Objective: To study the effect of send oil from Zanthoxylum bungeanum Maxim and berberine hydrochloride on burn injury. Methods: The rat model with deep second-degree burns was set up and the therapeutic effects of the Zanthoxylum bungeanum Maxim send oil and berberine hydrochloride were evaluated through observing the wound healing time and ratio, and the pathological changes. Results: On the day 6, 12, 18 and 23 after burn, the healing ratio was markedly increased in the send oil group compared with the model group and the healing ratio in the combined treatment groups depended on the content of send oil. The time of decrustation in seed oil group were shorter than that in the model control (P <0.05). At 14 d after injury, send oil and berberine hydrochloride (8:1) group and seed oil group showed more fibroblast proliferation and more orderly arrangement of collagen fibers in dermis than the model group. At 21d after injury, in send oil group, newborn epidermises were connected into a film with thick epidermis compared with the model group. Conclusion: The send oil from Zanthoxylum bungeanum Maxim can speed up collagen synthesis, promote epithelial cell proliferation, and accelerate the healing of burn wound.