Accurate chromosome alignment and segregation depends on a series of precise molecular regulation, including the assembly and movement of microtubules. Deregulation of microtubules and chromosome movement can lead to genome instability and tumorigenesis. Many anti-microtubule chemotherapy drugs were applied in clinical research. In order to research more precisely on microtubules, we get stable expressed GFP-α-tubulin/GFP-CENP-A HeLa cell line though lentivirus infection system. We establish a technique that observing movement of microtubules in mitosis though time-lapse confocal scanning laser microscopy. That will increase our knowledge about tumorigenesis and anti-tumor drug research and development.