Aiming at the problem of evaluating the effect of setting spacing of retro-reflective arch of new traffic safety facilities in Expressway tunnels, the driving simulation technology was used to evaluate the effectiveness of different spacing of reflective rings based on micro-driving behavior data. A tunnel driving simulation scenario based on virtual reality technology and 3D light environment technology was constructed by choosing a 3500m long tunnel as the research object. Thirty-three experienced drivers were selected to complete the driving simulation test. From the two aspects of behavior and vision, six evaluation indexes were selected, including speed, standard deviation of speed, acceleration, standard deviation of acceleration, accelerate power and pupil area, to construct a comprehensive evaluation system of tunnel retro-reflective arch. From the driver's point of view, the effects of different spacing retro-reflective arch in tunnels were quantified, and the matter element evaluation model based on extension theory was used to evaluate comprehensively. The results show that the influence of retro-reflective arch with different spacing in tunnels on drivers is significantly different. When the retro-reflective arch are 200 m, driving safety and stability are better, and their psychological relaxation is lower. The comprehensive evaluation results show that the safety and comfort of retro-reflective arch with 300 m and 400 m spacing are worse than those with 200 m spacing. Therefore, the optimum spacing of retro-reflective arch in tunnels is 200 m. The research results lay a theoretical foundation for the practical application of new traffic safety facilities in tunnels.