| 室内新风空气净化系统微生物多样性研究 |
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| 引用本文： | 室内新风空气净化系统微生物多样性研究.室内新风空气净化系统微生物多样性研究[J].山东科学,2010,33(5):99-105. |
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| 作者姓名： | 室内新风空气净化系统微生物多样性研究 |
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| 作者单位： | 1. 齐鲁工业大学（山东省科学院）山东省科学院生物研究所，山东 济南 250103; 2. 山东省产品质量检验研究院，山东 济南 250102; |
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| 基金项目： | 国家重点研发计划（2017YFF0209900，2017YFF0209902） |
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| 摘 要： | 对济南市历城区使用不同时间的新风空气净化系统滤膜样本的细菌多样性进行检测，分析室内新风系统潜在的微生物二次污染风险。采集使用6至12个月的新风系统中超细玻璃纤维空气滤膜积累的微生物为样本，进行16S rDNA扩增子测序分析。结果显示：变形菌门、厚壁菌门、放线菌门和拟杆菌门为优势菌门，优势属有副球菌属、鞘氨醇单胞菌属、芽孢杆菌属、甲基杆菌属、双歧杆菌属等；随着滤膜使用时间的增加，优势菌门之间的相对丰度比例变化不明显，但总丰度呈现上升趋势，表明该地区室内空气微生物丰富且群落结构长期变化不大；使用12个月的样本中特别富含莱茵海默氏菌属、乳杆菌属和不动杆菌属，且条件致病菌奥斯陆莫拉氏菌的相对丰度较高，表明该地区的室内新风系统使用12个月时存在微生物二次污染风险。研究结果对居民防控室内空气二次污染起指导作用。
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| 关 键 词： | 室内空气 新风空气净化系统 微生物二次污染 细菌多样性 16S rDNA扩增子测序 |
| 收稿时间： | 2020-02-02 |
Study of microbial diversity in indoor fresh-air purification system |
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| YIN Xin,MENG Yi-wei,ZHAO Li-ya,QI Jun,XIA Xue-kui,GAO Cui-ling.Study of microbial diversity in indoor fresh-air purification system[J].Shandong Science,2010,33(5):99-105. |
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| Authors: | YIN Xin MENG Yi-wei ZHAO Li-ya QI Jun XIA Xue-kui GAO Cui-ling |
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| Affiliation: | 1.Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China; 2. Shandong Institute for Product Quality Inspection, Jinan 250102, China; |
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| Abstract: | We tested the bacterial diversity of filter membrane samples in fresh air purification systems in Licheng，Jinan，at different times, and analyzed the potential risk of secondary microbial contamination in indoor fresh-air purification systems.The bacterial diversity of three air-filtration membrane samples obtained from the fresh-air purification system used in Licheng at different times was analyzed using the 16S rDNA amplicon sequencing technology.The dominant bacterial phyla were Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. The dominant genera in three samples were Paracoccus, Sphingomonas, Bacillus, Methylobacterium,and Bifidobacterium. With the extension of the usage time of the fresh-air purification system, the relative abundance ratio among the dominant fungi did not change significantly, but the total abundance showed an increasing trend, indicating that the microorganism in indoor air was abundant in this area and the community structure did not change much in the long term. For the air-filtration membrane used in the area for 12 months, Rheinheimer, Lactobacillus, and Acinetobacter were also the dominant genera. Moreover, the relative abundance of opportunistic pathogen Moraxella osloensis was high, which indicatesthe indoor fresh-air purification system has a certain risk of microbial secondary pollution when it is used for 12 months. The results of this study can guide residents to prevent and control indoor-air secondary pollution. |
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| Keywords: | indoor air fresh-air purification system secondary microbial contamination bacterial diversity 16S rDNA amplicon sequencing technology |
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