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蛇源变形杆菌的分离鉴定
引用本文:李方青,曾维铭,周维官,陈业良,杨萍,韦平,毕朝斌,李振林.蛇源变形杆菌的分离鉴定[J].广西科学院学报,2000,16(3):135-137,141.
作者姓名:李方青  曾维铭  周维官  陈业良  杨萍  韦平  毕朝斌  李振林
作者单位:1. 广西卫生管理干部学院微生物实验室,南宁,530021
2. 广西科学院生物研究所,南宁,530003
3. 广西大学动物科技学院,南宁,530005
4. 广西畜牧局兽医处,南宁,530022
基金项目:广西自然科学基金,广西科学院科研项目,桂科自9912016,桂科院研9808,,
摘    要:取广西南宁、河池和柳州等地140条病死及濒死蛇的心血等作细菌培养。结果分离出细菌232株;分类鉴定发现79株为变形杆菌,占检出菌的34.1%;种的分类表明,63株为普通变形杆菌,16株为奇异变形杆菌;随机抽取其中5株进行毒素测定,均发现有肠毒素产生;做动物试验可致小白鼠死亡;做回归试验,可见蛇血带菌。说明,此变形杆菌具有一定的毒力,是造成蛇感染的常见菌之一,为人兽共患病的传染源,不可忽视。

关 键 词:  变形杆菌  分离  鉴定  肠毒素  细菌性疾病
收稿时间:2000/4/13 0:00:00
修稿时间:2000/6/19 0:00:00

Isolation and Identification of Proteus from Diseased Snakes
Li Fangqing,Zeng Weiming,Zhou Weiguan,Chen Yeliang,Yang Ping,Wei Ping,Bi Chaobin and Li Zhenlin.Isolation and Identification of Proteus from Diseased Snakes[J].Journal of Guangxi Academy of Sciences,2000,16(3):135-137,141.
Authors:Li Fangqing  Zeng Weiming  Zhou Weiguan  Chen Yeliang  Yang Ping  Wei Ping  Bi Chaobin and Li Zhenlin
Affiliation:Laboratory of Microbiology, Guangxi Health Administration College, Nanning, 530021,Laboratory of Microbiology, Guangxi Health Administration College, Nanning, 530021,Laboratory of Microbiology, Guangxi Health Administration College, Nanning, 530021,Laboratory of Microbiology, Guangxi Health Administration College, Nanning, 530021,Laboratory of Microbiology, Guangxi Health Administration College, Nanning, 530021,Laboratory of Microbiology, Guangxi Health Administration College, Nanning, 530021,Laboratory of Microbiology, Guangxi Health Administration College, Nanning, 530021 and Laboratory of Microbiology, Guangxi Health Administration College, Nanning, 530021
Abstract:232 bacteria were isolated from the heart blood samples of 140 die of illness or dying snakes collected in Nanning,Hechi and Liuzhou of Guangxi; The results of identification and classification revealed that 79 bacterial isolates belonged to the genus of Proteus, among them P. vulgaria was the dominant species with 63 isolates,and P. mirabilis with 16 isolates. Toxin test with 5 randomly chosen isolates showed positive. Pathogenicity tests with mouce and snake resulted in death and bacteremia respectively.The results demonstrated that proteus as a conditioned pathogen is an important agent in zoonosis.
Keywords:snake  proteus  isolation  identification  enterotoxin
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