| 镉对匍匐翦股颖成熟胚愈伤组织诱导及再生的影响 |
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| 引用本文： | 邓一飞,戴佳莺,吴雯君,张文举,陈沁,邓志瑞.镉对匍匐翦股颖成熟胚愈伤组织诱导及再生的影响[J].上海大学学报(自然科学版),2013,19(6):636-640. |
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| 作者姓名： | 邓一飞 戴佳莺 吴雯君 张文举 陈沁 邓志瑞 |
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| 作者单位： | 上海大学生命科学学院, 上海200444 |
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| 基金项目： | 上海大学创新基金资助项目(A10011210004) |
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| 摘 要： | 研究了不同浓度的镉离子对匍匐翦股颖愈伤组织形成、生长和植株再生的影响. 结果表明: 当培养基中镉离子浓度在2.50 mg/L 以下时, 愈伤组织的诱导频率高于对照, 并在浓度为2.50 mg/L 时高达83.3%, 说明低浓度镉离子对匍匐翦股颖愈伤组织的形成可能具有刺激效应; 随着镉离子浓度的进一步增加, 愈伤组织的诱导频率明显下降, 并在浓度大于50.00 mg/L 时完全抑制了愈伤组织的形成; 在愈伤组织生长初期(0~30 d), 镉离子浓度为12.50 mg/L 时, 愈伤组织生长形态最大, 随着愈伤组织继续生长(30~45 d), 低浓度(2.50 mg/L)的镉离子对愈伤组织生长的刺激效应强于更高浓度. 在植株再生过程中, 当镉离子浓度为1.25 mg/L 时的效果最佳, 再生苗生长最好.
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| 关 键 词： | 镉离子 匍匐翦股颖 愈伤组织 植株再生 |
Effects of Cadmium on Callus Induction and Regeneration from Mature Embryos of Creeping Bentgrasses |
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| DENG Yi-fei,DAI Jia-ying,WU Wen-jun,ZHANG Wen-ju,CHEN Qin,DENG Zhi-rui.Effects of Cadmium on Callus Induction and Regeneration from Mature Embryos of Creeping Bentgrasses[J].Journal of Shanghai University(Natural Science),2013,19(6):636-640. |
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| Authors: | DENG Yi-fei DAI Jia-ying WU Wen-jun ZHANG Wen-ju CHEN Qin DENG Zhi-rui |
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| Affiliation: | School of Life Sciences, Shanghai University, Shanghai 200444, China |
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| Abstract: | The effects of different cadmium ion concentrations on callus induction, growth and plantlet regeneration of creeping bentgrass were studied. When cadmium ion concentration in medium was below 2.50 mg/L, the induction frequency was higher than that of control. The induction frequency reached up to 83.3% when cadmium ion concentration in medium was 2.50 mg/L, indicating that low cadmium ion concentration may have stimulating effect on callus formation. As cadmium ion concentration increased, the induction frequency remarkably declined and callus formation was completely inhibited when cadmium ion concentration was over 50.00 mg/L. In the early days of the callus growth (0~30 d), the calli on the medium containing 12.50 mg/L cadmium ion were morphologically largest, and further prolonged growth (30~45 d) exhibited that one lower concentration (2.50 mg/L) gave stronger stimulating effect. During regeneration, regenerated seedlings grew best on the medium where 1.25 mg/L cadmium ion concerntration existed. |
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| Keywords: | cadmium ion callus creeping bentgrass plantlet regeneration |
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