.蛋白浓度测定和SDS PAGE电泳表明，DDT浓度为4%的60％叔丁醇溶液，样品质量比提取剂用量为1∶8（g/mL）是提取高粱种子醇溶蛋白的最佳条件.该条件下，提取高粱属不同种的种子醇溶蛋白，得到的高粱属种子醇溶蛋白的“指纹”图谱，可以用来区分高粱属内的种间差异.
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| 关 键 词： | 醇溶蛋白 高粱 提取 |
| 文章编号： | 1007-2861（2007）06-0746-05 |
| 收稿时间： | 2006-09-22 |
| 修稿时间： | 2006年9月22日 |
Different Conditions of Sorghum Seed's Kafirin Extraction |
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| WANG Wei, YIN Li-ping, CHEN Qin.Different Conditions of Sorghum Seed's Kafirin Extraction[J].Journal of Shanghai University(Natural Science),2007,13(6):746-750. |
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| Authors: | WANG Wei YIN Li-ping CHEN Qin |
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| Affiliation: | 1. School of Life Sciences, Shanghai University, Shanghai 200444, China;;2. Shanghai Entry Exit Quarantine and Inspection Bureau, Shanghai 200135, China |
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| Abstract: | Effects of different reagents, dosages, concentrations, and reduction reagent concentrations on kafirin extraction from -Sorghum bicolor-were investigated. Measurements of protein concentration and the sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) showed that 60% τ butanol plus 4% DTT at a sample-weight to extraction-solution ratio of 1∶8 (g/mL) was optimum for kafirin extraction. Other sorghum species' alcohol solute protein (prolamin) were extracted under this condition. It is concluded that, by studying the protein “fingerprint”, different sorghum species can be discriminated from each other. |
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| Keywords: | sorghum kafirin extraction |
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