Solid lipid nanoparticles loading adefovir dipivoxil for antiviral therapy |
| |
| Authors: | Xing-guo Zhang Jing Miao Min-wei Li Sai-ping Jiang Fu-qiang Hu and Yong-zhong Du |
| |
| Affiliation: | [1]Department of Pharmacy, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; [2]College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China; [3]State Key Lab for Diagnosis and Treatment of lnfectious Diseases, Zhejiang University, Hangzhou 310003, China |
| |
| Abstract: | Herein, solid lipid nanoparticles (SLN) were proposed as a new drug delivery system for adefovir dipivoxil (ADV). The octadecylamine-fluorescein isothiocynate (ODA-FITC) was synthesized and used as a fluorescence maker to be incorporated into SLN to investigate the time-dependent cellular uptake of SLN by HepG2.2.15. The SLN of monostearin with ODA-FITC or ADV were prepared by solvent diffusion method in an aqueous system. About 15 wt% drug entrapment efficiency (EE) and 3 wt% drug loading (DL) could be reached in SLN loading ADV. Comparing with free ADV, the inhibitory effects of ADV loaded in SLN on hepatitis B surface antigen (HBsAg), hepatitis B e antigen (HBeAg) and hepatitis B virus (HBV) DNA levels in vitro were significantly enhanced. |
| |
| Keywords: | Adefovir dipivoxil （ADV） Solid lipid nanoparticles （SLN） Octadecylamine-fluorescein isothiocynate （ODA-FITC） Hepatitis B virus （HBV） |
| 本文献已被 维普 等数据库收录！ |
|
