| 三维培养基质体系在细胞迁移研究中的应用 |
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| 引用本文： | 余涛,文铁桥,黄海.三维培养基质体系在细胞迁移研究中的应用[J].上海大学学报(自然科学版),2009,15(5):546-550. |
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| 作者姓名： | 余涛 文铁桥 黄海 |
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| 作者单位： | 上海大学 生命科学学院,上海 200444 |
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| 基金项目： | 国家自然科学基金资助项目，上海市教委基金资助项目，上海大学系统生物研究基金 |
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| 摘 要： | 细胞迁移的研究中可用到的培养基质远不能满足需要.利用鼠尾胶和基底膜胶质(Matrigel)混合形成三维Collagen-Matrigel混合胶,该混合胶在培养液∶鼠尾胶∶基底膜胶质为1∶5∶4配比下,0.5 h内凝固满足组织培养要求.同时该混合胶经固定后在光学显微镜下结构维持良好,无颗粒.弥散性因子表达细胞团块和上菱唇组织块共培养于该三维Collagen-Matrigel混合胶培养基质中,结果显示细胞迁移良好,SDF-1因子和Slit因子分别对小脑上菱唇细胞具有化学吸引作用和化学排斥作用.
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| 关 键 词： | 三维培养基质 细胞迁移 化学趋化性 SDF-1因子 Slit因子 |
| 收稿时间： | 2008-05-29 |
Application of Three-Dimentional Mixture Gel in Cell Migration Research |
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| YU Tao,WEN Tie-Qiao,HUANG Hai.Application of Three-Dimentional Mixture Gel in Cell Migration Research[J].Journal of Shanghai University(Natural Science),2009,15(5):546-550. |
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| Authors: | YU Tao WEN Tie-Qiao HUANG Hai |
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| Affiliation: | School of Life Sciences, Shanghai University, Shanghai 200444, China |
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| Abstract: | The matrix used in cell culture cannot match the requirement during the cell migration research in vitro at present.The three-dimentional collagen-matrigel mixture gel was studied which was made up of culture medium: collagen: matrigel by different ratios.The mixture gel got solidified within half an hour at room temperature,and did not show visible particles under microscope observation after fixation.The results from co-culture of cell aggregate and upper rhombic lip (URL) explant indicated that SDF-1 has the chemoattraction to URL cells,while slit has the chemorepulsion to them. |
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| Keywords: | three-dimentional mixture gel cell migration chemotaxis SDF-1 slit |
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