[ABSTRACT] Background: Curcumin is a natural compound isolated from Curcuma longa L. Recently, Curcumin is not only known for its antioxidant, free radical scavenger and anti-inflammatory activity, but the anti-carcinogenic properties. Objective: To identify the activity of Curcumin on the lung cancer cells’ sensitivity to anti-tumor agents. Methods: To declare the effect of Curcumin, the CCK-8 assays were performed in lung cancer cells, and the inhibition rates or IC50 values were calculated. Results: Pre-treatment of Curcumin up regulated the effect of anti-tumor agents: Gefitinib, Sunitinib, Gemcitabine and Paclitaxel. Conclusion By identified the roles of Curcumin in lung cancer cell; we demonstrated that Curcumin would participate in the reversion of multi-drug resistance process, and would be a novel therapy.