PiggyBac(PB) transposon has become an effective tool for studying gene function, for it can be used for transgene and insertional mutagesis to explore gene function. Previous studies on the activity of PB transposon are mainly in vitro, and they primarily focused on transgenesis. In this study, PB binary cotransfection system was direct-ly delivered into liver via hydrodynamic injection using RFP (Red Fluorescence Protein) as the indicator of PB transposition. Tests were run 10 months later to detect whether the liver emitted red fluorescent. Then, genomic PCR and splinkerette PCR were performed to analyze the insertion sites of PB transposon. The results showed that PB transposon has an efficient transposition in liver as 34/68 insertion sites were located in gene sequence.