The percolation and transformation characteristics of ammonium bicarbonate (ABC) and long-effect ammonium bicarbonate (LEABC) in soil are important for carbon sequestration by the agricultural use of ABC or LEABC. The simulative soil columns were used to research the percolation and transformation of the two fertilizers in soil. It was found that the concentration of HCO_3- and CO_32- in soil columns was much higher and the irons percolated much deeper fertilized with LEABC than that of ABC. So the results indicate much more portions of the HCO_3- and CO_32- released from LEABC may percolate down into the aquifer and become sequestrated underground, and LEABC is better than ABC to sequestrate CO₂.