To obtain fine particulate matter concentration with high spatial resolution, three-dimensional atmospheric pollutant sampling platform based on unmanned aerial vehicle (UAV) technology was built. Research was conducted during a heavy haze pollution event in Lin’an city on November 2014. 3D distribution of PM2.5 concentration and synchronous meterology were successfully obtained. Research showed turbulence activity has great influence on the spatiotemporal distribution of PM2.5 concentration. Weak atmospheric vertical turbulence activity could cause stable atmospheric stratification during the morning and noon. The vertical dispersion of pollutants was obstructed by the inversion and isothermal layers in the atmosphere. The atmospheric turbulence enhanced at noon and the pollutant was well mixed, so that the vertical gradient of PM2.5 concentration was smaller than other times. At around 16:00, fine particulate matters transported horizontally and settled down, which means that the heavy pollution could mainly affected by the foreign sources of pollution.